Group of molecular tags for optical nanoscopy
The Group is working on the creation of reporter systems adapted for live-cell nanoscopy. The group was formed in 2018.
- Protein-PAINT method: the reversible interaction of a genetically encoded reporter protein and a small-molecule fluorogenic dye freely penetrating the cytoplasmic membrane allows for high photostability and labeling density of target structures. We have developed green, orange, and red fluorogen-protein pairs suitable for localization microscopy (SMLM) as well as STED
- Protein-PAINT / IRES method: a fully genetically encoded version of protein-PAINT based on the reversible interaction of short peptides (K/E helices). The novel protein labeling system allows for high-density labeling of target structures in prolonged live-cell nanoscopy experiments.
- The use of spontaneous blinking of fluorescent proteins and dyes for nanoscopy
- Genetically encoded tags for STED and RESOLFT
- luminescence microscopy and imaging
|Alexander Mishin, Ph.D.||s. r. f.|
|Gavrikov A.S.||j. r. f.|
|Maxim Perfilov||j. r. f.|
|Shemiakina I.I.||j. r. f.|
|Gorshkova A.I.||t. q. - lab. as.|
Russia, Moscow, Ul. Miklukho-Maklaya 16/10 On the map