Toxicon, 2015, 107(Pt B):197-209

Quantitative proteomic analysis of Vietnamese krait venoms: Neurotoxins are the major components in Bungarus multicinctus and phospholipases A2in Bungarus fasciatus

All rights reserved. Kraits are venomous snakes of genus Bungarus from family Elapidae. Krait venoms are generally neurotoxic, but toxicity strongly depends on the particular species and regional origin of snakes. We analyzed the proteomes of Vietnamese Bungarus multicinctus and Bungarus fasciatus venoms both qualitatively and quantitatively. It should be noted that no proteomic data for B. multicinctus venom existed so far. We have found that in this venom, almost half (45%) of the proteins by weight was represented by β-bungarotoxins, followed by three finger toxins (28%) and phospholipases A2(16%), other proteins being present at the level of 1-3%. In B. fasciatus venom, phospholipase A2was the main component (71%), followed by oxidase of l-amino acids (8%), acetylcholinesterase (5%) and metalloproteinases (4%). Unexpectedly, extremely low amount of three finger toxins (1%) was found in this venom. Interestingly, the presence of complement depleting factor was observed in both venoms. Although our data showed the presence of the same toxin families in Vietnamese krait venoms as those found earlier in the venoms of kraits from other geographic regions, their relative ratio is completely different. This concerns especially B. fasciatus venom with predominant content of phospholipases A2and very low amount of three finger toxins.

Ziganshin RH, Kovalchuk SI, Arapidi GP, Starkov VG, Hoang AN, Thi Nguyen TT, Nguyen KC, Shoibonov BB, Tsetlin VI, Utkin YN

IBCH: 3754
Ссылка на статью в журнале: https://linkinghub.elsevier.com/retrieve/pii/S0041010115300568
Кол-во цитирований на 05.2020: 26
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