Шрамова Елена Ивановна

Кандидат биологических наук

Младший научный сотрудник (Лаборатория молекулярной иммунологии)

Тел.: +7 (495) 330-64-65

Эл. почта: fei@psha.org.ru

Избранные публикации

  1. Finkina E.I., Shramova E.I., Tagaev A.A., Ovchinnikova T.V. (2008). A novel defensin from the lentil Lens culinaris seeds. Biochem. Biophys. Res. Commun. 371 (4), 860–5 [+]

    A novel 47-residue plant defensin was purified from germinated seeds of the lentil Lens culinaris by ammonium sulfate precipitation, gel filtration, chromatography, and RP-HPLC. The molecular mass (5440.41Da) and complete amino acid sequence (KTCENLSDSFKGPCIPDGNCNKHCKEKEHLLSGRCRDDFRCWCTRNC) of defensin, termed Lc-def, were determined. Lc-def has eight cysteines forming four disulfide bonds. The total RNA was isolated from lentil germinated seeds, RT-PCR and subsequent cloning were performed, and cDNA was sequenced. A 74-residue predefensin contains a putative signal peptide (27 amino acid) and a mature protein. Lc-def shows high sequence homology with legumes defensins, exhibits an activity against Aspergillus niger, but does not inhibit proteolytic enzymes.