Selected publications

1. Shcherbo D., Merzlyak E.M., Chepurnykh T.V., Fradkov A.F., Ermakova G.V., Solovieva E.A., Lukyanov K.A., Bogdanova E.A., Zaraisky A.G., Lukyanov S., Chudakov D.M. (2007). Bright far-red fluorescent protein for whole-body imaging. Nat. Methods 4 (9), 741–6 [+]

   A novel fluorescent protein Katushka with far-red emission preferable for signal registration inside animal tissues was created. Katushka is 10 fold brighter than other far-red proteins and is also characterized with fast maturation, high pH-stability and photostability. This constellation of properties makes it an instrument of choice for in vivo labeling of particular cells within whole organisms. A monomeric variant of Katushka named mKate was introduced for intracellular protein localization studies.

2. Merzlyak E.M., Goedhart J., Shcherbo D., Bulina M.E., Shcheglov A.S., Fradkov A.F., Gaintzeva A., Lukyanov K.A., Lukyanov S., Gadella T.W., Chudakov D.M. (2007). Bright monomeric red fluorescent protein with an extended fluorescence lifetime. Nat. Methods 4 (7), 555–7 [+]

   Fluorescent proteins have become extremely popular tools for in vivo imaging and especially for the study of localization, motility and interaction of proteins in living cells. Here we report TagRFP, a monomeric red fluorescent protein, which is characterized by high brightness, complete chromophore maturation, prolonged fluorescence lifetime and high pH-stability. These properties make TagRFP an excellent tag for protein localization studies and fluorescence resonance energy transfer (FRET) applications.

3. Belousov V.V., Fradkov A.F., Lukyanov K.A., Staroverov D.B., Shakhbazov K.S., Terskikh A.V., Lukyanov S. (2006). Genetically encoded fluorescent indicator for intracellular hydrogen peroxide. Nat. Methods 3 (4), 281–6 [+]

   A unique fluorescent sensor HyPer was introduced for in vivo monitoring of concentration of hydrogen peroxide — one of the major regulators of biological processes. Being a protein, HyPer can be expressed in cells or targeted specifically to a particular cell compartment. Due to its high specificity and sensitivity HyPer can be used for monitoring fluctuations of hydrogen peroxide concentration in a single cell or cell organelle.

4. Gurskaya N.G., Verkhusha V.V., Shcheglov A.S., Staroverov D.B., Chepurnykh T.V., Fradkov A.F., Lukyanov S., Lukyanov K.A. (2006). Engineering of a monomeric green-to-red photoactivatable fluorescent protein induced by blue light. Nat. Biotechnol. 24 (4), 461–5 [+]

   A novel monomeric fluorescent protein Dendra was developed, which is capable of irreversible photoconversion from a green fluorescent form into a red fluorescent one. Dendra is bright and can be activated with either UV or blue light.

5. Lukyanov K.A., Chudakov D.M., Fradkov A.F., Labas Y.A., Matz M.V., Lukyanov S. (2006). Discovery and properties of GFP-like proteins from nonbioluminescent anthozoa. Methods Biochem Anal 47, 121–38
6. Terskikh A., Fradkov A., Ermakova G., Zaraisky A., Tan P., Kajava A.V., Zhao X., Lukyanov S., Matz M., Kim S., Weissman I., Siebert P. (2000). "Fluorescent timer": protein that changes color with time. Science 290 (5496), 1585–8 [+]

   We generated a mutant of the red fluorescent protein drFP583. The mutant (E5) changes its fluorescence from green to red over time. The rate of color conversion is independent of protein concentration and therefore can be used to trace time-dependent expression. We used in vivo labeling with E5 to measure expression from the heat shock-dependent promoter in Caenorhabditis elegans and from the Otx-2 promoter in developing Xenopus embryos. Thus, E5 is a "fluorescent timer" that can be used to monitor both activation and down-regulation of target promoters on the whole-organism scale.

7. Matz M.V., Fradkov A.F., Labas Y.A., Savitsky A.P., Zaraisky A.G., Markelov M.L., Lukyanov S.A. (1999). Fluorescent proteins from nonbioluminescent Anthozoa species. Nat. Biotechnol. 17 (10), 969–73 [+]

   Novel fluorescent proteins with different fluorescence colors from blue to red were found in Anthozoa species. Discovery of chromo- and fluorescent GFP-like proteins in non-bioluminescent coral polyps disproved the common belief, that these proteins are obligatory attached to bioluminescense systems and disclosed the nature of fluorescent coloration of corals — a phenomenon, that didn’t have proper explanation before.