Кондратьева Лия Германовна


Период обученияСтрана, городУчебное заведениеДополнительная информация
2009–2014 Россия, Москва Московский Государственный Университет им. М. В. Ломоносова, Биологический Факультет, кафедра вирусологии Красный диплом, специальность биохимия

Гранты и проекты

ПериодДополнительная информация
2015–2015 Грант РФФИ 15-04-07773 А
2014–2018 Грант Российского научного фонда, проект № 14-50-00131

Избранные публикации

  1. Kondratyeva L.G., Sveshnikova A.A., Grankina E.V., Chernov I.P., Kopantseva M.R., Kopantzev E.P., Sverdlov E.D. (2016). Downregulation of expression of mater genes SOX9, FOXA2, and GATA4 in pancreatic cancer cells stimulated with TGFβ1 epithelial-mesenchymal transition. Dokl. Biochem. Biophys. 469 (1), 257–9 [+]

    We show characteristic morphological changes corresponding to epithelial-mesenchymal transition (EMT) program fulfillment in PANC1 cell line stimulated with TGFβ1. Our results support downregulation of E-cadherin protein. We show 5- and 28-fold increase in SNAI1 and SNAI2 expression levels and 25- and 15-fold decrease in CDH1 and KRT8 expression levels, respectively, which confirms the EMT-program fulfillment. We demonstrate downregulation of expression of pancreatic master genes SOX9, FOXA2, and GATA4 (2-, 5-, and 4-fold, respectively) and absence of significant changes in HES1, NR5A2, and GATA6 expression levels in the cells stimulated with TGFβ1. Our results indicate the absence of induction of expression of PTF1A, PDX1, HNF1b, NEUROG3, RPBJL, NKX6.1, and ONECUT1 genes, which are inactive in PANC1 cell line after the EMT stimulated by TGFβ1.

  2. Kondratyeva L.G., Vinogradova T.V., Chernov I.P., Sverdlov E.D. (2015). [Master Transcription Regulators Specifying Cell-Lineage Fates in Development As Possible Therapeutic Targets in Oncology]. Genetika 51 (11), 1221–33 [+]

    The transformation of normal precursors into cancer cells is an intricately regulated, multistep process. The master regulatory genes that play a crucial role in the process of organism development may also play a key role in carcinogenesis. From such a point of view, cancer is not simply a genetic disease that is due to a progressive accumulation of mutation--it is also a disorder of the developmental system of the tissue in which cancer emerges. Master regulators and their genes disturb stem cell differentiation upon mutation and thus may serve as targets for cancer therapy, in addition to the classic oncogenes and suppressors of tumor formation. This review is an attempt to give a modern concept of master genes and their functions in adult stem cells of the organism and in carcinogenesis, with pancreatic cancer as an example.

  3. Vinogradova T.V., Chernov I.P., Monastyrskaya G.S., Kondratyeva L.G., Sverdlov E.D. (2009). Cancer Stem Cells: Plasticity Works against Therapy. Acta Naturae 7 (4), 46–55 [+]

    Great successes in identification and deciphering of mechanisms of the adult stem cells regulation have given rise to the idea that stem cells can also function in tumors as central elements of their development, starting from the initial stage and continuing until metastasis. Such cells were called cancer stem cells (CSCs). Over the course of intense discussion, the CSCs hypothesis gradually began to be perceived as an obvious fact. Recently, the existence of CSCs has been indeed confirmed in a number of works. However, when are CSCs universal prerequisites of tumors and to what extent their role is essential for tumor evolution remains an issue far from resolved. Likewise, the problem of potential use of CSCs as therapeutic targets remains unsolved. The present review attempts to analyze the issue of cancer stem cells and the potential of targeting them in tumor therapy.