Laboratory of molecular technologies
In our research we apply synthetic biology principles to study redox signaling, to develop molecular tools for in vivo imaging, metabolic engineering and optogenetics. In addition, we study molecular mechanisms of ischemic pathology, signaling in cancer cells, an interplay between calcium and reactive oxygen species and other relevant topics. The central principle of our research is trans-species and even trans-kingdom transfer of molecular blocks in order to obtain engineered living system with new properties. Our achievements in the field of redox biology and fluorescent microscopy include development of sensors for important redox active compounds within living cells, novel tools for optogenetics, new methods of super resolution microscopy etc.
- novel tools and instrumentation for thermogenetics (Fedotov et al, 2015; Safronov et al, 2015; Ermakova et al, 2017);
- a first application of a biosensor in subdiffraction microscopy (Mishina at al, 2015);
- SypHer2, a genetically encoded pH sensor allowing synaptic pH imaging (Matlashov et al, 2015);
- development of the red fluorescent genetically encoded H2O2 sensor (Ermakova et al, 2014);
- development of genetically encoded H2O2 generation/detection system based on fusion of D-amino acid oxidase and HyPer (Matlashov et al, 2014);
- development of the genetically encoded fluorescent sensor for intracellular NAD+/NADH ratio (Bilan et al, 2014);
- development of HyPer-3, the high dynamic range and fast-responding H2O2 probe for ratiometric and lifetime imaging (Bilan et al, 2013);
- first records of H2O2 production by phagocytosing macrophages at the level of single cell (Mishina et al, 2013);
- discovery of polarized H2O2 production in the immunological synapse (Mishina et al, 2012)
- discovery of H2O2 microdomains in the cells stimulated with growth factors, the first direct demonstration of local H2O2 signaling (Mishina et al., 2011);
- development of HyPer-2, improved version of HyPer with expanded dynamic range (Markvicheva et al., 2011);
- development of HyPer, sensor for H2O2 (Belousov et al, 2006).
|Vsevolod Belousov, D.Sc||Head of firstname.lastname@example.org, |
|Nataliya Mishina, Ph.D.||s. r. email@example.com, |
|Ilya Kelmanson, Ph.D.||r. firstname.lastname@example.org|
|Kseniya Markvicheva, Ph.D.||r. f.|
|Yulia Bogdanova||j. r. email@example.com|
|Ekaterina Potehina, Ph.D.||j. r. firstname.lastname@example.org|
|Ksenia Shishova||j. r. f.|
|Elena Fetisovaemail@example.com, |
|Aram Revazyan||res. firstname.lastname@example.org|
|Dina Bassemail@example.com, |
|Alexander Ivanenkofirstname.lastname@example.org, |
|Dmitry Bilan, Ph.D.||s. r. email@example.com|
|Yuliya Ermakova, Ph.D.||s. r. firstname.lastname@example.org|
|Elena Kudryavtseva, Ph.D.||r. email@example.com|
|Marina Roshina||r. f.|
|Mikhail Matlashov||PhD firstname.lastname@example.org|
|Arina Shokhina||PhD email@example.com|
Chemogenetic approaches for visualization of cellular antioxidant activity
Hydrogen peroxide is formed under the action of many enzymes and performs signaling functions locally. Its diffusion in the cell is limited by the action of antioxidant systems. This contributes to the occurrence of locally elevated concentrations of ROS, sufficient for the implementation of regulatory functions, while not causing damage. At the moment, the contribution of each antioxidant system to this process was not completely clear. Visualization of the action of antioxidant systems is rather complicated: enzymes that generate hydrogen peroxide have their localization pattern, as well as the main members of antioxidant systems, both patterns are poorly understood.
To solve this problem, the principles of chemogenetics and synthetic biology were used. The enzyme D-amino acid oxidase (DAAO), which performs the oxidative deamination of D-amino acids with the formation of hydrogen peroxide as a by-product, has already been successfully used for the controlled production of H2O2 in various cells types, as well as in vivo. Using a special peptide signal, this enzyme was localized in the cell nucleus, while the HyPer3 biosensor was localized in the mitochondrial matrix. Both compartments maintained their positions in the cell for a time sufficient to visualize the gradient of hydrogen peroxide. This gradient was formed after the activation of DAAO by the addition of a D-amino acid as a result of the action of antioxidant systems that prevented diffusion of the resulting H2O2. Further, using specific inhibitors, it was shown that the thioredoxin pathway plays a key role in limiting the diffusion of hydrogen peroxide in the cytoplasm of HeLa Kyoto cells. Both thioredoxin reductases in the cytoplasm and mitochondria are equally important in this process. The system allows to study the contributions of antioxidant enzymes to the formation of local redox balance in various types of cells, as well as to carry out simple screenings of substances that act on antioxidant systems of cells.
- (2019). Which antioxidant system shapes intracellular H2O2 gradients? Antioxid Redox Signal 31 (9), 664–670
Genetically encoded indicator Grx1-roCherry based on red fluorescent protein for detection of glutathione redox status
In collaboration with Group of metabolic bases of pathology
We developed a genetically encoded biosensor for registration the redox state of the glutathione pool (2GSH/GSSG ratio) based on the red fluorescent protein mCherry. The structure of the fluorescent protein contains a pair of redox-active cysteines that are involved in the thiol-disulfide exchange reactions of intracellular systems. The fluorescent signal of the biosensor reflects the redox state of glutathione in the studied system due to the spectral difference between the oxidized and reduced forms of the protein. Human glutaredoxin-1 was added to the structure to improve the kinetic properties of the biosensor. Grx1-roCherry biosensor is a reliable tool for monitoring changes in the 2GSH/GSSG ratio in real time in various biological systems, including a combination with spectrally different versions in a multiparameter imaging mode.
- (2018). Red fluorescent redox-sensitive biosensor Grx1-roCherry. Redox Biol 21, 101071
Genetically encoded fluorescent pH probe for precise monitoring of cellular biochemistry
This “molecular pH-meter” allows the quantitative measurement of pH in living systems of various complexity. Using genetic engineering methods, SypHer3s can be delivered into a living cell and, due to its high brightness, can be used in high-resolution microscopy: for example, for accurate observations of fluctuations in acidity in cells or even whole organisms — the article describes a first measurement of pH in various tissues of the zebrafish embryo.
In addition, SypHer3s helped to demonstrate the functional heterogeneity of mitochondria in different compartments of neurons. In the body of a neuron, mitochondria are inactive, while in synapses they begin to actively pump out protons from the matrix creating an electrochemical gradient necessary for the synthesis of ATP. It is convenient to monitor these processes using the pH probe directed to the mitochondria. According to Vsevolod Belousov, “it seems that different parts of the neuron get the energy in different ways: the body uses glycolysis, and synapses — oxidative phosphorylation”.
- (2018). SypHer3s: A genetically encoded fluorescent ratiometric probe with enhanced brightness and an improved dynamic range. Chem Commun (Camb) 54 (23), 2898–2901
Chemogenetic model of cardiac failure
Researchers from Molecular technologies laboratory (IBCh) in collaboration with Harvard medical school scientists developed a novel model of cardiac dysfunction caused by oxidative stress. This model is based on chemogenetics principles — enzymatic production of reactive oxygen species (ROS), stimulated by an external chemical substrate, and visualised by transgenic ROS sensor HyPer. The study is supported by Russian science foundation and published in Nature Communications.
- (2018). Chemogenetic generation of hydrogen peroxide in the heart induces severe cardiac dysfunction. Nat Commun 9 (1), 4044