Gene Expression of Antimicrobial Peptide Bombinin Increases Resistance of Tobacco Transgenic Plants to Phytopathogens
Transgenic tobacco (Nicotiana tabacum L.) plants with an artificial gene of an antimicrobial peptide bombinin (bom) have been obtained and investigated. The presence of the bom gene in the genome of kanamycin-resistant plants was shown by PCR. The bom gene expression was confirmed by the antimicrobial activity measurements in leaf extracts. The obtained plants were morphogenetically resistant to the Erwinia carotovora bacteria and Rhizoctonia solani fungi phytopathogens. In addition, in the transgenic plants, the protective oxidative reaction to the infection, namely the SOD activity and proline content, were lower than in the infected nontransgenic plants. The plants with the expression of the antimicrobial bombinin peptide gene are promising for the use in agricultural biotechnology as plant protectors.