Ерохина Софья Алексеевна

Аспирант (Лаборатория клеточных взаимодействий)

Эл. почта: sonya.erokhina@gmail.com


Период обученияСтрана, городУчебное заведениеДополнительная информация
1970 Россия, Москва Московский Государственный Университет им. М.В. Ломоносова

Гранты и проекты

ПериодДополнительная информация
2018–2018 Грант РФФИ №16-34-00836Получение, анализ свойств и роли в иммунном ответе субпопуляции HLA-DR-позитивных NK-клеток. Ерохина С., Стрельцова М., Каневский Л.
2018–2018 Грант РФФИ №16-04-01867Роль аллельных групп белка MICA, лиганда рецептора NKG2D, в регуляции противоопухолевой активности клеточного иммунитета. Ерохина С., Стрельцова М., Каневский Л., Коваленко Е., Абакушина Е., Клинкова А. 
2018–2018 Грант РНФ № 16-15-00309Оптимизация NK-клеток для адоптивного переноса при восстановлении пациентов после интенсивной противоопухолевой терапии.  Ерохина С., Стрельцова М., Каневский Л., Коваленко Е., Бойко А., Клинкова А., Сервули Е., Шустова О., Муравьева А. 
2018–2018 Грант РФФИ №17-04-00829Поиск механизма непосредственной активации NK-клеток человека бактериальными липополисахаридами. Ерохина С., Стрельцова М., Каневский Л., Коваленко Е.

Избранные публикации

  1. Streltsova M.A., Barsov E., Erokhina S.A., Kovalenko E.I. (2017). Retroviral gene transfer into primary human NK cells activated by IL-2 and K562 feeder cells expressing membrane-bound IL-21. J. Immunol. Methods 450, 90–94 [+]

    Natural killer (NK) cells are capable of rapidly recognizing and efficiently killing tumor cells. This makes them a potentially promising agent for cancer immunotherapy. Additional genetic modifications of NK cells may further improve their anti-tumor efficacy. Numerous technical challenges associated with gene delivery into NK cells have significantly tempered this approach. We achieved efficient retroviral vector transduction of primary human NK cells that were stimulated by a combination of IL-2 and engineered K562 cells expressing membrane-bound IL-21. The activated NK cells were in less differentiated state and expressed NK cell activation receptors NKG2D, NKp30, CD16, and were highly HLA-DR-positive. This NK cell population was highly susceptible to the transduction by both GFP- and NGFR-expressing retroviral vectors, with transduction efficiency exceeding 50%. More mature CD57(+) NK cell population was generally resistant to retroviral vector transduction because of poor response to the stimulation. Our findings may facilitate retroviral vector-mediated genetic engineering of human primary NK cells for future immunotherapies.

  2. Kovalenko E.I., Streltsova M.A., Kanevskiy L.M., Erokhina S.A., Telford W.G. (2017). Identification of Human Memory-Like NK Cells. Curr Protoc Cytom 79, 9.50.1–9.50.11 [+]

    Our understanding of NK biology is increased dramatically, a product of improved flow-cytometric techniques for analyzing these cells. NK cells undergo significant changes in repertoire during differentiation. A repeating stimulus, such as a cytomegalovirus infection, may result in accumulation of certain types of highly differentiated NK cells designated as memory-like, or adaptive NK cells. Adaptive NK cells are capable of rapid expansion and effective response to the recall stimulus. These cells differ significantly from conventional NK cells both functionally and phenotypically. Here we describe an approach for identification and analysis of adaptive NK cells in human peripheral blood. CD57-positive cells with high expression of activating-receptor NKG2C, increased expression of KIR receptors, lack of co-expression with inhibitory receptor NKG2A, and decreased expression of activating receptor NCR3 (NKp30) all characterize this cell type. The flow-cytometric method described below can identify this NK cell subset on a relatively simple flow cytometer. © 2017 by John Wiley & Sons, Inc.

  3. Kanevskiy L.M., Erokhina S.A., Streltsova M.A., Telford W.G., Sapozhnikov A.M., Kovalenko E.I. (2014). Bacterial lipopolysaccharide activates CD57-negative human NK cells. Biochemistry Mosc. 79 (12), 1339–48 [+]

    NK cells play an important regulatory role in sepsis by induction and augmentation of proinflammatory reactions in early stages of the septic process and by suppression of immune response in later stages of inflammation. The present work was aimed at the effect of bacterial lipopolysaccharide (LPS), the main pathogenic factor of sepsis development, on human NK cells ex vivo. We show that LPS activates immature CD57-negative NK cells, which typically constitute less than half of the normal NK cell population in human peripheral blood. Under conditions of NK cell stimulation with IL-2, addition of LPS provokes an increase in IFN-γ production. However, LPS both increased and inhibited NK cell cytotoxic activity. It is important to note that the activation of NK cells on LPS addition was observed in the absence of TLR4 on the NK cell surface. These results confirm our previous data arguing for a direct interaction of LPS with NK cells and evidence an atypical mechanism of LPS-induced NK cell activation without the involvement of surface TLR4.