Group of molecular tags for optical nanoscopy

Department of Biophotonics

Head: Alexander Mishin, Ph.D.
mishin@ibch.ru+7(495)995-55-57#2518

SMLM; smFRET; nanoscopy; fluorescence labaling

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The Group is working on the creation of reporter systems adapted for live-cell nanoscopy. The group was formed in 2018.

  • Protein-PAINT method: the reversible interaction of a genetically encoded reporter protein and a small-molecule fluorogenic dye freely penetrating the cytoplasmic membrane allows for high photostability and labeling density of target structures. We have developed green, orange, and red fluorogen-protein pairs suitable for localization microscopy (SMLM) as well as STED 

 

  • Protein-PAINT / IRES method: a fully genetically encoded version of protein-PAINT based on the reversible interaction of short peptides (K/E helices). The novel protein labeling system  allows for high-density labeling of target structures in prolonged live-cell nanoscopy experiments. 
  • The use of spontaneous blinking of fluorescent proteins and dyes for nanoscopy
  • Genetically encoded tags for STED and RESOLFT
  • luminescence microscopy and imaging 
NamePositionContacts
Alexander Mishin, Ph.D.headmishin@ibch.ru+7(495)995-55-57#2518
Alexey Gavrikovj. r. f.gavrikovalexey1@gmail.com
Maxim Perfilovj. r. f.sufrep@gmail.com
Irina Shemiakinaj. r. f.

All publications (show selected)

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Alexander Mishin

  • Russia, Moscow, Ul. Miklukho-Maklaya 16/10 — On the map
  • IBCh RAS, build. 34, office. 632
  • Phone: +7(495)995-55-57#2518
  • E-mail: mishin@ibch.ru

New labels and approaches for low toxic fluorescent labeling of proteins in living cells

In collaboration with Group of chemistry of heterocyclic compounds

Ultraviolet, often used in fluorescence microscopy and nanoscopy, is extremely toxic to cells. Therefore, it is preferable to use labels in the green and red spectral regions.

We found the ability of the fluorescent protein mAvicFP1 to spontaneously blink under the influence of less toxic blue light, and applied this property to nanoscopy and to track single molecules of labeled proteins in living cells.

Fluorogen-activating proteins are new generation labeling systems based on transient interaction of a genetically encoded protein and  externally applied fluorogen. We have created and used  in living cells a new red fluorogen N871b for the FAST reporter protein.