Artificial Climate Station “BIOTRON”

Department of Plant Molecular Biology and Biotechnology

Head: Sergey Dolgov, D.Sc
dolgov@bibch.ru+7(4967)73-06-53#3237, +7(3237)73-17-79#3126

The subdivision is one of the leading Russian laboratories, successfully carrying out research in various areas of plant biotechnology. The employees of the Laboratory face the task of obtaining priority scientific results in the field of molecular biology, the functioning and organization of the plant genome, the creation of biotech forms of new-generation plants for fundamental and applied research that are designed to ensure the development of Russia's agro-industrial complex and promote the development of the production of medical and veterinary substances using plant-producers (bio-factories).

For more than 20 years, the Laboratory has been conducting research on the development of agrobacterial and ballistic methods for the production of transgenic plants of fruit, berry, vegetable, cereals, ornamental crops with new economically valuable traits. During this time, the Laboratory staff received more than a thousand independent transgenic plants from such important agricultural crops as wheat, tomato, apple, pear, plum, carrot, beet, etc. Work is underway to clone plant genes and create modern expression systems and genetic structures for successful modification of genomes of cultivated plants. Since 2000, the Laboratory staff has been conducting field trials of transgenic plants, many of these studies were performed for the first time not only in Russia, but also in the world.

The laboratory operates on the basis of a unique scientific installation (USI) "Station for working out methods for obtaining transgenic plants and ballistic transformation of plant objects".

All publications (show selected)

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Sergey Dolgov

  • Russia, Moscow, Ul. Miklukho-Maklaya 16/10 — On the map
  • IBCh RAS, build. ФИБХ, office. Лаб-1/201-4
  • Phone: +7(4967)73-06-53#3237
  • E-mail: dolgov@bibch.ru

Production of marker-free tomato plants expressing the supersweet protein thaumatin II gene under the control of predominantly fruit-specific promoters

Despite the lack of evidence of the danger of genetically modified organisms the presence of marker and antibiotic-resistant genes in transgenic plants causes concern to consumers. Genetically modified plants with viral and bacterial genes are adopted by consumers, but with concerns; in addition, constitutive promoters have a number of disadvantages in industrial-scale cultivation of plants. In our study, we used the pMF vector system (Wageningen Plant Research, Wageningen, Netherlands), which combines inducible site-specific recombinase and a bifunctional selectable gene to obtain marker-free tomato plants. The gene of interest was the supersweet thaumatin II protein from the tropical plant Thaumatococcus daniellii under the control of tomato predominantly fruit-specific early-light inducible protein (ELIP) or E8 promoters and tomato Rubisco terminator. The use of this gene in our laboratory allowed enhancing sweetness, as well as improving the taste characteristics of fruit such as apple, strawberries, carrots, tomatoes, and pears. By using different strategies of early and delayed selection we developed a protocol for obtaining fully marker-free tomato plants, which was checked by polymerase chain reaction and Southern blotting. The thaumatin II gene expression was confirmed by reverse transcription-PCR and western blotting analyses. The fruit of transgenic and marker-free tomato plants displayed a sweet taste. A quantitative comparative assessment of the level of expression of the thaumatin protein under the control of two promoters was carried out using enzyme-linked immunosorbent assay. Multiple and/or incomplete T-DNA inserts that often occur during transformation of Solanaceae greatly reduced the efficiency of the system used.

The test on laboratory animals of the recombinant vaccine protein M2e-RTB, obtained in the duckweed plants.

In accordance with the research plan in 2018, a molecular-biological analysis of duckweed plants was carried out; the duckweed lines, producers of recombinant vaccine protein M2e-RTB, were obtained for the first time. The recombinant protein M2e-RTB obtained in plants of the duckweed was orally immunized to mice, and induction of antibodies to the peptide M2e of the avian influenza virus was shown.

"Development of systems for the synthesis of recombinant proteins based on plant expression platforms (biofarming)". № 01201352434

Study of molecular biological mechanisms and the development of molecular breeding methods for obtaining plants of agricultural crops that are resistant to environmental stress factors and with improved crop quality

An analysis of the inheritance of transgenic traits in the offspring of 54 primary transgenic lines of T0 common wheat, containing the genes for resistance to the herbicide bar and salt stress hvnxh2 or agnhx, was carried out. 165 T2-T3 populations inheriting stress resistance genes as homozygous alleles were identified. Molecular and biological analysis confirmed the expression of salt tolerance genes in the homozygous progeny of 16 T0 lines. To study the post-transcriptional regulation of the ripening time of fruits, 27 transgenic apple lines adapted to ex vitro conditions, which contain expression cassettes to suppress ethylene synthesis, were successfully grafted onto rootstocks in order to accelerate their flowering and fruiting.

Study of molecular biological mechanisms and the development of molecular breeding methods for obtaining plants of agricultural crops that are resistant to environmental stress factors and with improved crop quality. № 0101-2014-0047, № 01201352435

Mega-grant from the Government of the Russian Federation

In collaboration with Laboratory of Molecular Bases of Plant Stress Resistance

The Laboratory of Molecular Bases of Plant Stress Resistance was established in 2017 when Professor M. Taliansky was awarded a mega-grant from the Russian government on the topic "New functions of the cell nucleus and complex resistance of potato plants to diseases and physiological stresses". The activity of the laboratory is aimed at obtaining comprehensive knowledge of the complex stresses in plants mechanisms and identifying key target genes necessary to protect plants from the impact of various stresses on the growth, development and productivity of plants.