J Immunol Res, 2017, 2017:4645701

Bactericide, immunomodulating, and wound healing properties of transgenic Kalanchoe pinnata synergize with antimicrobial peptide cecropin P1 in Vivo

Procedure ofmanufacturing K. pinnata water extracts containing cecropin P1 (CecP1) fromthe formerly described transgenic plants is established. It included incubation of leaves at +4°C for 7 days, mechanical homogenization of leaves using water as extraction solvent, and heating at +70°C for inactivating plant enzymes. Yield of CecP1 (after heating and sterilizing filtration) was 0.3% of total protein in the extract. The water extract of K. pinnata + CecP1 exhibits favorable effect on healing of wounds infected with S. aureus (equal to Cefazolin) and with a combination of S. aureus with P. aeruginosa (better than Cefazolin). Wild-type K. pinnata extract exhibited evident microbicide activity against S. aureus with P. aeruginosabut itwas substantially strengthened inK. pinnata+CecP1 extract. K. pinnata extracts (both wild-type and transgenic) did not exhibit general toxicity and accelerated wound recovery. Due to immunomodulating activity, wild-type K. pinnata extract accelerated granulation of the wound bed and marginal epithelialization even better than K. pinnata + CecP1 extract. Immunomodulating and microbicide activity of K. pinnata synergizes with microbicide activity of CecP1 accelerating elimination of bacteria.

Lebedeva AA, Zakharchenko NS, Trubnikova EV, Medvedeva OA, Kuznetsova TV, Masgutova GA, Zylkova MV, Buryanov YI, Belous AS

IBCH: 4034
Ссылка на статью в журнале: https://www.hindawi.com/journals/jir/2017/4645701/
Кол-во цитирований на 09.2020: 7
Данные статьи проверены модераторами 2017-02-06