Group of Molecular Ecology

Olga Trubetskaya, Ph.D.depart.
Valentina Sokolovapr. lab. as.

Selected publications (show all)


Olga Trubetskaya

  • Russia, Moscow, Ul. Miklukho-Maklaya 16/10 — On the map
  • IBCh RAS, build. ФИБХ, office. Переход/438
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Identification of fluorophores in nanoparticles of dissolved organic matter from the Suwanee River (USA) (2018-11-26)

Analytical high performance size exclusion liquid chromatography with multiwavelength absorbance and fluorescence detections was used for the analysis of molecular size (MS) distribution and optical properties of dissolved natural organic matter (NOM). Experiments were conducted on Suwannee River organic matter (SRNOM) nanoparticles and its different on MS fractions preliminary obtained by combination of preparative low pressure size exclusion chromatography and polyacrylamide gel electrophoresis. The multistep fractionation of SRNOM enabled the size separation of at least five types of humic-like fluorophores within NOM showing emission maxima at 465, 450, 435, 420, and 405 nm. This work provides new data on the optical properties of size-fractionated aquatic NOM, which consistent with the formation of supramolecular NOM assemblies, likely controlled by association of low-molecular size components.

3-D fluorescence spectra as a test system for evaluation the efficiency of humic substances preparative fractionation. (2017-11-28)

In recent years, three-dimensional fluorescence spectroscopy has been widely used to study complex heterogeneous mixtures, including natural humic substances, based on computer analysis of data obtained after removing several tens of emission spectra of the same sample at different excitation wavelengths of a given range. The set of data allows to obtain three-dimensional fluorescent diagrams with identification of all possible fluorescence maxima and their intensities. This method has been mastered and successfully used as a test system for evaluating the efficiency of fractionation of humic substances of various origin for the purpose of isolating and identifying individual fluorophores. The figure shows the three-dimensional fluorescent diagrams of the initial preparation of the dissolved organic matter and its fractions obtained by preparative exclusion chromatography. The three-dimensional fluorescent diagram allows not only to estimate the efficiency of fractionation, but also serve as a direct confirmation of the supramolecular organization of natural humic substances.Publications:Trubetskoi O.A., Trubetskaya O.E. Three-dimensional fluorescence analysis of chernozem humic acids and their electrophoretic fractions. Eurasian Soil Science (2017) 50:1018-1024. (Перевод: Почвоведение 2017, № 9, с.1057-1064). DOI:10.7868/S0032180X17090088Trubetskoi O.A., Trubetskaya O.E. Identification of fluorophores of organic matter of natural water. Water Resources (2017) Accepted for publication.