SVA elements represent the youngest family of hominid non-LTR retrotransposons. Recently, a human-specific subfamily (termed SVAF1, CpG-SVA, or MAST2-SVA) was discovered representing fusion of the CpG island-containing exon 1 of the MAST2 gene and a 5'-truncated SVA. SVAF1includes at least 84 members, which suggests exceptionally high retrotransposition level. We investigated if the acquirement of the MAST2 CpG-island might play a role in the success of the SVAF1subfamily. We observed that in 16 samples representing seven human tissues, MAST2 was cotranscribed with the members of the SVAF1subfamily, but not with other retrotransposons. We found that the methylation status of the MAST2-derived sequences of SVAF1elements reversely correlates with the transcriptional activity of MAST2. The MAST2 sequence at the 5' end of SVAF1acts as a positive transcriptional regulator in human germ cells. Finally, in various testicular tissue samples we uncovered a transcriptional correlation of MAST2 with the human L1, Alu and SVA retrotransposons. © 2012 Elsevier B.V.