Moscow University Chemistry Bulletin, 2014, 69(2):49-55

Synthesis and application of firefly luciferase antibody conjugates in a bioluminescent immunoassay of Salmonella cells

A method has been developed for synthesizing stable and highly active conjugates of firefly luciferase with antibodies using the heterobifunctional coupling agent N-succinimidyl 3-(2-pyridyldithio) propionate linking NH2groups of antibodies with free SH groups of thermostable Luciola mingrelica firefly luciferase (Luc). A novel specific matrix for sorption of Salmonella cells has been designed that includes monodisperse polystyrene microparticles (φ = 240 nm) coated with Pluronic F108-PDS, which are then covalently linked to monoclonal antibodies against Salmonella cells (Sal). The Luc-Sal conjugates are used as detecting agents for bioluminescent immunoassay of Salmonella cells. The use of the novel matrix and highly active conjugates allow for a ~100-fold increase in the method's sensitivity compared with the standard sandwich assay. © 2014 Allerton Press, Inc.

Lomakina GY, Istrate A, Rudenko NV, Ugarova NN

IBCH: 4189
Ссылка на статью в журнале: http://link.springer.com/10.3103/S0027131414020047
Кол-во цитирований на 02.2024: 5
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