Уткин Юрий Николаевич

Научные интересы

Научные интересы Ю.Н.Уткина лежат в области исследования ядов животных, включая поиск в ядах, выделение и изучение биологической активности белков с новыми фармакологическими свойствами. Основное направление исследований – полипептидные соединения, взаимодействующие с Cys-петельными рецепторами.

Премии и заслуги

 

Ю.Н.Уткин награжден Почетной грамотой Российской  академии наук «За многолетнюю и плодотворную работу в Российской  академии наук и в связи с 275-летием Академии» и медалью «К 850-летию Москвы», являлся лауреатом конкурса на присуждение Государственных научных стипендий для ученых (1997-2002 гг.).

Членство в научных обществах

Член

международного общества токсинологии, европейского нейрохимического общества, американского общества биохимии и молекулярной биологии.

Избранные публикации

  1. Shelukhina IV, Zhmak MN, Lobanov AV, Ivanov IA, Garifulina AI, Kravchenko IN, Rasskazova EA, Salmova MA, Tukhovskaya EA, Rykov VA, Slashcheva GA, Egorova NS, Muzyka IS, Tsetlin VI, Utkin YN (2018). Azemiopsin, a selective peptide antagonist of muscle nicotinic acetylcholine receptor: Preclinical evaluation as a local muscle relaxant. Toxins (Basel) 10 (1),
  2. Dubovskii PV, Dubinnyi MA, Volynsky PE, Pustovalova YE, Konshina AG, Utkin YN, Arseniev AS, Efremov RG (2017). Impact of membrane partitioning on the spatial structure of an S-type cobra cytotoxin. J Biomol Struct Dyn  (0), 1–16
  3. Tran TV, Hoang AN, Nguyen TTT, van Phung T, Nguyen KC, Osipov AV, Ivanov IA, Tsetlin VI, Utkin YN (2017). Anticoagulant activity of low-molecular weight compounds from heterometrus laoticus scorpion venom. Toxins (Basel) 9 (11),
  4. Vulfius CA, Kasheverov IE, Kryukova EV, Spirova EN, Shelukhina IV, Starkov VG, Andreeva TV, Faure G, Zouridakis M, Tsetlin VI, Utkin YN (2017). Pancreatic and snake venom presynaptically active phospholipases A2inhibit nicotinic acetylcholine receptors. PLoS One 12 (10), e0186206
  5. Osipov AV, Terpinskaya TI, Kuznetsova TE, Ryzhkovskaya EL, Lukashevich VS, Rudnichenko JA, Ulashchyk VS, Starkov VG, Utkin YN (2017). Cobra venom factor and ketoprofen abolish the antitumor effect of nerve growth factor from cobra venom. Toxins (Basel) 9 (9),
  6. Lyukmanova EN, Shulepko MA, Shenkarev ZO, Kasheverov IE, Chugunov AO, Kulbatskii DS, Myshkin MY, Utkin YN, Efremov RG, Tsetlin VI, Arseniev AS, Kirpichnikov MP, Dolgikh DA (2016). Central loop of non-conventional toxin WTX from Naja kaouthia is important for interaction with nicotinic acetylcholine receptors. Toxicon 119 (0), 274–279
  7. Kovalchuk SI, Ziganshin RH, Starkov VG, Tsetlin VI, Utkin YN (2016). Quantitative proteomic analysis of venoms from Russian vipers of Pelias group: Phospholipases A2are the main venom components. Toxins (Basel) 8 (4), 105
  8. Utkin YN, Krivoshein AV (2016). Snake venoms and envenomation: Modern trends and future prospects.  (0), 1–303
  9. Utkin YN, Krivoshein AV (2016). Preface.  (0), xi–x
  10. Lyukmanova EN, Shenkarev ZO, Shulepko MA, Paramonov AS, Chugunov AO, Janickova H, Dolejsi E, Dolezal V, Utkin YN, Tsetlin VI, Arseniev AS, Efremov RG, Dolgikh DA, Kirpichnikov MP (2015). Structural insight into specificity of interactions between nonconventional three-finger weak toxin from Naja kaouthia (WTX) and muscarinic acetylcholine receptors. J Biol Chem 290 (39), 23616–23630
  11. Kudryavtsev DS, Shelukhina IV, Son LV, Ojomoko LO, Kryukova EV, Lyukmanova EN, Zhmak MN, Dolgikh DA, Ivanov IA, Kasheverov IE, Starkov VG, Ramerstorfer J, Sieghart W, Tsetlin VI, Utkin YN (2015). Neurotoxins from snake venoms and α-Conotoxin ImI inhibit functionally active Ionotropic γ-aminobutyric acid (GABA) receptors. J Biol Chem 290 (37), 22747–22758
  12. Kudryavtsev D, Shelukhina I, Vulfius C, Makarieva T, Stonik V, Zhmak M, Ivanov I, Kashevero I, Utkin Y, Tsetlin V (2015). Natural compounds interacting with nicotinic acetylcholine receptors: From low-molecular weight ones to peptides and proteins. Toxins (Basel) 7 (5), 1683–1701
  13. Dubovskii PV, Utkin YN (2015). Antiproliferative activity of cobra venom cytotoxins. Curr Top Med Chem 15 (7), 638–648
  14. Ghazaryan NA, Ghulikyan L, Kishmiryan A, Andreeva TV, Utkin YN, Tsetlin VI, Lomonte B, Ayvazyan NM (2015). Phospholipases a2from Viperidae snakes: Differences in membranotropic activity between enzymatically active toxin and its inactive isoforms. BIOCHIM BIOPHYS ACTA 1848 (2), 463–468
  15. Vulfius CA, Kasheverov IE, Starkov VG, Osipov AV, Andreeva TV, Filkin SY, Gorbacheva EV, Astashev ME, Tsetlin VI, Utkin YN (2014). Inhibition of Nicotinic Acetylcholine Receptors, a Novel Facet in the Pleiotropic Activities of Snake Venom Phospholipases A2. PLoS One 9 (12), e115428
  16. Osipov AV, Terpinskaya TI, Kryukova EV, Ulaschik VS, Paulovets LV, Petrova EA, Blagun EV, Starkov VG, Utkin YN (2014). Nerve growth factor from cobra venom inhibits the growth of Ehrlich tumor in mice. Toxins (Basel) 6 (3), 784–795
  17. Utkin YN, Weise C, Kasheverov IE, Andreeva TV, Kryukova EV, Zhmak MN, Starkov VG, Hoang NA, Bertrand D, Ramerstorfer J, Sieghart W, Thompson AJ, Lummis SCR, Tsetlin VI (2012). Azemiopsin from Azemiops feae viper venom, a novel polypeptide ligand of nicotinic acetylcholine receptor. J Biol Chem 287 (32), 27079–27086
  18. Osipov AV, Rucktooa P, Kasheverov IE, Filkin SY, Starkov VG, Andreeva TV, Sixma TK, Bertrand D, Utkin YN, Tsetlin VI (2012). Dimeric α-Cobratoxin X-ray structure: Localization of intermolecular disulfides and possible mode of binding to nicotinic acetylcholine receptors. J Biol Chem 287 (9), 6725–6734
  19. Alama A, Bruzzo C, Cavalieri Z, Forlani A, Utkin Y, Casciano I, Romani M (2011). Inhibition of the nicotinic acetylcholine receptors by cobra venom α-neurotoxins: Is there a perspective in lung cancer treatment? PLoS One 6 (6), e20695
  20. Konshina AG, Boldyrev IA, Utkin YN, Omelkov AV, Efremov RG (2011). Snake cytotoxins bind to membranes via interactions with phosphatidylserine head groups of lipids. PLoS One 6 (4), e19064
  21. Osipov , Utkin  (2011). Phospholipase A2 and Signaling Pathways in Pheochromocytoma PC12 Cells.  (0),
  22. Tsetlin V, Utkin Y, Kasheverov I (2009). Polypeptide and peptide toxins, magnifying lenses for binding sites in nicotinic acetylcholine receptors. Biochem Pharmacol 78 (7), 720–731
  23. Mordvintsev DY, Polyak YL, Rodionov DI, Jakubik J, Dolezal V, Karlsson E, Tsetlin VI, Utkin YN (2009). Weak toxin WTX from Naja kaouthia cobra venom interacts with both nicotinic and muscarinic acetylcholine receptors. FEBS J 276 (18), 5065–5075
  24. Kasheverov IE, Utkin YN, Tsetlin VI (2009). Naturally occurring and synthetic peptides acting on nicotinic acetylcholine receptors. Curr Pharm Des 15 (21), 2430–2452
  25. Osipov AV, Kasheverov IE, Makarova YV, Starkov VG, Vorontsova OV, Ziganshin RK, Andreeva TV, Serebryakova MV, Benoit A, Hogg RC, Bertrand D, Tsetlin VI, Utkin YN (2008). Naturally occurring disulfide-bound dimers of three-fingered toxins: A paradigm for biological activity diversification. J Biol Chem 283 (21), 14571–14580
  26. Koval LM, Zverkova AS, Grailhe R, Utkin YN, Tsetlin VI, Komisarenko SV, Skok MV (2008). Nicotinic acetylcholine receptors alpha4beta2 and alpha7 regulate myelo- and erythropoiesis within the bone marrow. Int J Biochem Cell Biol 40 (5), 980–990
  27. Utkin YN, Osipov AV (2007). Non-lethal polypeptide components in cobra venom. Curr Pharm Des 13 (28), 2906–2915
  28. Lyukmanova EN, Shenkarev ZO, Schulga AA, Ermolyuk YS, Mordvintsev DY, Utkin YN, Shoulepko MA, Hogg RC, Bertrand D, Dolgikh DA, Tsetlin VI, Kirpichnikov MP (2007). Bacterial expression, NMR, and electrophysiology analysis of chimeric short/long-chain α-neurotoxins acting on neuronal nicotinic receptors. J Biol Chem 282 (34), 24784–24791
  29. Tsetlin V, Shelukhina I, Kryukova E, Burbaeva G, Starodubtseva L, Skok M, Volpina O, Utkin Y (2007). Detection of α7 nicotinic acetylcholine receptors with the aid of antibodies and toxins. Life Sci II 80 (2425), 2202–2205
  30. Mordvintsev DY, Rodionov DI, Makarova MV, Kamensky AA, Levitskaya NG, Ogay AY, Rzhevsky DI, Murashev AN, Tsetlin VI, Utkin YN (2007). Behavioural effects in mice and intoxication symptomatology of weak neurotoxin from cobra Naja kaouthia. Basic Clin Pharmacol Toxicol 100 (4), 273–278
  31. Kasheverov IE, Zhmak MN, Vulfius CA, Gorbacheva EV, Mordvintsev DY, Utkin YN, Van Elk R, Smit AB, Tsetlin VI (2006). α-Conotoxin analogs with additional positive charge show increased selectivity towards Torpedo californica and some neuronal subtypes of nicotinic acetylcholine receptors. FEBS J 273 (19), 4470–4481
  32. Tsetlin VI, Kasheverov IE, Zhmak MN, Utkin YN, Vulfius CA, Smit AB, Bertrand D (2006). α-conotoxin analogs with enhanced affinity for nicotinic receptors and acetylcholine-binding proteins. J Mol Neurosci 30 (12), 77–78
  33. Kasheverov IE, Chiara DC, Zhmak MN, Maslennikov IV, Pashkov VS, Arseniev AS, Utkin YN, Cohen JB, Tsetlin VI (2006). α-conotoxin GI benzoylphenylalanine derivatives 1H-NMR structures and photoaffinity labeling of the Torpedo californica nicotinic acetylcholine receptor. FEBS J 273 (7), 1373–1388
  34. Feofanov AV, Sharonov GV, Astapova MV, Rodionov DI, Utkin YN, Arseniev AS (2005). Cancer cell injury by cytotoxins from cobra venom is mediated through lysosomal damage. Biochem J 390 (1), 11–18
  35. Dubovskii PV, Lesovoy DM, Dubinnyi MA, Konshina AG, Utkin YN, Efremov RG, Arseniev AS (2005). Interaction of three-finger toxins with phospholipid membranes: Comparison of S- and P-type cytotoxins. Biochem J 387 (3), 807–815
  36. Osipov AV, Levashov MY, Tsetlin VI, Utkin YN (2005). Cobra venom contains a pool of cysteine-rich secretory proteins. Biochem Biophys Res Commun 328 (1), 177–182
  37. Shoibonov BB, Osipov AV, Kryukova EV, Zinchenko AA, Lakhtin VM, Tsetlin VI, Utkin YN (2005). Oxiagin from the Naja oxiana cobra venom is the first reprolysin inhibiting the classical pathway of complement. Mol Immunol 42 (10), 1141–1153
  38. Feofanov AV, Sharonov GV, Dubinnyj MA, Astapova MV, Kudelina IA, Dubovskij PV, Rodionov DI, Utkin YN, Arseniev AS (2004). Comparative study of structure and activity of cytotoxins from venom of the cobras Naja oxiana, Naja kaouthia, and Naja haje. Biochemistry (Mosc) 69 (10), 1410–1421
  39. Osipov AV, Astapova MV, Tsetlin VI, Utkin YN (2004). The first representative of glycosylated three-fingered toxins: Cytotoxin from the Naja kaouthia cobra venom. 271 (10), 2018–2027
  40. Skok MV, Kalashnik EN, Koval LN, Tsetlin VI, Utkin YN, Changeux JP, Grailhe R (2003). Functional nicotinic acetylcholine receptors are expressed in B lymphocyte-derived cell lines. Mol Pharmacol 64 (4), 885–889
  41. Dubovskii PV, Lesovoy DM, Dubinnyi MA, Utkin YN, Arseniev AS (2003). Interaction of the P-type cardiotoxin with phospholipid membranes. 270 (9), 2038–2046
  42. Kasheverov IE, Zhmak MN, Maslennikov IV, Utkin YN, Tsetlin VI (2003). A comparative study on selectivity of α-conotoxins GI and ImI using their synthetic analogues and derivatives. Neurochem Res 28 (34), 599–606
  43. Oustitch TL, Peters LE, Utkin YN, Tsetlin VI (2003). Direct cloning of a target gene from a pool of homologous sequences: Complete cDNA sequence of a weak neurotoxin from cobra Naja kaouthia. IUBMB Life 55 (1), 43–47
  44. Kasheverov IE, Rozhkova AM, Zhmak MN, Utkin YN, Tsetlin VI (2002). Photoactivatable analogues of α-conotoxins GI and MI and their interaction with nicotinic acetylcholine receptor. Bioorg Khim 28 (2), 107–108
  45. Osipov AV, Starkov VG, Utkin YN (2002). Toxicity of cobra venom components to cockroach Gromphadorhina portentosa. Toxicon 40 (10), 1507–1509
  46. Utkin YN, Kasheverov IE, Tsetlin VI (2001). α-Neurotoxins and α-conotoxins, blockers of nicotinic acetylcholine receptors. Bioorg Khim 25 (11), 805–810
  47. Rzhevskii DI, Murashev AN, Kukhtina VV, Tsetlin VI, Utkin YN (2001). The weak neurotoxin from the Naja kaouthia cobra venom decreases the arterial blood pressure in rats. Bioorg Khim 27 (3), 223
  48. Zhmak MN, Kasheverov IE, Utkin YN, Tsetlin VI, Volpina OM, Ivanov VT (2001). An efficient synthetic scheme for natural α-conotoxins and their analogues. Bioorg Khim 27 (2), 87–88
  49. Utkin YN, Dubovskii PV, Dubinnyi MA, Zharavin VA, Simonova TN, Barsukov LI, Arseniev AS (2001). The Naja oxiana venom cytotoxin II spin-labeled at Lys35 for the EPR study of its interaction with phospholipid membranes. Bioorg Khim 25 (12), 930–932
  50. Kasheverov I, Rozhkova A, Zhmak M, Utkin Y, Ivanov V, Tsetlin VI (2001). Photoactivatable α-conotoxins reveal contacts with all subunits as well as antagonist-induced rearrangements in the Torpedo californica acetylcholine receptor. 268 (13), 3664–3673
  51. Utkin YN, Kukhtina VV, Kryukova EV, Chiodini F, Bertrand D, Methfessel C, Tsetlin VI (2001). "Weak Toxin" from Naja kaouthia is a Nontoxic Antagonist of α7 and Muscle-type Nicotinic Acetylcholine Receptors. J Biol Chem 276 (19), 15810–15815
  52. Dubinnyi MA, Dubovskii PV, Utkin YN, Simonova TN, Barsukov LI, Arseniev AS (2001). An ESR study of the cytotoxin II interaction with model membranes. Bioorg Khim 27 (2), 112–113
  53. Osipov AV, Weise C, Franke P, Kukhtina VV, Frings S, Hucho F, Tsetlin VI, Utkin YN (2001). Cobra venom contains a protein of the CRISP family. Bioorg Khim 27 (3), 225–226
  54. Dubovskii PV, Dementieva DV, Bocharov EV, Utkin YN, Arseniev AS (2001). Membrane binding motif of the P-type cardiotoxin. J Mol Biol 305 (1), 137–149
  55. Kukhtina VV, Weise C, Osipov AV, Starkov VG, Titov MI, Esipov SE, Ovchinnikova TV, Tsetlin VI, Utkin YN (2000). The MALDI mass spectrometry in the identification of new proteins in snake venoms. Bioorg Khim 26 (11), 803–807
  56. Utkin YN, Tsetlin VI, Hucho F (2000). Structural organization of nicotinic acetylcholine receptors. Cancer Biol Med 13 (2), 143–164
  57. Utkin YN (2000). Photoactivatable derivatives of peptide and protein ligands in the study of neuroreceptor structure. Bioorg Khim 26 (1), 23–24
  58. Kukhtina VV, Weise C, Muranova TA, Starkov VG, Franke P, Hucho F, Wnendt S, Gillen C, Tsetlin VI, Utkin YN (2000). Muscarinic toxin-like proteins from cobra venom. 267 (23), 6784–6789
  59. Alexeev T, Krivoshein A, Shevalier A, Kudelina I, Telyakova O, Vincent A, Utkin Y, Hucho F, Tsetlin V (1999). Physicochemical and immunological studies of the N-terminal domain of the Torpedo acetylcholine receptor α-subunit expressed in Escherichia coli. 259 (12), 310–319
  60. Krivoshein AV, Kudelina IA, Alexeev TA, Shevalier AF, Utkin YN, Tsetlin VI (1998). The Secondary Structure of Some α-Subunit Fragments of Torpedo californica Acetylcholine Receptor. Bioorg Khim 24 (12), 931–932
  61. Lugovskoy AA, Maslennikov IV, Utkin YN, Tsetlin VI, Cohen JB, Arseniev AS (1998). Spatial structure of the M3 transmembrane segment of tire nicotinic acetylcholine receptor α subunit. 255 (2), 455–461
  62. Utkin YN, Krivoshein AV, Davydov VL, Kasheverov IE, Franke P, Maslennikov IV, Arseniev AS, Hucho F, Tsetlin VI (1998). Labeling of Torpedo californica nicotinic acetylcholine receptor subunits by cobratoxin derivatives with photoactivatable groups of different chemical nature at Lys23. 253 (1), 229–235
  63. Kasheverov IE, Utkin YN, Tsetlin VI (1997). Antagonists of the Tachykinin Receptors. Bioorg Khim 23 (6), 464–468
  64. Dementeva DV, Utkin YN, Arseniev AS (1996). Secondary Structure and Conformational Heterogeneity of Cytotoxin II from Naja naja oxiana. Bioorg Khim 22 (5), 351–352
  65. Machold J, Utkin Y, Kirsch D, Kaufmann R, Tsetlin V, Hucho F (1995). Photolabeling reveals the proximity of the α-neurotoxin binding site to the M2 helix of the ion channel in the nicotinic acetylcholine receptor. Proc Natl Acad Sci U S A 92 (16), 7282–7286
  66. Machold J, Weise C, Utkin Y, Tsetlin V, Hucho F (1995). The Handedness of the Subunit Arrangement of the Nicotinic Acetylcholine Receptor from Torpedo californica. FEBS J 234 (2), 427–430
  67. GOLOVANOV AP, LOMIZE AL, ARSENIEV AS, UTKIN YN, TSETLIN VI (1993). Two‐dimensional 1H‐NMR study of the spatial structure of neurotoxin II from Naja naja oxiana. FEBS J 213 (3), 1213–1223
  68. Kreienkamp HJ, Weise C, Hucho F, Utkin YN, Machold J, Tsetlin VI (1992). Investigation of Ligand-Binding Sites of the Acetylcholine Receptor Using Photoactivatable Derivatives of Neurotoxin II from Naja naja oxiana. Biochemistry 31 (35), 8239–8244
  69. Utkin YN, Lazakovich EM, Kasheverov IE, Tsetlin VI (1989). α-Bungarotoxin interacts with the rat brain tachykinin receptors. FEBS Lett 255 (1), 111–115
  70. Kaydalov AA, Utkin YN, Andronova TM, Tsetlin VI, Ivanov VT (1989). Muramyl peptides bind specifically to rat brain membranes. FEBS Lett 248 (12), 78–82
  71. Arseniev AS, Utkin YN, Pashkov VS, Tsetlin VI, Ivanov VT, Bystrov VF, Ovchinnikov YA (1981). 19F NMR determination of intramolecular distances in spin- and fluorine-labelled proteins. Neurotoxin II Naja naja oxiana. FEBS Lett 136 (2), 269–274
  72. Tsetlin VI, Karlsson E, Arseniev AS, Utkin YN, Surin AM, Pashkov VS, Pluzhnikov KA, Ivanov VT, Bystrov VF, Ovchinnikov YA (1979). EPR And fluorescence study of interaction of Naja naja oxiana neurotoxin II and its derivatives with acetylcholine receptor protein from Torpedo marmorata. FEBS Lett 106 (1), 47–52
  73. ARSENIEV AS, BALASHOVA TA, UTKIN YN, TSETLIN VI, BYSTROV VF, IVANOV VT, OVCHINNIKOV YA (1976). Proton‐Nuclear‐Magnetic‐Resonance Study of the Conformation of Neurotoxin II from Middle‐Asian Cobra (Naja naja oxiana) Venom. FEBS J 71 (2), 595–606