Laboratory of molecular theranostics
|Vladimir Martynov, D.Sc||Head of email@example.com, |
|Alexey Pakhomov, Ph.D.||s. r. firstname.lastname@example.org, |
|Alexey Garkovenko||j. r. email@example.com, |
|Anastasia Frolova||PhD firstname.lastname@example.org|
Key factors contributing to the green-to-red fluorescent protein transformation were identified
In collaboration with Laboratory of biomolecular modeling,  Group of in silico analysis of membrane proteins structure
Through the examples of two highly homologous fluorescent proteins from Zoanthus sp. (zoanGFP and zoan2RFP), amino acid residues participating in the transformation of a protein with the green fluorescence (GFP) into the red fluorescent protein (RFP) were explored. As the result of zoanGFP mutagenesis, internal amino acid residues (a.a.r.) became identical to those of zoan2RFP. However, this mutant underwent only partial transformation into the red form. To elucidate the extra factors that might affect red chromophore biosynthesis, we used comparative molecular dynamics simulations of zoan2RFP and zoanGFPmut. As the result, additional a.a.r. were discovered on the surface of the protein that might influence both the arrangement and flexibility of the chromophore-surrounding a.a.r. Site-directed mutagenesis of these external a.a.r. confirmed the crucial role of these residues in red chromophore biosynthesis.
- (2020). Impact of external amino acids on fluorescent protein chromophore biosynthesis revealed by molecular dynamics and mutagenesis studies. J Photochem Photobiol B 206, 111853
Fluorogenic marker for instant live-cell membrane staining and imaging
In collaboration with Group of growth factors expression and differentiation,  Group of Molecular Physiology
A new organic-compound-based fluorogenic marker has been created for live-cell membrane staining. Unlike current commercial cell markers, the obtained fluorogenic marker does not fluoresce in the aquatic environment, but acquires fluorescence immediately after being placed in a nonpolar medium, for example, in the cell membrane. This property allows one to instantly stain cells without further washing out the unbound dye. This marker can be applied in fluorescence microscopy for live-cell imaging and flow cytometry.
- (2017). BODIPY-based dye for no-wash live-cell staining and imaging. Biotechniques 63 (2), 77–79