Artificial Climate Station “BIOTRON”

Department of Plant Molecular Biology and Biotechnology

Head: Sergey Dolgov, D.Sc
dolgov@bibch.ru+7(4967)73-06-53#3237, +7(3237)73-17-79#3126

The subdivision is one of the leading Russian laboratories, successfully carrying out research in various areas of plant biotechnology. The employees of the Laboratory face the task of obtaining priority scientific results in the field of molecular biology, the functioning and organization of the plant genome, the creation of biotech forms of new-generation plants for fundamental and applied research that are designed to ensure the development of Russia's agro-industrial complex and promote the development of the production of medical and veterinary substances using plant-producers (bio-factories).

For more than 20 years, the Laboratory has been conducting research on the development of agrobacterial and ballistic methods for the production of transgenic plants of fruit, berry, vegetable, cereals, ornamental crops with new economically valuable traits. During this time, the Laboratory staff received more than a thousand independent transgenic plants from such important agricultural crops as wheat, tomato, apple, pear, plum, carrot, beet, etc. Work is underway to clone plant genes and create modern expression systems and genetic structures for successful modification of genomes of cultivated plants. Since 2000, the Laboratory staff has been conducting field trials of transgenic plants, many of these studies were performed for the first time not only in Russia, but also in the world.

The laboratory operates on the basis of a unique scientific installation (USI) "Station for working out methods for obtaining transgenic plants and ballistic transformation of plant objects".

Selected publications (show all)

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Sergey Dolgov

  • Russia, Moscow, Ul. Miklukho-Maklaya 16/10 — On the map
  • IBCh RAS, build. ФИБХ, office. Лаб-1/201-4
  • Phone: +7(4967)73-06-53#3237
  • E-mail: dolgov@bibch.ru

The test on laboratory animals of the recombinant vaccine protein M2e-RTB, obtained in the duckweed plants. (2018-11-30)

In accordance with the research plan in 2018, a molecular-biological analysis of duckweed plants was carried out; the duckweed lines, producers of recombinant vaccine protein M2e-RTB, were obtained for the first time. The recombinant protein M2e-RTB obtained in plants of the duckweed was orally immunized to mice, and induction of antibodies to the peptide M2e of the avian influenza virus was shown.

"Development of systems for the synthesis of recombinant proteins based on plant expression platforms (biofarming)". № 01201352434

Study of molecular biological mechanisms and the development of molecular breeding methods for obtaining plants of agricultural crops that are resistant to environmental stress factors and with improved crop quality (2018-11-30)

An analysis of the inheritance of transgenic traits in the offspring of 54 primary transgenic lines of T0 common wheat, containing the genes for resistance to the herbicide bar and salt stress hvnxh2 or agnhx, was carried out. 165 T2-T3 populations inheriting stress resistance genes as homozygous alleles were identified. Molecular and biological analysis confirmed the expression of salt tolerance genes in the homozygous progeny of 16 T0 lines. To study the post-transcriptional regulation of the ripening time of fruits, 27 transgenic apple lines adapted to ex vitro conditions, which contain expression cassettes to suppress ethylene synthesis, were successfully grafted onto rootstocks in order to accelerate their flowering and fruiting.

Study of molecular biological mechanisms and the development of molecular breeding methods for obtaining plants of agricultural crops that are resistant to environmental stress factors and with improved crop quality. № 0101-2014-0047, № 01201352435