Образование

Избранные публикации

1. Pogorelyy M., PuelmaTouzer M., Minervina A.A., Sycheva A.L., Chudakov D.M., Mamedov I.Z., Mora T., Walczak A.M., Lebedev Y.B. (2017). High throughput sequencing of identical twins TCR repertoires after yellow fever vaccination. Systems Biology of Adaptive Immunity – Congressi Stefano Franscini, Monte Verita, Ascona, Switzerland – 14-17 May 2017. Abstract book , 60 ID:1888
2. Nazarov V.I., Minervina A.A., Komkov A.Y., Pogorelyy M.V., Maschan M.A., Olshanskaya Y.V., Zvyagin I.V., Chudakov D.M., Lebedev Y.B., Mamedov I.Z. (2016). Reliability of immune receptor rearrangements as genetic markers for minimal residual disease monitoring. Bone marrow transplantation , ID:1532
3. Minervina A.A., Komkov A.Y., Mamedov I.Z., Lebedev Y.B. (2016). Advanced lymphoblastic clones detection in T-cell leukemia. Dokl. Biochem. Biophys. 467 (1), 85–8 [+]

   T cell acute lymphoblastic leukemia (T-ALL) is an aggressive malignant neoplasm of the lymphocyte precursors that suffered malignant transformation arresting the lymphoid cell differentiation. Clinical studies revealed monoor, more rarely, oligoclonal nature of the disease. A precise identification of malignant clone markers is both the crucial stage of early diagnostics and the essential prognostic factor for therapeutic treatment. Here we present an improved system for unbiased detection of lymphoblastic clones in bone marrow aspirates of T-ALL patients. The system based on multiplex PCR of rearranged T-cell receptor locus (TRB) and straightforward sequencing of the resulted PCR fragments. Testing of the system on genomic DNA from Jurkat cell line and four clinical bone marrow aspirates revealed a set of unique TRB rearrangements that precisely characterize each of tested samples. Therefore, the outcome of the system produces highly informative molecular genetic markers for further monitoring of minimal residual disease in T-ALL patients.

   ID:1874
4. Kurnosov A.A., Ustyugova S.V., Nazarov V.I., Minervina A.A., Komkov A.Y., Shugay M., Pogorelyy M.V., Khodosevich K.V., Mamedov I.Z., Lebedev Y.B. (2014). The evidence for increased L1 activity in the site of human adult brain neurogenesis. PLoS ONE 10 (2), e0117854 [+]

   Retroelement activity is a common source of polymorphisms in human genome. The mechanism whereby retroelements contribute to the intraindividual genetic heterogeneity by inserting into the DNA of somatic cells is gaining increasing attention. Brain tissues are suspected to accumulate genetic heterogeneity as a result of the retroelements somatic activity. This study aims to expand our understanding of the role retroelements play in generating somatic mosaicism of neural tissues. Whole-genome Alu and L1 profiling of genomic DNA extracted from the cerebellum, frontal cortex, subventricular zone, dentate gyrus, and the myocardium revealed hundreds of somatic insertions in each of the analyzed tissues. Interestingly, the highest concentration of such insertions was detected in the dentate gyrus-the hotspot of adult neurogenesis. Insertions of retroelements and their activity could produce genetically diverse neuronal subsets, which can be involved in hippocampal-dependent learning and memory.

   ID:1274