BIOCHIM BIOPHYS ACTA, 2018, 1862(6):1410-1420

Structural basis of the signal transduction via transmembrane domain of the human growth hormone receptor

Background: Prior studies of the human growth hormone receptor (GHR) revealed a distinct role of spatial rearrangements of its dimeric transmembrane domain in signal transduction across membrane. Detailed structural information obtained in the present study allowed elucidating the bases of such rearrangement and provided novel insights into receptor functioning. Methods: We investigated the dimerization of recombinant TMD fragment GHR254–294by means of high-resolution NMR in DPC micelles and molecular dynamics in explicit POPC membrane. Results: We resolved two distinct dimeric structures of GHR TMD coexisting in membrane-