Laboratory of molecular technologies

In our research we apply synthetic biology principles to study redox signaling, to develop molecular tools for in vivo imaging, metabolic engineering and optogenetics. In addition, we study molecular mechanisms of ischemic pathology, signaling in cancer cells, an interplay between calcium and reactive oxygen species and other relevant topics. The central principle of our research is trans-species and even trans-kingdom transfer of molecular blocks in order to obtain engineered living system with new properties. Our achievements in the field of redox biology and fluorescent microscopy include development of sensors for important redox active compounds within living cells, novel tools for optogenetics, new methods of super resolution microscopy etc. 

- novel tools and instrumentation for thermogenetics (Fedotov et al, 2015; Safronov et al, 2015; Ermakova et al, 2017);

- a first application of a biosensor in subdiffraction microscopy (Mishina at al, 2015);

- SypHer2, a genetically encoded pH sensor allowing synaptic pH imaging (Matlashov et al, 2015);

- development of the red fluorescent genetically encoded H2O2 sensor (Ermakova et al, 2014);

- development of genetically encoded H2O2 generation/detection system based on fusion of D-amino acid oxidase and HyPer (Matlashov et al, 2014);

- development of the genetically encoded fluorescent sensor for intracellular NAD+/NADH ratio (Bilan et al, 2014);

- development of HyPer-3, the high dynamic range and fast-responding H2O2 probe for ratiometric and lifetime imaging (Bilan et al, 2013);

- first records of H2O2 production by phagocytosing macrophages at the level of single cell (Mishina et al, 2013);

- discovery of polarized H2O2 production in the immunological synapse (Mishina et al, 2012)

- discovery of H2O2 microdomains in the cells stimulated with growth factors, the first direct demonstration of local H2O2 signaling (Mishina et al., 2011);

- development of HyPer-2, improved version of HyPer with expanded dynamic range (Markvicheva et al., 2011);

- development of HyPer, sensor for H2O2 (Belousov et al, 2006).



Vsevolod Belousov

Russia, Moscow, Ul. Miklukho-Maklaya 16/10 — On the map