Laboratory of Extrasynaptic Signalling

Department of Molecular Neurobiology

Head: Alexey Semyanov, corresponding member of the academy of sciences
semyanov@ibch.ru

neurons, glia, brain

The laboratory is engaged in research of cellular and molecular mechanisms of information coding in neurons and glia networks, which are the basis of learning and memory.

Researchers are solving the following tasks:
  • To study how internal factors (hormones, pregnancy, aging) and environmental factors (anthropogenic pollution, nutrition, microbiota) influence on the brain functions.
  • To study of neurodegenerative diseases, such as epilepsy, Alzheimer's disease, lateral amyotrophic sclerosis, Parkinson's disease.
  • Mathematical modeling of processes in the brain.
  1. Cell technologies. Studies using electrophysiological and optical methods of processes occurring in neuron-astrocytic cultures derived from animals or from human iPS cells.
  2. Work with brain sections. The use of electrophysiological (patch-clamp) and optical methods – two-photon laser scanning microscopy, FLIM, superresolution.
  3. In vivo studies. Optical imaging and electrophysiology in vivo, behavior.
NamePositionContacts
Alexey Brazhes. r. f.
Maxim Doroninj. r. f.doronin@ibch.ru
Alexander Popovj. r. f.
Alexey Semyanov, corresponding member of the academy of sciencesHead of lab.semyanov@ibch.ru

Selected publications

  1. Gavrilov N, Golyagina I, Brazhe A, Scimemi A, Turlapov V, Semyanov A (2018). Astrocytic coverage of dendritic spines, dendritic shafts, and axonal boutons in hippocampal neuropil. Front Cell Neurosci 12 (0), 248
  2. Simanovskaya AA, Ivashina TV, Zeifman YS, Fateeva TV, Krukova MV, Popov AN, Kachalova GS, Rakitina TV (2018). Mutation L232H Promotes Chromophore Maturation of EGFP-Based Fluorescent Fusion Proteins. CRYSTALLOGR REP+ 63 (2), 191–195
  3. Lebedeva A, Plata A, Nosova O, Tyurikova O, Semyanov A (2018). Activity-dependent changes in transporter and potassium currents in hippocampal astrocytes. Brain Res Bull 136 (0), 37–43
  4. Jennings A, Tyurikova O, Bard L, Zheng K, Semyanov A, Henneberger C, Rusakov DA (2017). Dopamine elevates and lowers astroglial Ca2+through distinct pathways depending on local synaptic circuitry. Glia 65 (3), 447–459
  5. Snezhkina AV, Krasnov GS, Zaretsky AR, Zhavoronkov A, Nyushko KM, Moskalev AA, Karpova IY, Afremova AI, Lipatova AV, Kochetkov DV, Fedorova MS, Volchenko NN, Sadritdinova AF, Melnikova NV, Sidorov DV, Popov AY, Kalinin DV, Kaprin AD, Alekseev BY, Dmitriev AA, Kudryavtseva AV (2016). Differential expression of alternatively spliced transcripts related to energy metabolism in colorectal cancer. BMC Genomics 17 (0), 1011
  6. Vedunova MV, Mishchenko TA, Mitroshina EV, Ponomareva NV, Yudintsev AV, Generalova AN, Deyev SM, Mukhina IV, Semyanov AV, Zvyagin AV (2016). Cytotoxic effects of upconversion nanoparticles in primary hippocampal cultures. RSC Adv 6 (40), 33656–33665
  7. Pavlov I, Savtchenko LP, Song I, Koo J, Pimashkin A, Rusakov DA, Semyanov A (2014). Tonic GABAAconductance bidirectionally controls interneuron firing pattern and synchronization in the CA3 hippocampal network. Proc Natl Acad Sci U S A 111 (1), 504–509
  8. Shih PY, Savtchenko LP, Kamasawa N, Dembitskaya Y, McHugh TJ, Rusakov DA, Shigemoto R, Semyanov A (2013). Retrograde Synaptic Signaling Mediated by K+ Efflux through Postsynaptic NMDA Receptors. Cell Rep 5 (4), 941–951
  9. Gushchin I, Chervakov P, Kuzmichev P, Popov AN, Round E, Borshchevskiy V, Ishchenko A, Petrovskaya L, Chupin V, Dolgikh DA, Arseniev AA, Kirpichnikov M, Gordeliy V (2013). Structural insights into the proton pumping by unusual proteorhodopsin from nonmarine bacteria. Proc Natl Acad Sci U S A 110 (31), 12631–12636
  10. Grebenik EA, Nadort A, Generalova AN, Nechaev AV, Sreenivasan VKA, Khaydukov EV, Semchishen VA, Popov AP, Sokolov VI, Akhmanov AS, Zubov VP, Klinov DV, Panchenko VY, Deyev SM, Zvyagin AV (2013). Feasibility study of the optical imaging of a breast cancer lesion labeled with upconversion nanoparticle biocomplexes. J Biomed Opt 18 (7), 76004
  11. Samygina VR, Ochoa-Lizarralde B, Popov AN, Cabo-Bilbao A, Goni-De-Cerio F, Molotkovsky JG, Patel DJ, Brown RE, Malinina L (2013). Structural insights into lipid-dependent reversible dimerization of human GLTP. Acta Crystallogr D Biol Crystallogr 69 (4), 603–616
  12. Wlodarczyk AI, Sylantyev S, Herd MB, Kersanté F, Lambert JJ, Rusakov DA, Linthorst ACE, Semyanov A, Belelli D, Pavlov I, Walker MC (2013). GABA-independent GABAAreceptor openings maintain tonic currents. J Neurosci 33 (9), 3905–3914
  13. Wu YW, Grebenyuk S, McHugh TJ, Rusakov DA, Semyanov A (2012). Backpropagating Action Potentials Enable Detection of Extrasynaptic Glutamate by NMDA Receptors. Cell Rep 1 (5), 495–505
  14. Samygina VR, Popov AN, Cabo-Bilbao A, Ochoa-Lizarralde B, Goni-De-Cerio F, Zhai X, Molotkovsky JG, Patel DJ, Brown RE, Malinina L (2011). Enhanced selectivity for sulfatide by engineered human glycolipid transfer protein. Structure 19 (11), 1644–1654
  15. Song I, Savtchenko L, Semyanov A (2011). Tonic excitation or inhibition is set by GABAAconductance in hippocampal interneurons. Nat Commun 2 (1), 376
  16. Kochlamazashvili G, Senkov O, Grebenyuk S, Robinson C, Xiao MF, Stummeyer K, Gerardy-Schahn R, Engel AK, Feig L, Semyanov A, Suppiramaniam V, Schachner M, Dityatev A (2010). Neural cell adhesion molecule-associated polysialic acid regulates synaptic plasticity and learning by restraining the signaling through GluN2B-containing NMDA receptors. J Neurosci 30 (11), 4171–4183
  17. Pavlov I, Savtchenko LP, Kullmann DM, Semyanov A, Walker MC (2009). Outwardly rectifying tonically active GABAA receptors in pyramidal cells modulate neuronal offset, not gain. J Neurosci 29 (48), 15341–15350
  18. Wanaverbecq N, Semyanov A, Pavlov I, Walker MC, Kullmann DM (2007). Cholinergic axons modulate GABAergic signaling among hippocampal interneurons via postsynaptic α7 nicotinic receptors. J Neurosci 27 (21), 5683–5693
  19. Scimemi A, Semyanov A, Sperk G, Kullmann DM, Walker MC (2005). Multiple and plastic receptors mediate tonic GABAA receptor currents in the hippocampus. J Neurosci 25 (43), 10016–10024
  20. Semyanov A, Walker MC, Kullmann DM, Silver RA (2004). Tonically active GABAAreceptors: Modulating gain and maintaining the tone. Trends Neurosci 27 (5), 262–269
  21. Semyanov A, Walker MC, Kullmann DM (2003). GABA uptake regulates cortical excitability via cell type-specific tonic inhibition. Nat Neurosci 6 (5), 484–490
  22. Semyanov A, Kullmann DM (2001). Kainate receptor-dependent axonal depolarization and action potential initiation in interneurons. Nat Neurosci 4 (7), 718–723
  23. Semyanov A, Kullmann DM (2000). Modulation of GABAergic signaling among interneurons by metabotropic glutamate receptors. Neuron 25 (3), 663–672
  24. PERVUSHIN KV, OREKHOV VY, POPOV AI, MUSINA LY, ARSENIEV AS (1994). Three‐dimensional structure of (1–71)bacterioopsin solubilized in methanol/chloroform and SDS micelles determined by15N‐1H heteronuclear NMR spectroscopy. FEBS J 219 (12), 571–583

Alexey Semyanov