Biotechniques, 2017, 63(2):77-79

BODIPY-based dye for no-wash live-cell staining and imaging

All rights reserved. In nonpolar solvents, hydrophobic organic fluorophores often show bright fluorescence, whereas in polar media, they usually suffer from aggregation-caused quenching (ACQ). Here, we harnessed this solvatochromic behavior of a 1,3,5,7-tetramethyl-BODIPY derivative for cell staining and applied it to live-cell imaging and flow cytometry. As opposed to commercially available dyes, this BODIPY derivative showed excellent contrast immediately after staining and did not require any wash-off.

Pakhomov AA, Deyev IE, Ratnikova NM, Chumakov SP, Mironiuk VB, Kononevich YN, Muzafarov AM, Martynov VI

IBCH: 3447
Ссылка на статью в журнале: https://www.future-science.com/doi/10.2144/000114577
Кол-во цитирований на 09.2020: 2
Данные статьи проверены модераторами 2017-01-06