Sci Adv, 2020, 6(26):eaaz9861

A kinase bioscavenger provides antibiotic resistance by extremely tight substrate binding.

Microbial communities are self-controlled by repertoires of lethal agents, the antibiotics. In their turn, these antibiotics are regulated by bioscavengers that are selected in the course of evolution. Kinase-mediated phosphorylation represents one of the general strategies for the emergence of antibiotic resistance. A new subfamily of AmiN-like kinases, isolated from the Siberian bear microbiome, inactivates antibiotic amicoumacin by phosphorylation. The nanomolar substrate affinity defines AmiN as a phosphotransferase with a unique catalytic efficiency proximal to the diffusion limit. Crystallographic analysis and multiscale simulations revealed a catalytically perfect mechanism providing phosphorylation exclusively in the case of a closed active site that counteracts substrate promiscuity. AmiN kinase is a member of the previously unknown subfamily representing the first evidence of a specialized phosphotransferase bioscavenger.

Terekhov SS, Mokrushina YA, Nazarov AS, Zlobin A, Zalevsky A, Bourenkov G, Golovin A, Belogurov A, Osterman IA, Kulikova AA, Mitkevich VA, Lou HJ, Turk BE, Wilmanns M, Smirnov IV, Altman S, Gabibov AG

IBCH: 8666
Ссылка на статью в журнале: https://advances.sciencemag.org/lookup/doi/10.1126/sciadv.aaz9861
Кол-во цитирований на 12.2020: 3
Данные статьи проверены модераторами 2020-07-14

Список научных проектов, где отмечена публикация

  1. - (January 6, 2019 — December 31, 2020). Terekhov S.S.. Grant, RFBR.
  2. - (August 7, 2018 — December 7, 2021). Smirnov I.V.. Grant, RFBR.