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Lecture by Prof. Zhang Shuguang «The QTY Code: A tool for designing detergent-free membrane proteins»

Prof. Zhang Shuguang from MIT will deliver a lecture entitled «The QTY Code: A tool for designing detergent-free membrane proteins».

Date and time: Mon 24 December 2018 15:00. Location: Conference hall at 3rd floor BON IBCh.

December 24, 2018 (This event is over)

membrane proteins, spatial structure, GPCR, detergent, QTY code

Goncharuk Sergey

Structure and function studies of membrane proteins, particularly G protein-coupled receptors and multipass transmembrane proteins, require detergents. We have devised a simple tool, the QTY code (glutamine, threonine, and tyrosine), for designing hydrophobic domains to become water soluble without detergents. Here we report using the QTY code to systematically replace the hydrophobic amino acids leucine, valine, isoleucine, and phenylalanine in the seven transmembrane α-helices of CCR5, CXCR4, CCR10, and CXCR7. We show that QTY code-designed chemokine receptor variants retain their thermostabilities, α-helical structures, and ligand-binding activities in buffer and 50% human serum. CCR5QTY, CXCR4QTY, and CXCR7QTY also bind to HIV coat protein gp41-120. Despite substantial transmembrane domain changes, the detergent-free QTY variants maintain stable structures and retain their ligand-binding activities. We believe the QTY code will be useful for designing water-soluble variants of membrane proteins and other water-insoluble aggregated proteins.

december 10, 2018