Methods Mol Biol, 2019, 1973:281-297

Oligonucleotide Primers with G8AE-Clamp Modifications for RT-qPCR Detection of the Low-Copy dsRNA

We developed a new technique suitable for improved detection of low-copy dsRNA using modified oligonucleotides as primers in RT-qPCR. Insertion of G8AE-clamp residues into primers significantly improves thermal stability of duplexes with RNA without decrease of hybridization selectivity. The applicability of modified primers is demonstrated for detection of low-copy Kemerovo virus dsRNA.

Zatsepin TS, Varizhuk AM, Dedkov VG, Shipulin GA, Aralov AV

IBCH: 7915
Ссылка на статью в журнале: http://link.springer.com/10.1007/978-1-4939-9216-4_18
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Данные статьи проверены модераторами 2019-04-26