The Laboratory of Regulatory Transcriptomics conducts fundamental research in molecular biology and molecular microbiology. We have long and fruitfully studied the molecular mechanisms of adaptation of pathogenic bacteria (mainly the causative agent of tuberculosis, Mycobacterium tuberculosis) to persistence in the host organism, as well as molecular cascades that allow the infected macroorganism to provide immune protection against the pathogen. Various conditions, such as active tuberculosis and its chronic form, latent tuberculosis, dormant forms of mycobacteria, and their reactivation, are studied using in vitro and in vivo infection models.

The Laboratory studies the regulatory functions of noncoding RNAs in both pro- and eukaryotes. Much attention is paid to the non-coding transcriptome of mycobacteria as a regulator of adaptation to stresses. The regulatory role of small RNAs in the pathogenesis of mycobacteria is investigated.

Investigation of regulation of ribosomal proteins genes in bacteria, including mycobacteria, are being carried out.

The laboratory uses molecular biology methods to study the mechanismы of action of new antibacterial and antiviral compounds.

The research is conducted using methods of molecular biology, genetic engineering, microbiology, and molecular genetics. The professional competencies of the team include all methods of working with nucleic acids, next generation sequencing and the creation of mutant bacterial strains and eukaryotic cells with deleted target genes (knockout) and reduced expression levels (knockdown); proteomic studies of both individual proteins and omics approaches; methods of analysis and bioinformatics processing of the obtained results using statistical programs, including those adapted by the laboratory for specific tasks.

The laboratory cooperates with many laboratories and groups of IBCh, as well as Chemical and Biological Departments of Lomonosov Moscow State University, Federal Research Centre “Fundamentals of Biotechnology” RAS, Central Research Institute for Tuberculosis, Institute of Chemical Biology and Fundamental Medicine of the SB RAS.

Scientific projects

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Tatyana Azhikina

Russia, Moscow, Ul. Miklukho-Maklaya 16/10 — On the map