Laboratory of Molecular Biophysics
|Vladimir Oleinikov, D.Sc||depart. firstname.lastname@example.org, |
|Konstantin Mochalov, Ph.D.||s. r. email@example.com, |
|Svetlana Sizova, Ph.D.||r. firstname.lastname@example.org|
|Yury Kozmin||r. email@example.com, |
|Marina Tretyak||r. firstname.lastname@example.org, |
|Daria Solovyeva||j. r. email@example.com, |
|Tatiana Sukhanova||j. r. firstname.lastname@example.org, |
|Anton Zalygin||j. r. f.|
|Vasiliy Koval||PhD email@example.com|
|Veronika Manohina||res. eng.|
|Aleksej Mezin||res. eng.|
|Anastasia Gileva||res. firstname.lastname@example.org|
|Anton Efimov||s. r. f.|
Supramers on the base of amphiphillic molecules lipid-oligopeptide-biotin (2018-11-28)
It was found that oligopeptides with terminal lipid and biotin fragments are able to form micelle-like supramers (globules) in an aqueous solution. Using optical spectroscopy, atomic-force and electron microscopy, as well as small-angle X-ray scattering and computer simulation, it was shown that the globules are very uniform in size (about 14.6 nm). It was found that globules have the core/shell structure. The core contains lipid and part (up to 90%) of the biotin fragments. The polar oligopeptide spacer folds back upon itself and predominantly places the biotin reside inside the globule. But the part ( <10%) of biotin residues is exposed outside, and can be used for the selective attachment of specified molecules. Micelle-like supramers containing compounds that are natural to a living organism can become the basis for new types of carriers for targeted drug delivery.
High-Performance Bio-Photoelectrodes for Quantification and Measurements of Photocurrent Generated by a Single Photosynthetic Reaction Center (2018-11-28)
A new approach to fabrication of a high-performance biophotoelectrodes consisting of photosynthetic reaction center (RC) proteins immobilized on a conducting surface is developed. The voltammetry measurements at the conductive interface between a single photosynthetic RC and a golden electrode were used as the base of this study. It allowed use not only the quantification of the efficiency of the method of RC immobilization used, but also the measurements of current of the single RC. It was shown that covalent RC immobilization through S-Au bonds is much more efficient (up to ten times) than the use of a spacer. Development of this approach can be very perspective for creation of highly efficient biohybrid photosensing devices.
A hardware for the study of hybrid (polariton) states "organic molecule - photon microresonator" (2018-11-28)
A hardware-methodical module for the study of hybrid (polariton) states "organic molecule - photon microresonator" has been developed for studying the modulation of the rate of biochemical reactions under conditions of a strong "light-substance" relationship.
The resonant coupling between excited states of a matter with optical resonator eigenmodes the results new hybrid energy states are formed, which can be described using the corresponding quasiparticles - polaritons. The properties of polaritons are significantly different from the properties of both the photon and the excited state in matter. Of particular interest is the strong coupling regime, in which the rate of energy exchange between light and matter exceeds the energy loss in the system, which leads to the formation of two new hybrid energy states of the system - Rabi splitting. In this mode, the fundamental properties of the matter such as the spectral and temporal characteristics of the excited states may be changed that can be used to enhance of Raman scattering, resonant nonradiative energy transfer over long distances and modify the speed of chemical reactions. We have developed a universal device based on a tunable unstable Fabry-Perot microcavity combining the following advantages: versatility, small mode volume, and the possibility of smoothly changing the quality factor and mode volume in the process of working with one sample. A series of fluorescent dyes encapsulated in polymer matrices was investigated. For the first time, significant values of Rabi splitting (225 meV) were obtained for low concentrations of undirected rhodamine 6G molecules with a wide photoluminescence band.
- (2018). A versatile tunable microcavity for investigation of light-matter interaction. Rev Sci Instrum 89 (5), 053105
Unique scientific setup “System for probe-optical 3D correlative microscopy” (2017-11-28)
Designed, build and included in the register of unique scientific experimental instruments “System for probe-optical 3D correlative microscopy”
The new tool is designed to obtain three-dimensional images of biological and other objects, reconstruction of the 3D distribution of various physicochemical parameters along the volume of the object, including the determination of the chemical composition in the local regions of the object with a resolution of the nanometer scale.
All techniques of SPM are available (contact, semicontact, dynamic contact, and tapping modes, force modulation, magnetic and electrostatic force, Kelvin-probe and scanning spreading resistance microscopy. Also, it has the possibility to use the confocal fluorescence, Raman, surface and tip enhanced Raman scattering spectroscopy (SERS and TERS), and SNOM. Axial resolution of ultramicrotomography – 20 nm. Instrument contains the hard and software for reconstruction 3-D reconstruction of the morphological/optical structure of samples.
The figure shows the appearance of a unique tool, its scheme and principle of action. As the example of application, the "architecture" of the magnetic-fluorescent microbeads for clinical diagnosis and drug delivery, determined using ultramicrotomography, fluorescent microspectroscopy, and magnetic force microscopy was defined.
- (2017). An instrumental approach to combining confocal microspectroscopy and 3D scanning probe nanotomography. Ultramicroscopy 182, 118–123
- (2017). A novel design of a scanning probe microscope integrated with an ultramicrotome for serial block-face nanotomography. Rev Sci Instrum 88 (2), 023701