Быстров Владимир Фёдорович

Образование

Период обученияСтрана, городУчебное заведениеДополнительная информация
1953–1959 Россия, Москва Московский государственный университет им. М.В. Ломоносова (МГУ), физический факультет Диплом физика
1964 Россия, Москва Институт химической физики АН СССР (ИХФ) Присуждена учёная степень кандидата физико-математических наук за диссертацию "Спектроскопия ядерного магнитного резонанса циклических соединений"
1973 Россия, Москва Присуждена учёная степень доктора физико-математических наук за диссертацию "ЯМР-исследование пространственного строения биологически важных соединений"

Премии и заслуги

  • Юбилейная медаль «За доблестный труд. В ознаменование 100-летия со дня рождения Владимира Ильича Ленина» (1970);
  • Орден "Знак Почёта" - за заслуги в развитии советской науки и в связи с 250-летием Академии наук СССР (1975);
  • Орден Дружбы народов - за достигнутые успехи в выполнении заданий X пятилетки по развитию науки и техники, внедрению результатов исследований в народное хозяйство (1981);
  • Орден Трудового Красного Знамени - за заслуги в развитии биоорганической химии, подготовке научных кадров и в связи с пятидесятилетием со дня рождения (1985);
  • Государственная премия СССР в области науки и техники - за цикл работ "Нейротоксины как инструменты исследования молекулярных механизмов генерации нервного импульса", опубликованных в 1973-1983 гг. (совместно с В.Т. Ивановым, Е.В. Гришиным, В.И. Цетлиным) (1985);
  • Золотая медаль Я. Гейровского Чехословацкой академии наук - за заслуги в области химических наук (1988);
  • Премия РАН имени М.М. Шемякина за цикл работ "Исследование структуры и функции мембранных пептидов и белков методом спектроскопии ЯМР" (совместно с А.С. Арсеньевым) (1992, посмертно)

Избранные публикации

  1. Arseniev A.S., Bystrov V.F., Lomize A.L., Ovchinnikov Yu.A. (1985). 1H-NMR study of gramicidin A transmembrane ion channel. Head-to-head right-handed, single-stranded helices. FEBS Lett. 186 (2), 168–174 [+]

    The structure of [Val1] gramicidin A incorporated into sodium dodecyl-d25 sulphate micelles has been studied by two-dimensional proton NMR spectroscopy. Analysis of nuclear Overhauser effects, spin-spin couplings and solvent accessibility of NH groups show that the conformation of the Na+ complex of gramicidin A in detergent micelles, which in many ways mimic the phospholipid bilayer of biomembranes, is an N-terminal to N-terminal (head-to-head) dimer Image formed by two right-handed, single-stranded β6.3 helices with 6.3 residues per turn, differing from Urry's structure by handedness of the helices.

    ID:144
  2. Ovchinnikov Yu.A., Bystrov V.F., Ivanov V.T. (1984). NMR solution conformation of gramicidin A double helix. FEBS Lett. 165 (1), 51–56 [+]

    The conformation of species 3 of Val-gramicidin A in dioxane has been determined by two-dimensional NMR spectroscopy. It is presented by the left handed up arrow, down arrowππ5.6LD double helix, a suitable model of an ion permeable pore across the membrane matrix.

    ID:143
  3. Bystrov V.F., Gavrilov Yu.D., Ivanov V.T., Ovchinnikov Yu.A. (1977). Refinement of the solution conformation of valinomycin with the aid of coupling constants from thr 13C-nuclear-magnetic-resonance spectra. Eur. J. Biochem. 78 (1), 63–82 [+]

    he C'= O and Cα signals in the 13C nuclear magnetic resonance (NMR) spectra of valinomycin have been assigned and the vicinal 1H…13C coupling constants have been determined by double and triple heteronuclear resonance. In conjunction with the vicinal H-NCα-H and H-CαCβ-H proton-proton constants, the results led to unequivocal determination of the torsion angles φ and of the population distribution of the Cα-Cβ rotational states. The Φ torsion angles for the hydroxy acid residues were estimated from the vicinal 1H-CαC'-15N constants. The combined data permitted refinement of the conformational states of valinomycin in different solvents. For the KS+ complex of valinomycin the observed couplings are in complete accord with the conformations we had earlier proposed for solutions and that had also been established by X-ray analysis. In the 13C spectra of the valinomycin-Tl+ complex 13C…203,205Tl+ spin-spin couplings were observed for the l and d-valine carbonyls, unequivocal proof of the donor-acceptor interaction with the cation. In media of weak polarity (cyclohexane, chloroform) the conformation of the valinomycin molecule is similar to that of the K+ complex. In such a 'bracelet' structure formed by six fused β-turns of type II and II', the amino acid carbonyls are axial with certain inclination towards the symmetry axis. On formation of a 1:1 complex with a cation the carbonyl orientation changes, now bending towards the center of the molecular cavity. In the 'propeller' conformation, predominant in solvents of medium polarity (for instance CCl4/(C2H3)2SO, 3/1) the three β-turns are of type II. The 1H and 13C chemical shifts are interpreted in terms of conformational changes in the valinomycin molecule, intermolecular and intramolecular hydrogen bonds and interaction with the metal cation.

    ID:139
  4. Bystrov V.F., Ivanov V.T., Portnova S.L., Balashova T.A., Ovchinnikov Yu.A. (1973). Refinement of the angular dependence of the peptide vicinal NH-CaH coupling constant. Tetrahedron 29 (6), 873–877 [+]

    The refined dependence of the peptide NHCαH vicinal coupling constant on the dihedral angle θ have been derived on the basis of the accumulated experimental data. The mean permissible values (in Hz) are approximated by 3JNHCH = 9·4 cos2 θ - 1·1 cos θ + 0·4 An analogous relationship for the sum of two vicinal NH-CαH2 coupling constants in the glycyl residue have been calculated from the above dependence. Measurements on N-methylacetamide in various solvents and in the presence of an alkali salt showed the vicinal constant NH-CH to vary by not more than ± 3%. Some of the other proposed 3JNHCH(θ) dependencies give too low values for the cis-oriented NH and CαH bonds. This may be due to the fact that in these correlations the data for compounds with cis-amide bonds have been used for 0° - θ - 90° region of the dependence.

    ID:1044
  5. Bystrov V.F., Dubrovina N.I., Barsukov L.I., Bergelson L.D. (1971). NMR differentiation of the internal and external phospholipid membrane surfaces using paramagnetic Mn2+ and Eu3+ ions. Chem. Phys. Lipids 6 (4), 343–350 [+]

    The effect of paramagnetic Mn2+ and Eu3+ ions on the NMR spectra of sonicated lecithin dispersions in water and benzene has been investigated. In aqueous dispersions containing paramagnetic ions the N+(CH3)3 signal of the lecithin molecules in the lipid bilayer consists of two components, one broadened by Mn2+ and shifted to high field by Eu3+ must correspond to molecules in the external surface in contact with the bulk salt solution, and the other corresponding to internal lecithin molecules in contact with the ion free internal aqueous phase. Thus the external and internal surfaces of a lecithin containing membrane can be discriminated. The rates of exchange of the paramagnetic ions, of the lecithin and the water molecules between micelles and/or between micelles and external medium have been evaluated.

    ID:1045