ACS Sens, 2020

Ultrafast, Ultrasensitive Detection and Imaging of Single Cardiac Troponin-T Molecules.

The fluorescence-based methods of single-molecule optical detection have opened up unprecedented possibilities for imaging, monitoring, and sensing at a single-molecule level. However, single-molecule detection methods are very slow, making them practically inapplicable. In this paper, we show how to overcome this key limitation using the expanded laser spot, laser excitation in a nonfluorescent spectral window of biomolecules, and more binding fluorescent molecules on a biomolecule that increases the detection volume and the number of collected photons. We demonstrate advantages of the developed approach unreachable by any other technique using detection of single cardiac troponin-T molecules: (i) 1000-fold faster than by known approaches, (ii) real-time imaging of single troponin-T molecules dissolved in human blood serum, (iii) measurement of troponin-T concentration with a clinically important sensitivity of about 1 pg/mL. The developed approach can be used for ultrafast, ultrasensitive detection, monitoring, and real-time imaging of other biomolecules as well as of larger objects including pathogenic viruses and bacteria.

Melentiev PN, Son LV, Kudryavtsev DS, Kasheverov IE, Tsetlin VI, Esenaliev RO, Balykin VI

IBCH: 8851
Ссылка на статью в журнале: https://pubs.acs.org/doi/10.1021/acssensors.0c01790
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Данные статьи проверены модераторами 2020-11-02