Dr. Boldyrev had obtained his bachelor and master degree in Moscow Institute of Fine Chemical Technology. His PhD thesis was focused on synthetic lipids (fluorescent lipid probes). Excluding two post-doc visits (to Sweden and to China) it is already 20 years Dr. Boldyrev works in Shemyakin Institute of Bioorganic chemistry. His main research topic is lipids, their structure and role in membrane function and their indirect influence on protein/membrane interaction.
Dr. Boldyrev is studying lipids and lipid/protein interaction from the viewpoint of chemistry.
He had synthesized various lipid derivatives, e.g. a series of lipid probes based on a symmetrical bodipy fluorophore. The probes turned out to be very successful and were used in more than 2 dozen of published papers. At the moment, these probes are among the most studied membrane probes. Application examples: Biochim Biophys Acta. 1853 (4), 850-7; Langmuir 30 (11), 3154-64; Phys. Chem. Chem. Phys. 12, 6027-6034.
Another example of applying organic chemistry to better understanding biomembrane structure are model lipid compounds. Dr. Boldyrev had synthesized lipids with decreased conformational mobility and used them to investigate the role of conformational mobility in lipid aggregates behaviour. These leaded to the construction of lipid membranes ten times more resistant to breakdown than POPC membranes (Soft Matter, 2020,16, 3216-3223).
Boldyrev’s work with peripheral enzymes was started with construction of specific fluorescent probes (like a PLA2 probe (Biochem. Biophys. Res. Commun. 454 (1), 178-18.)) and evolved to more general research on protein/membrane interactions. The atomistic details of the PLA2 binding to bilayer interface were obtained (BBA-Biomembranes, 2021, 1863(1), 183481.) and non-enzymatic effect of hetero dimer phospholipase A2 on lipid membranes was discovered (Toxicon, 2017, 133, 169-179).