Group of molecular tags for optical nanoscopy

Department of Genomics and Postgenomic Technologies

Head: Alexander Mishin, Ph.D.
mishin@ibch.ru+7(495)995-55-57#2518

SMLM; smFRET; nanoscopy; fluorescence labaling

NamePositionContacts
Alexander Mishin, Ph.D.headmishin@ibch.ru+7(495)995-55-57#2518
Alexey GavrikovPhD stud.gavrikovalexey1@gmail.com

All publications (show selected)

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Alexander Mishin

New labels and approaches for low toxic fluorescent labeling of proteins in living cells

In collaboration with Group of chemistry of heterocyclic compounds

Ultraviolet, often used in fluorescence microscopy and nanoscopy, is extremely toxic to cells. Therefore, it is preferable to use labels in the green and red spectral regions.

We found the ability of the fluorescent protein mAvicFP1 to spontaneously blink under the influence of less toxic blue light, and applied this property to nanoscopy and to track single molecules of labeled proteins in living cells.

Fluorogen-activating proteins are new generation labeling systems based on transient interaction of a genetically encoded protein and  externally applied fluorogen. We have created and used  in living cells a new red fluorogen N871b for the FAST reporter protein.