Михалева Инесса Ивановна

Кандидат химических наук


Ведущий научный сотрудник (Лаборатория химии пептидов)

Тел.: +7 (495) 3355366

Эл. почта: inessamikh@rambler.ru

Образование

Период обученияСтрана, городУчебное заведениеДополнительная информация
1958–1963 Москва МГУ химфак МГУ, кандидат хим. наук доцент медаль "За трудовую доблесть"

Избранные публикации

  1. Mikhaleva I.I., Prudchenko I.A., Ivanov V.T., Voitenkov V.B. (2009). JmjC-domain-containing histone demethJmjC-domain-containing histoneylases of the JMJD1B type as putative precursors of endogenous DSIP.ylases of the JMJD1B type as putative precursors of endogenous DSIP. Peptides 24 (1), 826–831 ID:803
  2. Румш Л.Д., Михайлова А.Г., Михура И.В., Прудченко И.А., Чикин Л.Д., Михалева И.И., Калиберда Е.Н., Дергоусова Н.И., Мельников Э.Э., Формановский А.А. (2008). Селективные ингибиторы плазмепсина II Plasmodium falciparum на основе пепстатина. Биоорг. хим. 34 (6), 739–746 ID:120
  3. Михалева И.И., Рихирева Г.Т., Прудченко И.А., Голубев И.Н. (2006). Взаимодействие дельта-сон индуцирующего пептида и его аналогов с клеточными мембранами и структурно- функциональный анализ. Биоорг. хим. 32 (2), 176–182 ID:229
  4. Afonin P.V., Fokin A.V., Tsygannik I.N., Mikhailova I.Y., Onoprienko L.V., Mikhaleva I.I., Ivanov V.T., Mareeva T.Y., Nesmeyanov V.A., Li N., Pangborn W.A., Duax W.L., Pletnev V.Z. (2001). Crystal structure of an anti-interleukin-2 monoclonal antibody Fab complexed with an antigenic nonapeptide. Protein Sci. 10 (8), 1514–21 [+]

    The three-dimensional structure of the Fab fragment of a monoclonal antibody (LNKB-2) to human interleukin-2 (IL-2) complexed with a synthetic antigenic nonapeptide, Ac-Lys-Pro-Leu-Glu-Glu-Val-Leu-Asn-Leu-OMe, has been determined at 3.0 A resolution. In the structure, four out of the six hypervariable loops of the Fab (complementarity determining regions [CDRs] L1, H1, H2, and H3) are involved in peptide association through hydrogen bonding, salt bridge formation, and hydrophobic interactions. The Tyr residues in the Fab antigen binding site play a major role in antigen-antibody recognition. The structures of the complexed and uncomplexed Fab were compared. In the antigen binding site the CDR-L1 loop of the antibody shows the largest structural changes upon peptide binding. The peptide adopts a mostly alpha-helical conformation similar to that in the epitope fragment 64-72 of the IL-2 antigen. The side chains of residues Leu 66, Val 69, and Leu 70, which are shielded internally in the IL-2 structure, are involved in interactions with the Fab in the complex studied. This indicates that antibody-antigen complexation involves a significant rearrangement of the epitope-containing region of the IL-2 with retention of the alpha-helical character of the epitope fragment.

    ID:89