Russ. J. Bioorganic Chem., 2000, 26(3):207-212

A noninstrumental immunoassay based on colloidal dyes

Detecting labels based on water dispersions of colloidal textile dyes were developed that are useful in various analytical and diagnostic test systems for a simple visual assessment of the assay. Colored water-insoluble particles of dyes were used for the sorptional immobilization of streptavidin on their surface. The resulting streptavidin-dye (STR-DYE) complexes possessed a high visualizing capacity and were used for the combined detection of pesticides (simazine and 2,4-dichlorophenoxyacetic acid) by noninstrumental immunoassay (DYE-comb-assay, competitive dot-immunoassay in the comb format). The detection limits and the duration of our DYE-comb-assay (4 ng/ml, 20-25 min), HRP-comb-assay (competitive dot-immunoassay in the comb format using the enzymic conjugate of STR with horseradish peroxidase) (16 ng/ml), and the traditional competitive ELISA (12-16 ng/ml, 1.5 h) were compared. This DYE-comb-assay is simple enough and can be used under field conditions. © 2000 MAIK "Nauka/Interperiodica".

Lubavina IA, Salomatina IS, Zinchenko AA, Zherdev AV, Dzantiev BB

IBCH: 1697
Ссылка на статью в журнале: http://link.springer.com/10.1007/BF02786347
Кол-во цитирований на 06.2025: 3
Информация пока не проверена модераторами