Шипунова Виктория Олеговна


Период обученияСтрана, городУчебное заведениеДополнительная информация
2007–2013 Москва, Россия Московский Физико-Технический Институт (ГУ) Диплом магистра с отличием по направлению "Биотехнология".

Научные интересы

Наночастицы, адресная доставка лекарственных препаратов, биосенсоры, умные материалы

Премии и заслуги

1) Октябрь 2011 Премия 1 степени IV Международного конкурса научных работ молодых ученых в области нанотехнологий. RusNanoTech-2011, Москва, Россия.

2) 2012-2013 Повышенная государственная академическая стипендия за достижения в научно-исследовательской работе.

3) Февраль 2013 Премия 3 степени Конкурса молодых ученых в рамках XXV Зимней молодёжной научной школы “Перспективные направление физико-химической биологии и биотехнологии”, Москва, Россия.

4) Июль 2013 Победитель Всероссийского молодежного конкурса научных работ по современным проблемам фундаментальных и прикладных наук, Международная научная школа для молодёжи и преподавателей "Прикладные математика и физика: от фундаментальных исследований к инновациям", МФТИ(ГУ), г. Долгопрудный.

5) Июль 2013 Победитель конкурса магистерских диссертаций по направлению "Прикладные математика и физика", Международная научная школа для молодёжи и преподавателей "Прикладные математика и физика: от фундаментальных исследований к инновациям", МФТИ(ГУ), г. Долгопрудный, Россия.

6) Ноябрь 2013 Победитель конкурса Фонда содействия малых форм предприятий в научно-технической сфере У.М.Н.И.К., г. Долгопрудный, Россия.

7) Июнь 2014 Премия 2 степени на конкурсе стендовых докладов на 10th International Conference on the Scientific and Clinical Applications of Magnetic Carriers, Дрезден, Германия.

8) Февраль 2015 Стипендия Президента Российской Федерации для аспирантов и молодых ученых.

Членство в научных обществах


Избранные публикации

  1. Shipunova V.O., Nikitin M.P., Nikitin P.I., Deyev S.M. (2016). MPQ-cytometry: a magnetism-based method for quantification of nanoparticle-cell interactions. Nanoscale , [+]

    Precise quantification of interactions between nanoparticles and living cells is among the imperative tasks for research in nanobiotechnology, nanotoxicology and biomedicine. To meet the challenge, a rapid method called MPQ-cytometry is developed, which measures the integral non-linear response produced by magnetically labeled nanoparticles in a cell sample with an original magnetic particle quantification (MPQ) technique. MPQ-cytometry provides a sensitivity limit 0.33 ng of nanoparticles and is devoid of a background signal present in many label-based assays. Each measurement takes only a few seconds, and no complicated sample preparation or data processing is required. The capabilities of the method have been demonstrated by quantification of interactions of iron oxide nanoparticles with eukaryotic cells. The total amount of targeted nanoparticles that specifically recognized the HER2/neu oncomarker on the human cancer cell surface was successfully measured, the specificity of interaction permitting the detection of HER2/neu positive cells in a cell mixture. Moreover, it has been shown that MPQ-cytometry analysis of a HER2/neu-specific iron oxide nanoparticle interaction with six cell lines of different tissue origins quantitatively reflects the HER2/neu status of the cells. High correlation of MPQ-cytometry data with those obtained by three other commonly used in molecular and cell biology methods supports consideration of this method as a prospective alternative for both quantifying cell-bound nanoparticles and estimating the expression level of cell surface antigens. The proposed method does not require expensive sophisticated equipment or highly skilled personnel and it can be easily applied for rapid diagnostics, especially under field conditions.

  2. Shipunova V.O., Nikitin M.P., Zelepukin I.V., Nikitin P.I., Deyev S.M., Petrov R.V. (2015). A comprehensive study of interactions between lectins and glycoproteins for the development of effective theranostic nanoagents. Dokl. Biochem. Biophys. 464 (1), 315–8 [+]

    A comprehensive study of the interactions between lectins and glycoproteins possessing different glycosylation profiles in the composition of nanoparticles was carried out in order to find specifically interacting protein pairs for the creation of novel classes of multifunctional nanoagets that based on protein-assisted selfassembly. We obtained information about specific interactions of certain lectins with selected glycoproteins as well as about the ability of certain monosaccharides to competitively inhibit binding of glycoproteins with lectins. These protein-mediated interactions may be involved in the formulation of self-assembled nanoparticles for therapy and diagnostics of various diseases.

  3. Nikitin M.P., Shipunova V.O., Deyev S.M., Nikitin P.I. (2014). Biocomputing based on particle disassembly. Nat Nanotechnol , [+]

    Nanoparticles with biocomputing capabilities could potentially be used to create sophisticated robotic devices with a variety of biomedical applications, including intelligent sensors and theranostic agents. DNA/RNA-based computing techniques have already been developed that can offer a complete set of Boolean logic functions and have been used, for example, to analyse cells and deliver molecular payloads. However, the computing potential of particle-based systems remains relatively unexplored. Here, we show that almost any type of nanoparticle or microparticle can be transformed into autonomous biocomputing structures that are capable of implementing a functionally complete set of Boolean logic gates (YES, NOT, AND and OR) and binding to a target as result of a computation. The logic-gating functionality is incorporated into self-assembled particle/biomolecule interfaces (demonstrated here with proteins) and the logic gating is achieved through input-induced disassembly of the structures. To illustrate the capabilities of the approach, we show that the structures can be used for logic-gated cell targeting and advanced immunoassays.

  4. Orlov A.V., Burenin A.G., Shipunova V.O., Lizunova A.A., Gorshkov B.G., Nikitin P.I. (2014). Development of immunoassays using interferometric real-time registration of their kinetics. Acta Naturae 6 (1), 85–95 [+]

    A method for effective development of solid-phase immunoassays on a glass surface and for optimization of related protocols by highly sensitive quantitative monitoring of each assay step has been proposed and experimentally implemented. The method is based on the spectral correlation interferometry (SCI) that allows real-time measuring of the thickness of a biomolecular layer bound to the recognition molecular receptors on the sensor chip surface. The method is realized with compact 3-channel SCI-biosensors that employ as the sensor chips standard cover glass slips without deposition of any additional films. Different schemes for antibody immobilization on a glass surface have been experimentally compared and optimized toward a higher sorption capacity of the sensor chips. Comparative characterization of the kinetics of each immunoassay stage has been implemented with the optimized protocols: i) covalent immobilization of antibody on an epoxylated surface and ii) biotinylated antibody sorption on a biotinylated surface via a high-affinity biotin-streptavidin bond. We have shown that magnetic nanoparticles employed as labels with model detection of cardiac troponin I further amplify the SCI signal, resulting in 100-fold improvement of the detection limit. The developed protocols can also be used with the alternative immunoassay platforms, including the label methods based on registration of only the final assay result, which is the quantity of bound labels.

  5. Shipunova V.O., Nikitin M.P., Lizunova A.A., Ermakova M.A., Deyev S.M., Petrov R.V. (2013). Polyethyleneimine-coated magnetic nanoparticles for cell labeling and modification. Dokl. Biochem. Biophys. 452 (1), 245–7 ID:1266
  6. Zelepukin I.V., Shipunova V.O., Mirkasymov A.B., Nikitin P.I., Nikitin M.P., Deyev S.M. (2009). Synthesis and Characterization of Hybrid Core-Shell Fe3O4/SiO2 Nanoparticles for Biomedical Applications. Acta Naturae 9 (4), 58–65 [+]

    The creation of markers that provide both visual and quantitative information is of considerable importance for the mapping of tissue macrophages and other cells. We synthesized magnetic and magneto-fluorescent nanomarkers for the labeling of cells which can be detected with high sensitivity by the magnetic particle quantification (MPQ) technique. For stabilization under physiological conditions, the markers were coated with a dense silica shell. In this case, the size and zeta-potential of nanoparticles were controlled by a modified Stober reaction. Also, we developed a novel facile two-step synthesis of carboxylic acid-functionalized magnetic SiOnanoparticles, with a carboxyl polymer shell forming on the nanoparticles before the initiation of the Stober reaction. We extensively characterized the nanomarkers by transmission electron microscopy, electron microdiffraction, and dynamic and electrophoretic light scattering. We also studied the nanoparticle cellular uptake by various eukaryotic cell lines.