Шрамова Елена Ивановна

Кандидат биологических наук

Младший научный сотрудник (Лаборатория молекулярной иммунологии)

Тел.: +7 (916) 950-35-49

Эл. почта: fei@psha.org.ru

Личная информация

Начала работу в ИБХ в 2000 г. студенткой 3-курса МФТИ в составе группы Трансгеноза. Кандидатскую диссертацию защищала под руководством Зацепиной Ольги Владимировны в ИБР РАН в 2008. С 2008 по 2011 гг и с 2012 по 2015 гг была в декретах. С 2011 года после расформирования группы Трансгеноза и по настоящее время работаю в Лаборатории молекулярной иммунологии.


Период обученияСтрана, городУчебное заведениеДополнительная информация
1986–1993 Россия, Москва Средняя школа №689
1993–1997 Россия, Москва Физико-математическая школа-лаборатория №444
1997–2017 Россия, Долгопрудный МФТИ(ГУ) факультет молекулярной и биологической физики диплом с отличием
2017 Россия, Долгопрудный МФТИ(ГУ) факультет молекулярной и биологической физики, аспирантура

Научные интересы

Фотодинамическая терапия, цитотоксические белки, гибридные клетки, клеточная дифференцировка.

Избранные публикации

  1. Shramova E.I., Proshkina G.M., Deyev S.M., Petrov R.V. (2017). Flavoprotein miniSOG BRET-induced cytotoxicity depends on its intracellular localization. Dokl. Biochem. Biophys. 474 (1), 228–230 [+]

    It is proposed to use the bioluminescent resonance energy transfer to solve the problem of creating the internal light sources in photodynamic therapy of cancer. Energy donor in the developed system is the oxidized form of the luciferase NanoLuc substrate furimamide, and acceptor is the phototoxic fluorescent protein miniSOG. It is shown that, in the proposed system, the photoinduced cytotoxicity of flavoprotein miniSOG in vitro depends on the intracellular localization, and the cytotoxic effect is 48% for the cytoplasmic localization of the fusion protein, 65% for the mitochondrial localization, and 69% for the membrane localization. The obtained data indicate that, for maximization of the photodynamic effect in vivo, it is appropriate to use the NanoLuc-miniSOG fusion protein in the membrane localization.

  2. Shamova O.V., Orlov D.S., Balandin S.V., Shramova E.I., Tsvetkova E.V., Panteleev P.V., Leonova Y.F., Tagaev A.A., Kokryakov V.N., Ovchinnikova T.V. (2014). Acipensins - Novel Antimicrobial Peptides from Leukocytes of the Russian Sturgeon Acipenser gueldenstaedtii. Acta Naturae 6 (4), 99–109 [+]

    Antimicrobial peptides (AMPs) play an important role in the innate defense mechanisms in humans and animals. We have isolated and studied a set of antimicrobial peptides from leukocytes of the Russian sturgeon Acipenser gueldenstaedtii belonging to a subclass of chondrosteans, an ancient group of bony fish. Structural analysis of the isolated peptides, designated as acipensins (Ac), revealed in leukocytes of the Russian sturgeon six novel peptides with molecular masses of 5336.2 Da, 3803.0 Da, 5173.0 Da, 4777.5 Da, 5449.4 Da, and 2740.2 Da, designated as Ac1-Ac6, respectively. Complete primary structures of all the isolated peptides were determined, and the biological activities of three major components - Ac1, Ac2, and Ac6 - were examined. The peptides Ac1, Ac2, Ac3, Ac4, and Ac5 were found to be the N-terminal acetylated fragments 1-0, 1-5, 1-9, 1-4, and 1-1 of the histone H2A, respectively, while Ac6 was shown to be the 62-5 fragment of the histone H2A. The peptides Ac1 and Ac2 displayed potent antimicrobial activity towards Gram-negative and Gram-positive bacteria (Escherichia coli ML35p, Listeria monocytogenes EGD, MRSA ATCC 33591) and the fungus Candida albicans 820, while Ac6 proved effective only against Gram-negative bacteria. The efficacy of Ac 1 and Ac2 towards the fungus and MRSA was reduced upon an increase in the ionic strength of the solution. Ac1, Ac2, and Ac6, at concentrations close to their minimum inhibitory concentrations, enhanced the permeability of the E.coli ML35p outer membrane to the chromogenic marker, but they did not affect appreciably the permeability of the bacterial inner membrane in comparison with a potent pore-forming peptide, protegrin 1. Ac1, Ac2, and Ac6 revealed no hemolytic activity against human erythrocytes at concentrations of 1 to 40 μM and had no cytotoxic effect (1 to 20 μM) on K-562 and U-937 cells in vitro. Our findings suggest that histone-derived peptides serve as important anti-infective host defense molecules.

  3. Bozhenko V.K., Shramova E.I., Shishkin A.M., Ivanov A.V., Khokhlova E.V., Lebedin Y.S., Shkoporov A.N. (2013). Characteristics of new monomolecular chimeric T-cell receptors to carcinoembryonic antigen. Bull. Exp. Biol. Med. 156 (1), 165–71 [+]

    We described two original genetic constructs encoding chimeric monomolecular T-cell receptors, where the effector T-cell receptor fragment was linked with the antigen-recognizing part consisting of two variable fragments of two different antibodies to carcinoembryonic antigen. Following transfection, these receptors were expressed on the cell surface and bound carcinoembryonic antigen. Human peripheral blood lymphocytes transfected with the above constructs demonstrated high cytotoxic activity against HCT116 cells expressing carcinoembryonic antigen.

  4. Shramova E.I., Proshkina G.M., Chumakov S.P., Khodarovich Y.M., Deyev S.M. (2009). Flavoprotein miniSOG Cytotoxisity Can Be Induced By Bioluminescence Resonance Energy Transfer. Acta Naturae 8 (4), 118–123 [+]

    In this study, we investigated the possibility of phototoxic flavoprotein miniSOG (photosensitizer) excitation in cancer cells by bioluminescence occurring when luciferase NanoLuc oxidizes its substrate, furimazine. We have shown that the phototoxic flavoprotein miniSOG expressed in eukaryotic cells in fusion with NanoLuc luciferase is activated in the presence of its substrate, furimazine. Upon such condition, miniSOG possesses photoinduced cytotoxicity and causes a 48% cell death level in a stably transfected cell line.

  5. Filyasova E.I., Zatsepina O.V., Larionov O.A., Khodarovich Y.M. (2009). Obtaining hybrid mammalian cells containing diploid chromosome number. Dokl. Biol. Sci. 411, 520–3 ID:1857
  6. Finkina E.I., Shramova E.I., Tagaev A.A., Ovchinnikova T.V. (2008). A novel defensin from the lentil Lens culinaris seeds. Biochem. Biophys. Res. Commun. 371 (4), 860–5 [+]

    A novel 47-residue plant defensin was purified from germinated seeds of the lentil Lens culinaris by ammonium sulfate precipitation, gel filtration, chromatography, and RP-HPLC. The molecular mass (5440.41Da) and complete amino acid sequence (KTCENLSDSFKGPCIPDGNCNKHCKEKEHLLSGRCRDDFRCWCTRNC) of defensin, termed Lc-def, were determined. Lc-def has eight cysteines forming four disulfide bonds. The total RNA was isolated from lentil germinated seeds, RT-PCR and subsequent cloning were performed, and cDNA was sequenced. A 74-residue predefensin contains a putative signal peptide (27 amino acid) and a mature protein. Lc-def shows high sequence homology with legumes defensins, exhibits an activity against Aspergillus niger, but does not inhibit proteolytic enzymes.

  7. Kulinich T.M., Kharchenko V.P., Filyasova E.I., Shishkin A.M., Bozhenko V.K. (2008). Cytostatic and cytotoxic properties of chimeric peptides containing cyclin-inhibiting fragments. Bull. Exp. Biol. Med. 145 (1), 37–40 [+]

    Properties of chimeric peptides containing cell-penetrating sequences and pl6INK4a and E2F fragments were studied in vitro on immurtal cultures of human cells. Both sequences exhibit cytostatic activity. The peptide containing fragment p16INK4a inhibited proliferation during the G0/G1 stage of the cell cycle, while sequence E2F suppressed proliferation in S phase. Both sequences exhibit cytotoxic properties and can induce apoptosis.

  8. Shramova E.I., Khodarovich Iu.M., Larionov O.A., Zatsepina O.V. (2008). [The status of nucleolus organizing regions in hybrids of pluripotent and somatic mouse cells cultured under different conditions]. Tsitologiia 50 (4), 302–8 [+]

    In the present work we examined the status of nucleolus organizing regions of mitotic chromosomes (NOR) in hybrid cells obtained by fusion of the mouse teratocarcinoma cells PCC4aza1 and adult mouse spleenocytes upon cultivation of hybrid cells under different conditions. We have shown that extended cultivation of hybrid cells in medium supplemented with HAT (hypoxanthine, aminopterin, thymidine) promotes the maintenance of NO-chromosomes, whereas under nonselective conditions elimination of NO-chromosome occurs. In nonselective medium the number of active, i. e. Ag-positive, NORs has been augmented comparatively to that observed under selective conditions. This observation directly indicates that reprogramming of the parental cell genomes in hybrid cells includes changes in the status of chromosomal NORs. The number of active NORs depends on conditions of hybrid cells culturing and may be changed by either of the two major ways--by elimination of NO-chromosomes (under nonselective conditions) or by inactivation of some NORs, when the general number of NO-chromosomes remains unaltered (under selective conditions).