Group of molecular tags for optical nanoscopy
|Alexander Mishin, Ph.D.||firstname.lastname@example.org, |
|Alexey Gavrikov||PhD email@example.com|
New labels and approaches for low toxic fluorescent labeling of proteins in living cells
In collaboration with Group of chemistry of heterocyclic compounds
Ultraviolet, often used in fluorescence microscopy and nanoscopy, is extremely toxic to cells. Therefore, it is preferable to use labels in the green and red spectral regions.
We found the ability of the fluorescent protein mAvicFP1 to spontaneously blink under the influence of less toxic blue light, and applied this property to nanoscopy and to track single molecules of labeled proteins in living cells.
Fluorogen-activating proteins are new generation labeling systems based on transient interaction of a genetically encoded protein and externally applied fluorogen. We have created and used in living cells a new red fluorogen N871b for the FAST reporter protein.
- (2019). Live-cell nanoscopy with spontaneous blinking of conventional green fluorescent proteins. Biochem Biophys Res Commun ,
- (2019). Red-shifted substrates for FAST fluorogen-activating protein based on the GFP-like chromophores. Chemistry 25 (41), 9592–9596