Using DNA hybridization on cosmid filters of high density, we established chromosomal localization of the rpb9+gene encoding one of the specific subunits of RNA polymerase II of Schizosaccharomyces pombe and thus filled in the last gap in the mapping of the genes encoding components of RNA polymerase II of the fission yeast. The primary structure of three extended regions of the Sz. pombe chromosome I was elucidated and, as a result, genes neighboring on rpb9+were identified. One of them proved to be the tfa1+gene, encoding the large (α) subunit of the general factor of transcription initiation TFIIE. © 2000 MAIK "Nauka/Interperiodica".