Генералова Алла Николаевна

Личная информация

Генералова Алла Николаевна — научный сотрудник, кандидат химических наук. Активно участвует в подготовке студентов и аспирантов.


Период обученияСтрана, городУчебное заведениеДополнительная информация
1983–1988 Россия, Москва Московский институт тонкой химической технологии им. М.В. Ломоносова Диплом химика
2000 Россия, Москва Институт биоорганической химии им. М.М. Шемякина и Ю.А. Овчинникова Диплом кандидата химических наук

Научные интересы

Она является специалистом в области синтеза полимерных частиц для иммуноанализа, содержащих цветные, флуоресцентные или магнитные метки; модификации поверхности полимерных частиц; новых методов на основе реакции латексной агглютинации; биоспецифических реакций, межфазных слоев, монослоев.

Избранные публикации

  1. Generalova A.N., Kochneva I.K., Khaydukov E.V., Semchishen V.A., Guller A.E., Nechaev A.V., Shekhter A.B., Zubov V.P., Zvyagin A.V., Deyev S.M. (2015). Submicron polyacrolein particles in situ embedded with upconversion nanoparticles for bioassay. Nanoscale 7 (5), 1709–17 [+]

    We report a new surface modification approach of upconversion nanoparticles (UCNPs) structured as inorganic hosts NaYF4 codoped with Yb(3+) and Er(3+) based on their encapsulation in a two-stage process of precipitation polymerization of acrolein under alkaline conditions in the presence of UCNPs. The use of tetramethylammonium hydroxide both as an initiator of acrolein polymerization and as an agent for UCNP hydrophilization made it possible to increase the polyacrolein yield up to 90%. This approach enabled the facile, lossless embedment of UCNPs into the polymer particles suitable for bioassay. These particles are readily dispersible in aqueous and physiological buffers, exhibiting excellent photoluminescence properties, chemical stability, and also allow the control of particle diameters. The feasibility of the as-produced photoluminescent polymer particles mean-sized 260 nm for in vivo optical whole-animal imaging was also demonstrated using a home-built epi-luminescence imaging system.

  2. Guller A.E., Generalova A.N., Petersen E.V., Nechaev A.V., Trusova I.A., Landyshev N.N., Nadort A., Grebenik E.A., Deyev S.M., Shekhter A.B., Zvyagin A.V. (2015). Cytotoxicity and non-specific cellular uptake of bare and surface-modified upconversion nanoparticles in human skin cells. Nano Research 8 (5), 1546–1562 [+]

    The cytotoxicity and non-specific cellular uptake of the most popular composition of upconversion nanoparticle (UCNP), NaYF4:Yb3+:Er3+, is reported using normal human skin cells, including dermal fibroblasts and immortalized human epidermal linear keratinocytes (HaCaT). A new hydrophilization reaction of as-synthesized UCNPs based on tetramethylammonium hydroxide (TMAH) enabled evaluation of the intrinsic cytotoxicity of bare UCNPs. The cytotoxicity effects of the UCNP surface-coating and polystyrene host were investigated over the concentration range 62.5–125 μg/mL with 24-h incubation, using a MTT test and optical microscopy. The fibroblast viability was not compromised by UCNPs, whereas the viability of keratinocytes varied from 52% ± 4% to 100% ± 10% than the control group, depending on the surface modification. Bare UCNPs reduced the keratinocyte viability to 76% ± 3%, while exhibiting profound non-specific cellular uptake. Hydrophilic poly(D,L-lactide)- and poly(maleic anhydride-alt-1-octadecene)-coated UCNPs were found to be least cytotoxic among the polymer-coated UCNPs, and were readily internalized by human skin cells. Polystyrene microbeads impregnated with UCNPs remained nontoxic. Surprisingly, no correlation was found between UCNP cytotoxicity and the internalization level in cells, although the latter ranged broadly from 0.03% to 59%, benchmarked against 100% uptake level of TMAH-UCNPs.

  3. Zubov V.P., Kapustin D.V., Generalova A.N., Yagudaeva E.Y.u., Vikhrov A.A., Sizova S.V., Muidinov M.R. (2007). Modification of solids with polymer nanolayers as a process for manufacture of novel biomaterial. POLYMER SCIENCE SERIES A 49 (12), 1247–1264 [+]

    The results of study on the chemical deposition of polymeric coatings of a nanoscale thickness on porous and flat inorganic matrices and encapsulation of nano-and microparticles in polymer shells are discussed. Procedures for the deposition of homogeneous defect-free coatings are detailed by using polytetrafluoroethylene, polyaniline, and their derivatives as examples. The matrices modified with nanosized polytetrafluoroethylene and polyaniline layers are promising biomaterials for one-step isolation of nucleic acids from complex biological mixtures (cell and tissue lysates, whole blood, plant feedstock), as well as for high-performance chromatography of proteins and other biopolymers. Approaches to the fabrication of polymer shells on luminescent nanocrystals of (CdSe)ZnS via the inclusion of the nanocrystals in micrometer-sized particles based on acrolein-styrene copolymers and the formation of polymer shells directly on nanoparticles are discussed. It was shown that polymer-functionalized luminescent nanocrystals hold promise as bioanalytical reagents.

  4. Kapustin D.V., Vikhrov A.A., Gorokhova I.V., Generalova A.N., Kalyazina O.V., Murzabekova T.V., Zubov V.P. (2005). Multicomponent thermosensitive systems for biocatalysts. RUSSIAN CHEMICAL BULLETIN 54 (2), 452–457 [+]

    Composite matrices based on macroporous silica modified by N-vinylcaprolactam copolymers with diallyldimethylammonium chloride and with 2-hydroxyethyl methacrylate were obtained. Lipase from Pseudomonas fluorescens was immobilized on the obtained materials. The temperature dependence of the hydrolytic activity of the immobilized lipase preparations in the triacetin hydrolysis was investigated. The hydrolytic activity of lipase immobilized on the matrix modified by the N-vinylcaprolactam copolymer with 2-hydroxyethyl methacrylate can be regulated by varying the temperature of the reaction medium. The temperature dependence of the hydrolytic activity of the immobilized enzyme has a maximum at 40 °C, the activity of the immobilized lipase being ∼3.5 times higher compared to that at 20 °C. After immobilization on these composite materials, lipase retained the activity in the acetylation of 1-(RS)-phenylethanol with vinyl acetate in ButOMe.