Group of Molecular Ecology

Olga Trubetskaya,
Valentina Sokolovaeng.

All publications (show selected)


Olga Trubetskaya

  • Russia, Moscow, Ul. Miklukho-Maklaya 16/10 — On the map
  • IBCh RAS, build. ФИБХ, office. Переход/438
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On the way to obtaining environmentally friendly photodestructors of organic pollutants based on humic substances

Chernozem humic acid (HA) fractions with nominal molecular sizes (MS) >100, 30–100, 5–30 and <5 kDa were obtained, using a combination of low-pressure preparative size exclusion chromatography with analytical polyacrylamide gel electrophoresis or multistep ultrafiltration. The initial HA and fractions were tested for the ability to destroy model aromatic compounds 2,4,6-trimethylphenol and furfurol in aqueous solution when illuminated with polychromatic light in the range 300–450 nm under laboratory conditions.

It has been found that chernozem HA fractions with MS less than 5 kDa had the maximum ability to decompose the above-mentioned substances, while fractions with MS above 30 kDa practically did not perform this function. The data obtained allow us to explain partially the mechanisms of the photoinduced transformation of many organic pollutants by soil humic substances.


  1. Trubetskoi OA, Patsaeva SV, Trubetskaya OE (2019). Photochemical Degradation of Organic Pollutants in Solutions of Soil Humic Acids. EURASIAN SOIL SCI+ 52 (9), 1075–1080

Identification of fluorophores in nanoparticles of dissolved organic matter from the Suwanee River (USA)

Analytical high performance size exclusion liquid chromatography with multiwavelength absorbance and fluorescence detections was used for the analysis of molecular size (MS) distribution and optical properties of dissolved natural organic matter (NOM). Experiments were conducted on Suwannee River organic matter (SRNOM) nanoparticles and its different on MS fractions preliminary obtained by combination of preparative low pressure size exclusion chromatography and polyacrylamide gel electrophoresis. The multistep fractionation of SRNOM enabled the size separation of at least five types of humic-like fluorophores within NOM showing emission maxima at 465, 450, 435, 420, and 405 nm. This work provides new data on the optical properties of size-fractionated aquatic NOM, which consistent with the formation of supramolecular NOM assemblies, likely controlled by association of low-molecular size components.

3-D fluorescence spectra as a test system for evaluation the efficiency of humic substances preparative fractionation.

In recent years, three-dimensional fluorescence spectroscopy has been widely used to study complex heterogeneous mixtures, including natural humic substances, based on computer analysis of data obtained after removing several tens of emission spectra of the same sample at different excitation wavelengths of a given range. The set of data allows to obtain three-dimensional fluorescent diagrams with identification of all possible fluorescence maxima and their intensities. This method has been mastered and successfully used as a test system for evaluating the efficiency of fractionation of humic substances of various origin for the purpose of isolating and identifying individual fluorophores. The figure shows the three-dimensional fluorescent diagrams of the initial preparation of the dissolved organic matter and its fractions obtained by preparative exclusion chromatography. The three-dimensional fluorescent diagram allows not only to estimate the efficiency of fractionation, but also serve as a direct confirmation of the supramolecular organization of natural humic substances.Publications:Trubetskoi O.A., Trubetskaya O.E. Three-dimensional fluorescence analysis of chernozem humic acids and their electrophoretic fractions. Eurasian Soil Science (2017) 50:1018-1024. (Перевод: Почвоведение 2017, № 9, с.1057-1064). DOI:10.7868/S0032180X17090088Trubetskoi O.A., Trubetskaya O.E. Identification of fluorophores of organic matter of natural water. Water Resources (2017) Accepted for publication.