Chemistry, 2019, 25(41):9592-9596

Red-shifted substrates for FAST fluorogen-activating protein based on the GFP-like chromophores

In this paper, we present a novel genetically encoded fluorescent tag for live cell microscopy. This tag is composed of previously published fluorogen-activating protein FAST and a novel fluorogenic derivative of GFP-like chromophore with red fluorescence. The reversible binding of the novel fluorogen and FAST is accompanied by three orders of magnitude increase in red fluorescence (580-650 nm). The proposed dye instantly stains target cellular proteins fused with FAST, washes out in a minute timescale, and exhibits higher photostability of the fluorescence signal in confocal and widefield microscopy, in contrast with previously published fluorogen:FAST complexes.

IBCH: 7957
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Кол-во цитирований на 09.2020: 10
Данные статьи проверены модераторами 2019-05-23