Aleksandr I. Gurevich

D.Sc (Chemistry), Professor

Selected publications

  1. Антонов К.В., Есипов Р.С., Гуревич А.И., Чувиковский Д.В., Микулинская Г.В., Феофанов С.А., Мирошников А.И. (2003). Химический и химико-ферментативный синтез α-тиотрифосфатов нуклеозидов. Bioorg. Khim. 29 (6), 616–622 ID:2091
  2. Esipov R.S., Gurevich A.I., Chuvikovsky D.V., Chupova L.A., Muravyova T.I., Miroshnikov A.I. (2002). Overexpression of Escherichia coli genes encoding nucleoside phosphorylases in the pET/Bl21(DE3) system yields active recombinant enzymes. Protein Expr. Purif. 24 (1), 56–60 [+]

    The Escherichia coli genes encoding purine nucleoside phosphorylase, uridine phosphorylase, and thymidine phosphorylase were cloned into pET plasmids to generate highly effective E. coli BL21(DE3) strains producing each of these enzymes. Optimum conditions for biosynthesis of each enzyme as a soluble protein with intact biological activity were found. The crude preparations are approximately 80% pure and can be used immediately for enzymatic transglycosylation. The enzyme preparations were purified to homogeneity by two steps including fractional precipitation with ammonium sulfate and subsequent chromatography on Sephadex G-100 and DEAE-Sephacel.

  3. Гуревич А.И., Аваков А.Э., Игошин А.В., Колосов М.Н. (1982). Выделение промоторов rrnb Escherichia coli. Биоорг. хим. 8 (4), 557–560 ID:244
  4. Gurevich A.I., Karapetyan M.G., Kolosov M.N., Korobko V.G., Onoprienko V.V., Popravko S.A., Shemyakin M.M. (1967). Synthesis of 12a-deoxy-5a,6-anhydrotetracycline. The first total synthesis of the naturally occurring tetracycline. Tetrahedron Lett. 2, 131–4 ID:249