Synthesis and fluorescent characteristics of oligodeoxynucleotides containing a novel fluorescent label, p-(2-benzoxazolyl)tolane
Это дубликат статьи: «Synthesis and Fluorescent Characteristics of Oligodeoxynucleotides Containing a Novel Fluorescent Label, p-(2-Benzoxazolyl)tolane»
A functional (dihydroxybutyl) derivative of p-(2-benzoxazolyl)tolane, a fluorescent label novel for biopolymers, was synthesized. The functionalized solid support obtained on its basis was employed in the synthesis of oligodeoxynucleotides 3-terminally labeled with benzoxazolyltolane (these oligonucleotides also contained 1-phenylethynylpyrene residues). This fluorophore within its dihydroxybutyl derivative and the oligonucleotides modified with it displays an intensive fluorescence characterized by a high Stokes shift. The oligonucleotides labeled with this fluorophore are potential probes sensitive to the biopolymer microenvironment. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2001, vol. 27, no. 6; see also http://www.maik.ru.