Свирщевская Елена Викторовна

Кандидат биологических наук


Старший научный сотрудник (Лаборатория клеточных взаимодействий)

Тел.: +7 (495) 330-40-11

Эл. почта: esvir@mx.ibch.ru

Избранные публикации

  1. Zubareva A.A., Shcherbinina T.S., Varlamov V.P., Svirshchevskaya E.V. (2015). Intracellular sorting of differently charged chitosan derivatives and chitosan-based nanoparticles. Nanoscale 7 (17), 7942–52 [+]

    Chitosan (Chi) is a biodegradable nontoxic polycation with multiple reactive groups that is easily used to obtain derivatives with a desired charge and hydrophobic properties. The aim of this work was to study the intracellular traffic of positively charged hexanoyl-chitosan (HC) or HC-based nanoparticles (HCNPs) and negatively charged succinoyl-chitosan (SC) and SCNPs in epithelial and macrophage cell lines. By using flow cytometry we demonstrated that positively charged HC adhered to cell membranes quicker and more efficiently than negatively charged SC or NPs. However confocal studies showed that SC and SCNPs penetrated cells much more efficiently than HC while HCNPs did not enter the epithelial cells. Macrophages also phagocyted better negatively charged material but were able to engulf both HC and HCNPs. Upon entering the cells, SC and SCNPs were co-localized with endosomes and lysosomes while HC was found in mitochondria and, to a lesser extent, in lysosomes of epithelial cells. Macrophages, RAW264.7, more efficiently transported all Chi samples to the lysosomal compartment while some positively charged material was still found in mitochondria. Incubation of Chi derivatives and ChiNPs at pH specific to mitochondria (8.0) and lysosomes (4.5) demonstrated the neutralization of Chi charge. We concluded that epithelial cells and, to a lesser extent, macrophages sort charged material to the organelles neutralizing Chi charge.

    ID:1499
  2. Свирщевская Е.В., Матушевская Е.В., Чудаков Д.Б., Матушевская Ю.И. (2015). Роль инфекции в патогенезе аллергодерматозов. Клиническая дерматология и венерология  (2), 4–10 [+]

    Обзор обобщает данные о роли инфекции и нарушении целостности барьеров в развитии атопического дерматита.

    ID:1269
  3. Чудаков Д.Б., Свирщевская Е.В. (2015). Роль врождённого иммунитета при аллергии. Российский иммунологический журнал 9 (3), 298–306 [+]

    Аллергическая реакция I типа опосредована продукцией В-клетками иммуноглобулинов класса Е (IgE). Переключение В-клеток на синтез IgE вызывают интерлейкины (ИЛ) 4 и 13, которые продуцируются Т-хелперами 2 типа (Тх2). Однако ИЛ-4 и 13 могут продуцировать не только Тх2. Работы последних лет показали, что травма эпителия барьерных органов: желудочно-кишечного тракта, кожи и легких, приводит к продукции молекул, называемых тканевыми цитокинами, к которым относят ИЛ-25 и 33, а также тимический стромальный лимфопоэтин (ТСЛП). Тканевые цитокины, в свою очередь, активируют продукцию цитокинов, характерных для Тх2: ИЛ-4, 5 и 13, клетками, не несущими маркеров Тх2. Эти клетки получили название лимфоидных клеток врожденного иммунитета 2-го типа (ЛКВИ2) из-за экспрессии на их поверхности маркеров лимфоцитов CD44 и CD45 и способности синтезировать Тх2 цитокины. Повышение уровня ИЛ-4, 5 и 13 в крови в результате их синтеза ЛКВИ2 может вызывать переключение В-клеток на синтез IgE без участия классических Тх2, как это наблюдается при паразитарных инфекциях. В обзоре приведены основные характеристики тканевых и лимфоидных цитокинов, ассоциированных с аллергическим ответом, а также описание фенотипа и функций различных ЛКВИ2.

    ID:1409
  4. Kashirina E.I., Reshetov P.D., Alekseeva , Berzhets , Ryasantsev D.Y.u., Zubov , Chudakov D.B., Svirshchevskaya E.V. (2015). Encapsulation of allergens into Chitosan-Alginate Nanoparticles Prevents IgE binding. J. J. Vaccine Vaccination 1 (3), 012 [+]

    Allergens used for allergen-specific immunotherapy (ASIT) can induce side effects due to binding of IgE immobilized on mast cells to allergens. To prevent IgE binding, recombinant allergens from house dust mites (HDM) Dermatophagoides farinae Der f 2 or Aspergillus fumigatus (AF) fungi Asp f 3 were incapsulated into nanoparticles (NPs) developed from biodegradable polymers chitosan and alginate. Core NPs 400-450 nm in diameter and zeta potential +8 mV were prepared from amphiphilic chitosan derivative N-lauryl-N-succinoylchitosan (LSC). Allergens Der f 2 and Asp f 3 were introduced during core nanoparticles formation. To mask allergens additionally, allergen loaded core NPs were coated by alginate shell. As a result of polyelectrolyte interaction between positively charged LSC and negatively charged alginic acid core-shell type NPs were formed which were 450-550 nm in diameter and zeta potential -13 to -20 mV. We demonstrated that the encapsulation of allergens into core NPs significantly decreases IgE reactivity while core-shell NPs mask allergen completely, as was shown using IgE positive sera from patients allergic to HDMs or AF. Injection s.c. of both core and core-shell NPs into mice induced allergen specific IgGresponse comparable to free allergens. We concluded that encapsulation of allergens into core-shell NPs could increase the safety and preserve the efficacy of preparation for ASIT.

    ID:1411
  5. Чудаков Д.Б., Рязанцев Д.Ю., Каширина Е.И., Бержец В.М., Свирщевская Е.В. (2014). Роль дозы аллергена в индукции у мышей IgE антител на белки клещей домашней пыли. Иммунология 35 (6), 321–328 [+]

    Цель: изучение гуморального иммунного ответа на различные дозы белков клещей домашней пыли у мышей.

    Результаты: показана продукция антител класса IgE (но не IgG, IgA) при многократном введении низких доз (1 нг/инъекция) рекомбинантных белков. Продукция антител класса IgG и IgA возникала при введении относительно высоких (1-10 мкг) доз антигенов, при использовании адьювантов - начиная с 0,1 мкг. Антитела класса IgE были низкоаффинны.

    ID:1268
  6. Svirshchevskaya E.V., Prokhorov A.V., Zubareva A.A., Berkova N.P. (2014). Tumor immunology and immunotherapy. Recent Research Developments in Cancer 10, ID:1155
  7. Рязанцев Д.Ю., Дробязина П.Е., Хлгатян С.В., Завриев С.К., Свирщевская Е.В. (2014). Экспрессия аллергенов клещей домашней пыли Der f 1 и Der f 2 в листьях Nicotiana benthamiana. Биоорг. хим. 40 (4), . 468–478 ID:1157
  8. Kuznetsova N.R., Svirshchevskaya E.V., Skripnik I.V., Zarudnaya E.N., Benke A.N., Gaenko G.P., Molotkovskiĭ Yu.G., Vodovozova E.L. (2013). Interaction of liposomes bearing a lipophilic doxorubicin prodrug with tumor cells. Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology 7 (1), 12–20 [+]

    When used as nanosized carriers, liposomes enable targeted delivery and decrease systemic toxicity of antitumor agents significantly. However, slow unloading of liposomes inside cells diminishes the treatment efficiency. The problem could be overcome by the adoption of lipophilic prodrugs tailored for incorporation into lipid bilayer of liposomes. We prepared liposomes of egg yolk phosphatidylcholine and yeast phosphatidylinositol bearing a diglyceride conjugate of an antitumor antibiotic doxorubicin (a lipophilic prodrug, DOX-DG) in the membrane to study how these formulations interact with tumor cells. We also prepared liposomes of rigid bilayer-forming lipids, such as a mixture of dipalmitoylphosphatidylcholine and cholesterol, bearing DOX in the inner water volume, both pegylated (with polyethylene glycol (PEG) chains exposed to water phase) and non-pegylated. Efficiency of binding of free and liposomal doxorubicin with tumor cells was evaluated in vitro using spectrofluorimetry of cell extracts and flow cytometry. Intracellular traffic of the formulations was investigated by confocal microscopy; co-localization of DOX fluorescence with organelle trackers was estimated. All liposomal formulations of DOX were shown to distribute to organelles retarding its transport to nucleus. Intracellular distribution of liposomal DOX depended on liposome structure and pegylation. We conclude that the most probable mechanism of the lipophilic prodrug penetration into a cell is liposome-mediated endosomal pathway.

    ID:996
  9. Митрофанов В.С., Свирщевская Е.В. (2013). Аспергиллез легких. Изд. «Фолиант» , 183 ID:1167
  10. Svirshchevskaya E.V. (2012). Chronic immune response hypothesis for chronic fatigue syndrome: experimental results and literature overview. Chapter in a book "Immunosuppression" , 147–160 ID:1169
  11. Свирщевская Е.В., Гриневич Р.С., Решетов П.Д., Зубов В.П., Зубарева А.А., Ильина А.В., Варламов В.П. (2012). Наноносители на основе хитозана. Биотехносфера 19 (1), 13–23 ID:1175
  12. Зубарева А.А., Ильина А.В., Курек Д.В., Сизова С.В., Свирщевская Е.В., Варламов В.П. (2012). . Определение физико-химических параметров наночастиц модифицированного хитозана. Российские нанотехнологии 7 (1-2), 46–56 ID:1176
  13. Балабашин Д., ЗайцеваЗотова Д., Топорова В., Панина А., Марквичева Е., Свирщевская Е., Алиев Т. (2011). Способы увеличения продукции рекомбинантных антител в клеточных линиях CHO DG44. Современные проблемы науки и образования  (5), [+]

    The cell line CHO DG44 producing recombinant antibodies(Abs) to human tumor necrosis factor-alpha has been obtained. The influence of cell inoculation density and cultivation protocols on the level of Ab biosynthesis has been studied. The highest Ab yields have been observed at the inoculation density 3×106 cells/ml. The alternative method to cells-in-suspension cultivation has been proposed, which is the cell cultivation in calcium alginate hydrogel microgranules or alginate chitosan semipermeable microcapsules. It has been shown that the Ab production level by CHO DG44 cells entrapped into polymer microcapsules exceeds that of the cells-in-suspension cultivation regime.

    ID:540
  14. Moiseeva E.V., Kuznetsova N.R., Svirshchevskaya E.V., Bovin N.V., Sitnikov N.S., Shavyrin A.S., Beletskaya I.P., Combes S., Fedorov A.Y.u., Vodovozova E.L. (2011). Liposome formulations of combretastatin A4 and its 4-arylcoumarin analogue prodrugs: The antitumor effect in the mouse model of breast cancer. Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry 5 (3), 276–283 [+]

    The antimitotic agent combretastatin A-4 (CA-4) has been recently proposed as an antivascular agent for anticancer therapy. In order to reduce systemic toxicity by means of administration in liposome formulations, new lipophilic prodrugs, oleic derivatives of CA-4 and its 4-arylcoumarin analogue (CA4-Ole and ArC-Ole, respectively), have been synthesized in this study. Liposomes with mean diameter of 100 nm prepared on the basis of egg phosphatidylcholine and baker’s yeast phosphatidylinositol quantitatively included up to 15 mol% of CA4-Ole, or 7 mol% of ArC-Ole. To achieve targeting to neovascular endothelium prodrug bearing liposomes decorated with the tetrasaccharide selectin ligand Sialyl Lewis X (SiaLeX) have been also prepared. The antitumor activity was studied in vivo using the model of slow-growing mouse breast cancer. Under the dose used (22 mg/kg) and the administration protocol (four injections, one per a week, starting from the appearance of palpable tumors) cytostatic CA-4 did not reveal any anticancer effect; moreover, it even stimulated tumor growth. The liposome formulations of CA4-Ole did not demonstrate such stimulation. However, to achieve a pronounced antitumor effect, the number of injections of liposomes should be apparently increased. The cytotoxic activity of a novel antimitotic agent ArC was one order of magnitude lower in the human breast carcinoma cell culture in vitro. Nevertheless, in vivo in the mouse model of breast cancer the antitumor effect of this compound corresponded to the double equivalent dose of CA-4. The results demonstrate perspectives of SiaLeX-liposomes loaded with ArC-Ole: the preparation partially inhibited tumor growth already after the second injection. Thus, subsequent optimization of doses and regimens of administration both for ArC and liposomal ArC-Ole formulations are needed.

    ID:671
  15. Svirshchevskaya E.V., Shevchenko M.A., Huet D., Femenia F., Latgé J.P., Boireau P., Berkova N.P. (2009). Susceptibility of mice to invasive aspergillosis correlates with delayed cell influx into the lungs. Int. J. Immunogenet. 36 (5), 289–99 [+]

    Ubiquitous fungus Aspergillus fumigatus (A. fumigatus) is involved in invasive pulmonary aspergillosis (IPA), a frequent infection in immunocompromized patients. Genetic differences are likely to play a role predisposing to IPA. This study was aimed to compare six genetically different mouse strains in their susceptibility to IPA and to determine possible mechanisms involved in the pathogenesis of this infection. Immunosuppressed BALB/c and C57BL/6 mice infected with A. fumigatus conidia were more resistant to IPA than DBA/1, DBA/2, CBA, and A/Sn strains. Phagocytosis of A. fumigatus conidia by blood polymorphonuclear neutrophils (PMN) or bone marrow derived dendritic cells showed no difference between strains. All IPA susceptible strains demonstrated decreased PMN influx into the lungs during infection compared with resistant strains. Flow cytometry analysis of the composition of lung infiltrating cells showed that IPA susceptible mice had a decreased number of phagocytes before the infection. After infection the numbers of Gr-1(+)CD11b(+) PMN cells in the lungs of immunosuppressed mice increased from 10-20% to 50-60% while the percentage of CD11(+)F4/80(+) resident macrophages was unchanged. Among susceptible strains DBA/2 and A/Sn have a defect in C5 component of complement. Injection of normal serum into complement deficient but not into complement sufficient CBA or DBA/1 mice significantly improved their survival. We showed that complement replacement significantly increased PMN homing to the lungs of complement deficient mice. Thus, defect in complement system can predispose to IPA. Our results demonstrated that early influx of PMN into the lungs of mice is important for the resistance to IPA.

    ID:988
  16. Svirshchevskaya E.V., Alekseeva L.G., Reshetov P.D., Phomicheva N.N., Parphenyuk S.A., Ilyina A.V., Zueva V.S., Lopatin S.A., Levov A.N., Varlamov V.P. (2009). Mucoadjuvant properties of lipo- and glycoconjugated derivatives of oligochitosans. European journal of medicinal chemistry 44 (5), 2030–7 [+]

    Chitosan, (1-4)-2-amino-2-deoxy-beta-d-glucan, is a deacetylated form of chitin, an abundant biodegradable, positively charged natural polysaccharide. Chitosan is used for antigen delivery through mucosal barrier due to its ability to disrupt tight junctions. Here we produced new water-soluble low-molecular weight chitosan (LMW-Chi) lipid derivatives and compared their ability to stimulate humoral response with the effect of unmodified LMW-Chi or its oligosaccharide derivatives. LMW-Chi effectively penetrated into macrophage-like, lymphoid and epithelial cells. It also stimulated in mice IgG production to model proteins delivered either by subcutaneous or intranasal routes. Adjuvant effect of chitosan derivatives was comparable to or lower than that of unmodified LMW-Chi. Thus, it is possible that adjuvant effect is induced by unmodified glucosamine units of chitosan.

    ID:1185
  17. Svirshchevskaya E.V., Prokhorov A.V., Zubareva A.A., Berkova N.P. (2009). Tumor immunology and immunotherapy. Recent Research Developments in Cancer , ID:1154
  18. Svirshchevskaya E.V., Shevchenko M.A., Huet D., Femenia F., Latgé J.P., Boireau P., Berkova N.P. (2009). Susceptibility of mice to invasive aspergillosis correlates with delayed cell influx into the lungs. Int. J. Immunogenet. 36 (5), 289–299 ID:1184
  19. Svirshchevskaya E.V., Mariotti J., Wright M.H., Viskova N.Y., Telford W., Fowler D.H., Varticovski L. (2008). Rapamycin delays growth of Wnt-1 tumors in spite of suppression of host immunity. BMC Cancer 8, 176 [+]

    Rapamycin, an inhibitor of mammalian target of Rapamycin (mTOR), is an immunosuppressive agent that has anti-proliferative effects on some tumors. However, the role of Rapamycin-induced immune suppression on tumor progression has not been examined.

    ID:1190
  20. Svirshchevskaya E.V., Alekseeva L.G., Shevchenko M.A., Kurup V.P. (2008). Pathophysiology and immunotherapy in allergy: new perspectives. Chapter in book: Recent Trends on Aerobiology, Allergy and Immunology , 405–425 ID:1191
  21. Свирщевская Е.В., Алексеева Л.Г., Марченко А.М., Бержец В.М., Шевченко М.А., Андронова Т.М., Беневоленский С.В. (2006). Снижение продукции IgE рекомбинантными пептидами из основных белков аллергенов. Изд. «Фолиант»  (2), 91–97 ID:1205
  22. Мушенкова Н.В., Моисеева Е.В., Чаадаева А.М., Свирщевская Е.В. (2006). Индукция гуморального и клеточного ответа на муцин-1 с использованием ДНК иммунизации. Иммунология 27 (4), 216–221 ID:1210
  23. Svirshchevskaya E.V., Kurup V.P. (2005). Pathogenesis of allergy and specific features of allergic aspergillosis. In book: Mold Allergy, Biology and Pathogenesis , 257–274 ID:1206
  24. Свирщевская Е.В., Митрофанов В.С., Шендорова М.И., Чужова Н.М. (2005). Иммунитет при туберкулезе и аспергиллезе. Проблемы медицинской микологии 7 (1), 3–13 ID:1211
  25. Свирщевская Е.В., Шевченко М.А., Вискова Н.Ю., Шеховцова Е.Л. (2005). Повышение аффинности антител к основным белкам гриба Aspergullus fumigatus зависит от дозы антигена и формирования герминальных центров. Успехи медицинской микологии 5, 108–111 ID:1212
  26. Svirshchevskaya E.V., Viskova N., Shevchenko M., Alekseeva L., Marchenko A., Benevolensky S., Kurup V.P. (2004). High-affinity IgG to a major A. fumigatus allergen, Asp f 2, retards allergic response. Med. Sci. Monit. 10 (10), BR371–80 [+]

    Allergic diseases represent a major health threat to humans. Allergen-specific immunotherapy (SIT) is one of the significant approaches to the treatment of IgE-mediated allergy and its control. The mechanisms involved in SIT-induced responses are complex and still speculative. Immunological events associated with successful SIT include an increase in allergen-specific "blocking" IgG, reduction in cytokine production, and induction of regulatory or suppressor cells. The aim of this study was to estimate the effect of SIT using a single major allergen of A. fumigatus, Asp f 2, or its dominant B-cell epitope, aa254-268, in a murine model of allergic aspergillosis. It is known that A. fumigatus (Af), a ubiquitous fungus, is implicated in the pathogenesis of a number of clinically different allergic diseases.

    ID:992
  27. Шевченко М.А., Вискова Н.Ю., Свирщевская Е.В. (2004). Аллергенность и иммуногенность протеолитически деградированных белков из гриба Aspergillus fumigatus. Иммунология 1, 23–28 ID:1217
  28. Свирщевская Е.В., Попова И.С., Матушевская Е.В., Коцарева О.Д., Эртнеева И.Я. (2004). Плацебо-контролируемый эффект антигистаминного препарата кларотадин на продукцию ИЛ-13 при атопическом дерматите. Вестник дерматологии и венерологии 5, 27–32 ID:1218
  29. Svirshchevskaya E.V., Kurup V.P. (2003). Immunotherapy of allergic bronchopulmonary aspergillosis: a clinical and experimental approach. Front. Biosci. 8, s92–101 [+]

    Allergic bronchopulmonary aspergillosis (ABPA) is a severe allergic pulmonary complication caused by the saprophytic fungus Aspergillus fumigatus. The present review examines the pathogenesis of this disease describing in detail the role of innate and acquired immunity in the induction of sensitivity to A.fumigatus. Different approaches in developing specific immunotherapeutic treatments such as induction of anergy, regulatory cells, a switch from Th2 to Th1 type of immune response, CpG and genetic immunization and the usage of altered peptides or modified allergens are critically examined.

    ID:1220
  30. Marchenko A.N., Kozlov D.G., Svirshchevskaya E.V., Viskova N.Y., Benevolensky S.V. (2003). The p1 protein of the yeast transposon Ty1 can be used for the construction of bi-functional virus-like particles. J. Mol. Microbiol. Biotechnol. 5 (2), 97–104 [+]

    Virus-like particles (VLPs) containing heterologous proteins are often used as vaccines. Two approaches for the construction of bi-functional VLPs using hybrid protein pl-380 of the TY1 transposon of Saccharomyces yeast are described. We have shown that both C- and N-termini of p1-380 can be used for the expression of heterologous peptides. Peptides from A. Fumigatus Asp f 2, expressed at the C- and/or N-termini of p1-380, did not interfere with VLP self-assembling, were accessible for antibodies and hence were exposed at the VLP surface. Another way to obtain bivalent VLPs is the formation of mixed particles, which co-express two hybrid pl proteins with different heterologous protein fragments at the C-terminus. To do it the yeast cells were transfected with a mixture of two recombinant DNA coding Asp f 2 peptide and green fluorescent protein (Gfp). We have shown that both Asp f 2 peptide and Gfp are expressed within the same particle. To evaluate biological activity of bi-functional VLP a construction containing peptides representing dominant T- and B-cell epitopes of Asp f 2 was produced. Bi-functional particles were more potent in stimulating memory immune responses. These results demonstrate new possibilities of pl-380 based expression system to produce multifunctional VLPs.

    ID:1221
  31. Вискова Н.В., Шевченко М.А., Свирщевская Е.В. (2003). Индукция синтеза IgE при первичном ответе и ответе памяти на растворимые белки. Иммунология 24 (4), 227–231 ID:1224
  32. Свирщевская Е.В. (2003). Клинические и экспериментальные аспекты патогенеза аллергических реакций на условно патогенный грибок Aspergillus fumigatus. Иммунология  (3), 188–192 ID:1225
  33. Свирщевская Е.В., Матушевская Е.В., Коцарева О.Д., Багаева Л.В., Дзуцева И.Р., Лысенко А.А. (2003). Блокада акантолиза эпидермиса при вульгарной пузырчатке протективными антителами. Вестник дерматологии и венерологии  (4), 4–7 ID:1226
  34. Svirshchevskaya E.V., Alekseeva L., Marchenko A., Viskova N., Andronova T.M., Benevolenskii S.V., Kurup V.P. (2002). Immune response modulation by recombinant peptides expressed in virus-like particles. Clin. Exp. Immunol. 127 (2), 199–205 [+]

    Aspergillus fumigatus, a ubiquitous fungus, is implicated in the pathogenesis of a number of clinically different allergic diseases in man, including allergic bronchopulmonary aspergillosis. Peptide-based immunotherapy may offer an alternative treatment strategy for the management of allergic disease. The objective of this study was to alter the allergen-specific immune response using dominant T cell epitopes of a major A. fumigatus allergen, Asp f2, expressed in yeast as virus-like particles (VLP). The T cell epitopes of Asp f2, recognized in mice with an H-2d background, were determined by producing T-cell hybridomas. Two dominant T cell epitopes, aa60--71 and aa235--249, were identified and expressed in a yeast VLP system. To induce tolerance VLP-peptides were injected subcutaneously into mice previously immunized with recombinant Asp f2. The T cell immune response was abrogated totally in 3 weeks following a single injection of VLP but was restored 2 months later following intranasal antigen exposure. T-cell depletion resulted in the reduction of 20-30% of all antigen-specific immunoglobulin classes. Thus, recombinant peptides expressed in the VLP system can be used successfully in the modulation of Asp f2-induced immune response in mice, although a single administration is not sufficient to maintain a state of tolerance for a long period of time.

    ID:1228
  35. Svirshchevskaya E.V., Kurup V.P. (2002). IgE regulation and pathogenesis in ABPA. In book: Recent Res. Devel. Immunol. , 103–114 ID:1227
  36. Свирщевская Е.В., Вискова Н.Ю., Чудновская Т.Н., Гурина О.Н., Матушевская Е.В. (2002). Участие Т-хелперов 2 типа в патогенезе аутоиммунной пузырчатки. Иммунология  (2), 112–114 ID:1232
  37. Свирщевская Е.В., Вискова Н.Ю., Шевченко М.А. (2002). Механизмы патогенеза аллергического аспергиллеза. Аллергия, астма и клиническая иммунология  (4), 6–14 ID:1235
  38. Svirshchevskaya E.V., Alekseeva L.G., Andronova T.M., Kurup V.P. (2001). Do T helpers 1 and 2 recognize different class II MHC molecules? Humoral and cellular immune responses to soluble allergen from Aspergillus fumigatus Asp f2. Clin. Immunol. 100 (3), 349–54 [+]

    Cellular signals leading to T helper (Th)1/Th2 shift are not well known. Here we demonstrate that Th1 possibly recognizes peptides presented by the IE molecule of MHC class II while Th2 is activated by the recognition of peptides presented by the IA molecule. BALB/c mice immunized with Asp f2 developed stable IA-restricted Th2 immune response to the 12th day after immunization, as analyzed by IL-2 production. On the contrary, early Th0 cells did not secrete IL-2 upon Asp f2 stimulation but did produce a high level of IL-2 if stimulated in the presence of anti-IA Abs. This effect of anti-IA Abs on early Th0 cells was both MHC IE and CD4(+) cell restricted. In vivo blocking of Asp f2 peptide presentation by the IA molecule led to the formation of antigen-specific cytotoxicity as demonstrated using immune splenocytes as effector cells and Asp f2 loaded P815 cells as targets.

    ID:1236
  39. Рапопорт Е.М., Некрасов М.В., Хайдуков С.В., Свирщевская Е.В., Жигис Л.С., Козлов Л.В., Баталова Т.Н., Зубов В.П., Бовин Н.В. (2000). Изучение клеточной локализации галактозосвязывающего лектина из сыворотки крови человека. Биохимия 65 (11), 1558–1563 ID:207
  40. Viskova N.Y., Svirshchevskaya E.V., Sapoznikov A.M., Moiseeva E.V., Dizha V.I. (1998). Immunostimulatory activity of Milife, a novel immunomodulator of fungus origin. Immunopharmacol Immunotoxicol 20 (1), 119–33 [+]

    Milife is a novel immunomodulator derived from the fungus Fusarium Sambucium. In this study we examined immunomodulatory properties of Milife in 10 months-old BLRB mice. Milife was given to mice orally in a daily dose of 1 mg per mouse, for 2 to 6 days. Groups of mice were sacrificed on days 2, 4, and 6 of treatment, and 3 weeks after completion of a 6 days treatment with Milife, and lymphoid organs were obtained for analysis. Milife administration led to rapid and significant increase in total leukocyte and lymphocyte numbers in peripheral blood that persisted for at least 3 weeks after a 6 days treatment. Cellularity of lymph nodes, bone marrow and thymus increased significantly at days 4 and 6 of treatment, but returned to pretreatment levels after Milife discontinuation. Though total splenocyte numbers did not change dramatically, there occurred delayed increase in CD4+ cells in the spleen 3 weeks following treatment. Preferential accumulation of CD4+ cells was also consistently found in peripheral blood, with the peak being observed at day 6 of treatment. As a result, CD4/CD8 ratio in blood and spleen was significantly higher in treated than in untreated mice. Splenocytes from treated mice proliferated more vigorously in response to Con A. When added in vitro, Milife also mildly costimulated Con A-induced proliferation of splenocytes from intact animals. In conclusion, we have found that Milife can stimulate leuko- and lymphopoesis in BLRB mice, in particular, accumulation of CD4+ T cells in peripheral lymphoid organs. We conclude that Milife may represent an immunomodulator with the potential to correct T cell dysfunction in patients with immunodeficiency.

    ID:890