Group of gene immuno-onco-therapy

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Irina Alekseenko

The expression of genes related to epithelial-mesenchymal transition (EMT) in colon cancer cells determines fibroblast activation and collagen remodeling

Collagen remodeling is closely related to the progression of tumors. Although it is carried out primarily by cancere-associated fibroblasts, the contribution of various cellular components to this process has not been adequately studied. We investigated the relationship between the EMT state of cancer cells, their ability to activate normal fibroblasts and remodel the extracellular matrix. Using immunofluorescence analysis, RNA-sequencing (RNA-seq) and second harmonic generation (SHG) microscopy, we were the first to describe the simultaneous changes in phenotypic, transcriptomic and remodeling activities in fibroblasts and cancer cells during their co-cultivation in vitro and in vivo, as well as the correlation of these changes with the phenotype of cancer cells. During co-cultivation, changes in the expression of many genes were observed, accompanied by the activation of fibroblasts and collagen matrix remodeling. Possible ligand-receptor interactions between fibroblasts and cancer cells have been determined. In collaboration with the Research Institute of Experimental Oncology and Biomedical Technologies PRMU

Publications

  1. Druzhkova I, Shirmanova M, Ignatova N, Dudenkova V, Lukina M, Zagaynova E, Safina D, Kostrov S, Didych D, Kuzmich A, Sharonov G, Rakitina O, Alekseenko I, Sverdlov E (2020). Expression of EMT-Related Genes in Hybrid E/M Colorectal Cancer Cells Determines Fibroblast Activation and Collagen Remodeling. Int J Mol Sci 21 (21),
  2. Alekseenko IV, Chernov IP, Kostrov SV, Sverdlov ED (2020). Are Synapse-Like Structures a Possible Way for Crosstalk of Cancer with Its Microenvironment? Cancers (Basel) 12 (4),