Laboratory of proteomics
Alternative splicing shapes transcriptome but not proteome diversity in Physcomitrella patens (2017-12-05)
Alternative splicing (AS) can significantly impact the transcriptome and proteome of a eukaryotic cell. We analyzed the extent to which AS contributes to proteome diversity using moss Physcomitrella patens as a model object. We could distinguish a few examples that unambiguously indicated the presence of two or more splice isoforms from the same locus at the proteomic level. Our results indicate that alternative isoforms have a small effect on proteome diversity. We also revealed that mRNAs and pre-mRNAs have thousands of complementary binding sites for long non-coding RNAs (lncRNAs) that may lead to potential interactions in transcriptome. This finding points to an additional level of gene expression and AS regulation by non-coding transcripts in Physcomitrella patens.
- (2017). Alternative splicing shapes transcriptome but not proteome diversity in Physcomitrella patens. Sci Rep 7 (1), 2698