Transcriptomic Analysis of TDP1-Knockout HEK293A Cells Treated with the TDP1 Inhibitor (Usnic Acid Derivative)
Tyrosyl-DNA phosphodiesterase 1 (TDP1) is a key enzyme for the repair of stalled topoisomerase 1 (TOP1)-DNA complexes. Previously, we obtained HEK293A cells with homozygous knockout of the gene by the CRISPR/Cas9 method and used them as a cell model to study the mechanisms of anticancer therapy and to investigate the effect of gene knockout on gene expression changes in the human HEK293A cell line by transcriptome analysis. In this study, we investigated the effect of a TDP1 inhibitor ((R,E)-2-acetyl-6-(2-(2-(4-bromobenzyliden) hydrazinyl) thiazol-4-yl)-3,7,9-trihydroxy-8,9b-dimethyldibenzo[b,d] furan-1(9bH)-one, OL9-119, an usnic acid derivative), capable of potentiating the antitumor effect of topotecan, as well as its combination with topotecan, on the transcriptome of wild-type and TDP1 knockout HEK293A cells. OL9-119 was found to be able to reduce cell motility by decreasing the expression of a number of genes, which may explain the antimetastatic effect of this compound. Differentially expressed genes (DEGs) related to electron transport, mitochondrial function, and protein folding were also identified under TDP1 inhibitor treatment.
Zakharenko AL, Dyrkheeva NS, Markov AV, Kleshchev MA, Ryabchikova EI, Malakhova AA, Orishchenko KE, Okorokova LS, Shtokalo DN, Medvedev SP, Zakian SM, Tupikin AA, Kabilov MR, Luzina OA, Deyev SM, Lavrik OI
Нет данных о цитировании
Данные статьи проверены модераторами 2025-10-23
Список научных проектов, где отмечена публикация
- Oncotheranostics and problems of resistance to antitumor and antibacterial drugs (April 23, 2024 — December 31, 2026). Deyev S.M., Alekseeva N.A., Alekseeva L.G., Aliper E.T., Alferova V.A., Andrianova A.G., Babkova Y.S., Baranova A.A., Baranova M.N., Belogurov A.A., Bogachuk A.P., Bocharov E.V., Burenina O.Y., Bychkov M.L., Veretenenko I.I., Volkov D.V., Gabibov A.G., Gordeeva V.D., Gorelik L.V., Gryaznova O.Y., Gulyak E.L., Deryabin A.S., Dobrochaeva K.L., Evpak A.S., Egorova N.S., Emel'yanov E.A., Ermolaeva E.O., Efremov R.G., Zhitlov M.Y., Zhuchkov V.A., Zakharova M.Y., Zvyagin A.V., Zelepukin I.V., Zinchenko A.A., Kalganova A.I., Kirichenko A.V., Kirpichnikov M.P., Knorre V.D., Kovaleva D.K., Kovalenko E.I., Kokaeva I.S., Konovalova E.V., Konshina A.G., Korotkova N.A., Korshun V.A., Kotel'nikova O.V., Kotelnikova P.A., Kravchenko T.V., Krylov N.A., Kudriaeva A.A., Kuzmina O., Kurbatskaya I.N., Lazarev I.V., Logvinov A.S., Lyukmanova E.N., Mazur R.V., Mallaev R.A., Mirzoeva N.Z., Mirkasymov A.B., Musatova O.E., Nechaeva A.M., Nikitin T.D., Nikonova A.V., Nolde D.E., Odzhomoko L.O., Orlova D.A., Panina I.S., Pakhomov A.A., Pahomova T.E., Pizhonkov N.A., Pipiya S.O., Polyansky A.A., Pohorukov D.A., Prokopenko Y.A., Proshkina G.M., Rodionov V.I., Rotanova T.V., Rubcov Y.P., Ryabukha Y.A., Savateeva N.S., Sapozhnikova K.A., Serova E.V., Sinichich A.A., Smirnov I.V., Sogomonyan A.S., Spallone A., Stepanova (Ukrainskaya) V.M., Streltsova M.A., Sutugin Y.A., Terekhov S.S., Frolova A.Y., Khavroshechkina E.V., Khodarovich Y.M., Kholodenko R.V., Tsetlin V.I., Chumakov S.P., Shilova P.A., Shlepova O.V., Shramova E.I., Shul'ga A.A.. Grant, Budget.
: 
: 41096560