Russ. J. Bioorganic Chem., 1996, 22(10):705-707
Kinetics of oxidation of ethanolamides of arachidonic (anandamide) and eicosapentaenoic acids by soybean 15-lipoxygenase
Oxidation of arachidonic acid ethanolamide (anandamide) and eicosapentaenoic acid ethanolamide by soybean 15-lipoxygenase was studied by UV spectroscopy. The KM values for both amides (3.2 and 4.9 μM, respectively) proved to be similar to that for arachidonic acid. Products of double lipoxygenase oxidation were identified in the reaction mixture at an enzyme-substrate ratio of 500:1. © 1996 MAEe Cyrillic signK Hayκa/Interperiodica Publishing.
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