Group of molecular tags for optical nanoscopy
The Group is working on the creation of reporter systems adapted for live-cell nanoscopy. The group was formed in 2018.
- Protein-PAINT method: the reversible interaction of a genetically encoded reporter protein and a small-molecule fluorogenic dye freely penetrating the cytoplasmic membrane allows for high photostability and labeling density of target structures. We have developed green, orange, and red fluorogen-protein pairs suitable for localization microscopy (SMLM) as well as STED
- Protein-PAINT / IRES method: a fully genetically encoded version of protein-PAINT based on the reversible interaction of short peptides (K/E helices). The novel protein labeling system allows for high-density labeling of target structures in prolonged live-cell nanoscopy experiments.
- The use of spontaneous blinking of fluorescent proteins and dyes for nanoscopy
- Genetically encoded tags for STED and RESOLFT
- luminescence microscopy and imaging
|Alexander Mishin, Ph.D.||firstname.lastname@example.org, |
|Alexey Gavrikov||j. r. email@example.com|
|Maxim Perfilov||j. r. firstname.lastname@example.org|
|Irina Shemiakina||j. r. f.|
New labels and approaches for low toxic fluorescent labeling of proteins in living cells
In collaboration with Group of chemistry of heterocyclic compounds
Ultraviolet, often used in fluorescence microscopy and nanoscopy, is extremely toxic to cells. Therefore, it is preferable to use labels in the green and red spectral regions.
We found the ability of the fluorescent protein mAvicFP1 to spontaneously blink under the influence of less toxic blue light, and applied this property to nanoscopy and to track single molecules of labeled proteins in living cells.
Fluorogen-activating proteins are new generation labeling systems based on transient interaction of a genetically encoded protein and externally applied fluorogen. We have created and used in living cells a new red fluorogen N871b for the FAST reporter protein.
- (2019). Live-cell nanoscopy with spontaneous blinking of conventional green fluorescent proteins. Biochem Biophys Res Commun 522 (4), 852–854
- (2019). Red-shifted substrates for FAST fluorogen-activating protein based on the GFP-like chromophores. Chemistry 25 (41), 9592–9596