Phys Chem Chem Phys, 2016, 18(38):26703-26711

PH-Sensitive fluorophores from locked GFP chromophores by a non-alternant analogue of the photochemical: Meta effect

We report the synthesis and characterization of a pH-sensitive fluorescence switch based on a conformationally-locked green fluorescent protein (GFP) chromophore. The chromophore differs from difluoroboryl-locked parent by the addition of a titratable alcohol group on the imidazolinone ring. The chromophore is fluorescent at pH ≤ 5, but becomes non-fluorescent at higher pH, where the substituent is ionized. We use a quantum chemical model to show that the mechanism of the fluorescence turn-off is electronically analogous to photochemical meta effects in aryl-containing systems.

Olsen S, Baranov MS, Baleeva NS, Antonova MM, Johnson KA, Solntsev KM

IBCH: 3720
Ссылка на статью в журнале: http://xlink.rsc.org/?DOI=C6CP02423H
Кол-во цитирований на 09.2023: 9
Данные статьи проверены модераторами 2016-01-06