Biochemistry (Mosc), 2006, 71(5):513-519
Renaturation, activation, and practical use of recombinant duplex-specific nuclease from Kamchatka crab
We overexpressed duplex-specific nuclease (DSN) from Kamchatka crab in Escherichia coli cells and developed procedures for purification, renaturation, and activation of this protein. We demonstrated identity of the properties of the native and recombinant DSN. We also successfully applied the recombinant DSN for full-length cDNA library normalization. © Pleiades Publishing, Inc., 2006.
Scopus: 2-s2.0-33744549226
: 16732729
IBCH: 914
Ссылка на статью в журнале: http://link.springer.com/10.1134/S0006297906050075
Кол-во цитирований на 01.2024: 17
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