Рязанцев Дмитрий Юрьевич

Премии и заслуги

- Лауреат стипендии Президента РФ, 2015 г.

- Диплом за лучший стендовый доклад на II Международной научной конференции «Генетика и биотехнология XXI века: проблемы, достижения, перспективы», посвященной 50-летию Института генетики и цитологии НАН Беларуси, которая состоится 13-16 октября 2015 года в г. Минск, Беларусь.

- Член экспертной коллегии фонда Сколково в области агробиотехнологий.

Гранты и проекты

ПериодДополнительная информация
«Разработка технологии получения компонентов и создание тест-систем для определения токсинов и патогенов на основе методов иммуно-ПЦР и мультиплексного флуоресцентного иммуноанализа по технологии хМАР», шифр «Биотест-ИБХ РАН», в рамках Государственного контракта № 10411.0810200.13.В25 от 22.07.2010 г. на тему: «Разработка технологии производства тест-систем на основе иммуно-ПЦР, биочипов и мультиплексного флуоресцентного иммуноанализа с созданием лабораторной базы в соответствии с требованиями GMP» (шифр «Биотест»). ГК № 10411.0810200.13.В25/1 от 23. 07.10 НИОКР, заказчик Минпромторг. 2010г.
«Создание искусственных конструкций для лечения аллергий и исследования аллергенности веществ» по теме: «Разработка синтетических пептидных мультивалентных вакцин для лечения аллергий на основе В-эпитопов аллергенов и универсальных Т-эпитопов вируса гриппа H1N1». В рамках федеральной целевой программы «Исследования и разработки по приоритетным направлениям развития научно-технологического комплекса России на 2007-2012 годы» научно-исследовательские работы по лоту шифр «2011-1.2-512-017».
«Разработка биочипа и тест-систем для определения белковых токсинов, включая стафилококковые энтеротоксины, и антидота против ботулинического нейротоксина А, а так же ПЦР-тест систем для выявления ряда значимых патогенов растений», в рамках договора № 34-405/07 от 05.10.2007 по Госконтракту № ГП/07/538/НТБ/К от 11.09.2007 г. Шифр «Биопатоген». ГК № 34-405/07 от 05.10.2007, заказчик Минпромторг. 2007-2009гг.
Проект международного научно-технического центра ISTC #3978. «Организация Лаборатории молекулярной диагностики во Всероссийском научно-исследовательском институте фитопатологии для диагностики и идентификации патогенов растений из Российской Государственной Коллекции фитопатогенных микроорганизмов». 2009-2012гг.

Избранные публикации

  1. Morozov S.Y., Miliytina I.A., Bobrova V.K., Ryazantsev D.Y., Erokhina T.N., Zavriev S.K., Agranovsky A.A., Solovyev A.G., Troitsky A.V. (2015). Structural evolution of the 4/1 genes and proteins in non-vascular and lower vascular plants. Biochimie , [+]

    The 4/1 protein of unknown function is encoded by a single-copy gene in most higher plants. The 4/1 protein of Nicotiana tabacum (Nt-4/1 protein) has been shown to be alpha-helical and predominantly expressed in conductive tissues. Here, we report the analysis of 4/1 genes and the encoded proteins of lower land plants. Sequences of a number of 4/1 genes from liverworts, lycophytes, ferns and gymnosperms were determined and analyzed together with sequences available in databases. Most of the vascular plants were found to encode Magnoliophyta-like 4/1 proteins exhibiting previously described gene structure and protein properties. Identification of the 4/1-like proteins in hornworts, liverworts and charophyte algae (sister lineage to all land plants) but not in mosses suggests that 4/1 proteins are likely important for plant development but not required for a primary metabolic function of plant cell.

    ID:1343
  2. Beyshova I.S., Chuzhebaeva G.D., Aubakirov M.Z.h., Kozhmuhametova A.S., Stakheev A.A., Ryazantsev D.Y., Zavriev S.K., Oleynik A.T. (2015). Development of PCR diagnosis of pathogenic fungi of the genus Septoria affecting cereal crops in Northern Kazakhstan. . Biosciences Biotechnology Asia 12 (2), 1321 [+]

    Traditional methods of complex diagnostics, including the selection of species in pure culture, selection environments and cultivation conditions, obtaining monoporosa isolates and microscopy are long, time-consuming and ineffective. Immune-enzyme analysis method takes a few hours, however, developed on the basis of the methodology of species-specific, predictive and quantification S. Tritici and S. nodorum in the leaves and seeds of wheat insensitive do not always provide a clear identification of these species. The introduction of express methods of diagnostics of Septoria leaf blotch on the basis of the polymerase chain reaction (PCR) in modifications of Real-Time lets quickly and accurately diagnose the disease. A system of highly specific sensitive PCR diagnostics of pathogenic fungi Septoria tritici and Stagonospora nodorum, causing diseases of cereals in Northern Kazakhstan is developed. The primers and probes are designed based on partial sequences of the internal transcribed spacer of ribosomal DNA (ITS) provides a quick and accurate identification of pathogens Septoria by quantitative PCR without the risk of contamination of the work area with amplification products. Effectiveness of the test-system was also demonstrated in samples of total DNA isolated from the infected herbarium material.

    ID:1426
  3. Чудаков Д.Б., Рязанцев Д.Ю., Каширина Е.И., Бержец В.М., Свирщевская Е.В. (2014). Роль дозы аллергена в индукции у мышей IgE антител на белки клещей домашней пыли. Иммунология 35 (6), 321–328 [+]

    Цель: изучение гуморального иммунного ответа на различные дозы белков клещей домашней пыли у мышей.

    Результаты: показана продукция антител класса IgE (но не IgG, IgA) при многократном введении низких доз (1 нг/инъекция) рекомбинантных белков. Продукция антител класса IgG и IgA возникала при введении относительно высоких (1-10 мкг) доз антигенов, при использовании адьювантов - начиная с 0,1 мкг. Антитела класса IgE были низкоаффинны.

    ID:1268
  4. Ryazantsev D.Y., Kvach M.V., Tsybulsky D.A., Prokhorenko I.A., Stepanova I.A., Martynenko Y.V., Gontarev S.V., Shmanai V.V., Zavriev S.K., Korshun V.A. (2014). Design of molecular beacons: 3' couple quenchers improve fluorogenic properties of a probe in real-time PCR assay. Analyst 139 (11), 2867–72 [+]

    Convenient preparation of fluorogenic hairpin DNA probes (molecular beacons) carrying a pair of FAM fluorophores (located close to 5'-terminus of the probe) or a pair of BHQ1 quenchers on 3'-terminus (with (BHQ1)2 or BHQ1-BHQ1 composition) is reported. These probes were used for the first time in a real-time PCR assay and showed considerable improvements in fluorogenic properties (the total fluorescence increase or signal-to-background ratio) in assay conditions vs. conventional one-FAM-one-BHQ1 molecular beacon probes as well as vs. hydrolyzable one-FAM-one-BHQ1 TaqMan probes. At the same time, such multiple modifications of the probe do not influence its Cq (a fractional PCR cycle used for quantification). The probe MB14 containing a BHQ1-BHQ1 pair showed a PCR fluorescence/background value of 9.6 which is more than two times higher than that of a regular probe MB2 (4.6). This study demonstrates prospects for the design of highly fluorogenic molecular beacon probes suitable for quantitative real-time PCR and for other potential applications (e.g. intracellular RNA detection and SNP/mutation analysis).

    ID:1019
  5. Doronin I.I., Vishnyakova P.A., Kholodenko I.V., Ponomarev E.D., Ryazantsev D.Y., Molotkovskaya I.M., Kholodenko R.V. (2014). Ganglioside GD2 in reception and transduction of cell death signal in tumor cells. BMC Cancer 14, 295 [+]

    Ganglioside GD2 is expressed on plasma membranes of various types of malignant cells. One of the most promising approaches for cancer immunotherapy is the treatment with monoclonal antibodies recognizing tumor-associated markers such as ganglioside GD2. It is considered that major mechanisms of anticancer activity of anti-GD2 antibodies are complement-dependent cytotoxicity and/or antibody-mediated cellular cytotoxicity. At the same time, several studies suggested that anti-GD2 antibodies are capable of direct induction of cell death of number of tumor cell lines, but it has not been investigated in details. In this study we investigated the functional role of ganglioside GD2 in the induction of cell death of multiple tumor cell lines by using GD2-specific monoclonal antibodies.

    ID:1112
  6. Рязанцев Д.Ю., Дробязина П.Е., Хлгатян С.В., Завриев С.К., Свирщевская Е.В. (2014). Экспрессия аллергенов клещей домашней пыли Der f 1 и Der f 2 в листьях Nicotiana benthamiana. Биоорг. хим. 40 (4), . 468–478 ID:1157
  7. Rogozhin E.A., Ryazantsev D.Y., Grishin E.V., Egorov T.A., Zavriev S.K. (2012). Defense peptides from barnyard grass (Echinochloa crusgalli L.) seeds. Peptides 38 (1), 33–40 [+]

    A number of defense polypeptides from latent seeds of weed cereal barnyard grass (Echinochloa crusgalli L.) has been isolated and characterized using an acidic extraction and high performance liquid chromatography methods in combination with MALDI-TOF mass spectrometry and Edman sequencing. Members of three antimicrobial peptide families and two protease inhibitor families were found to be localized in barnyard grass seeds. Their biological activity concerning to Gram-Positive and Gram-Negative phytopathogenic bacteria, as well as oomycete Phytophthora infestans, has been investigated. Diversity of barnyard grass defense peptides is a significant factor that provides a resistance of E. crusgalli seeds to germination and latent phases.

    ID:805
  8. Ryazantsev D.Y., Tsybulsky D.A., Prokhorenko I.A., Kvach M.V., Martynenko Y.V., Philipchenko P.M., Shmanai V.V., Korshun V.A., Zavriev S.K. (2012). Two-dye and one- or two-quencher DNA probes for real-time PCR assay: synthesis and comparison with a TaqMan™ probe. Analytical and bioanalytical chemistry 404 (1), 59–68 [+]

    A typical TaqMan™ real-time PCR probe contains a 5'-fluorescent dye and a 3'-quencher. In the course of the amplification, the probe is degraded starting from the 5'-end, thus releasing fluorescent dye. Some fluorophores (including fluorescein) are known to be prone to self-quenching when located near each other. This work is aimed at studying dye-dye and dye-quencher interactions in multiply modified DNA probes. Twenty-one fluorogenic probes containing one and two fluoresceins (FAM), or a FAM-JOE pair, and one or two BHQ1 quenchers were synthesized using non-nucleoside reagents and "click chemistry" post-modification on solid phase and in solution. The probes were tested in real-time PCR using an ~300-bp-long natural DNA fragment as a template. The structural prerequisites for lowering the probe background fluorescence and increasing the end-plateau fluorescence intensity were evaluated and discussed.

    ID:753
  9. Ryazantsev D.Y.u., Petrova E., Kalinina N.A., Valyakina T.I., Grishin E.V., Zavriev S.K. (2012). Application of supramolecular DNA-streptavidin complexes for ultrasensitive detection of several toxins by immuno-PCR. 14. Global J. Anal. Chem. 3 (17), [+]
    ID:650
  10. Стахеев А.А., Рязанцев Д.Ю., Завриев С.К. (2011). Выявление новых генетических маркеров для таксономической характеристики и идентификации грибов рода Fusarium. Биоорг. хим. 37, 662–671 [+]
    ID:649
  11. Kapustin D.V., Prostyakova A.I., Ryazantsev D.Y., Zubov V.P. (2011). Novel composite matrices modified with nanolayers of polymers as perspective materials for separation of biomolecules and bioanalysis. Nanomedicine (Lond) 6 (2), 241–55 [+]

    A new approach for the preparation of adsorbents for one-step isolation/purification of DNA from different samples (e.g., bacterial lysates, smears and blood) has been developed.

    ID:458
  12. Stakheev A.A., Ryazantsev D.Y.u., Gagkaeva T.Y.u., Zavriev S.K. (2010). PCR detection of Fusarium fungi with similar profiles of the produced mycotoxins. Food Control 22 (3-4), 462–468 [+]
    ID:648
  13. Рязанцев Д.Ю., Абрамов Д.Д., Завриев С.К. (2009). Диагностика карантинных фитопатогенов методом ПЦР в формате FLASH. Сельскохозяйственная биология  (3), 114–117 ID:161
  14. Рязанцев Д.Ю., Завриев С.К. (2009). Эффективный метод диагностики и идентификации вирусных патогенов картофеля. Мол. биол. 43 (3), 558–567 ID:160
  15. Абрамова С.Л., Рязанцев Д.Ю., Воинова Т.М., Завриев С.К. (2008). Диагностика фитопатогенных грибов Septoria tritice и Stragonaspora nodorum методом FLASH–ПЦР. Биоорг. хим. 34, 107–113 ID:158
  16. Рязанцев Д.Ю., Абрамова С.Л., Евстратова С.В., Гагкаева Т.Ю., Завриев С.К. (2008). Диагностика токсиногенных грибов рода Fusarium методом FLASH-PCR. Биоорг. хим. 35, 799–807 ID:29