Sergey A. Goncharuk

ph. d. (biological sciences)

Phone: +7 (495) 335-27-33, +7 (495) 330-74-83,

Education

Period	Country, city	Education institution	Additional info
1999–2005	Russia, Dolgoprudniy	Moscow Institute of Physics and Technology (State University) (MIPT)	Bachelor. Master's degree.
2002–2005	Russia, Moscow	M.M. Shemyakin and Yu.A. Ovchinnikov Institute of bioorganic chemistry, Educational and Scientific Center
2005–2008	Russia, Dolgoprudniy	Moscow Institute of Physics and Technology (State University) (MIPT)	Ph.D student.
2008	Russia, Moscow	Moscow State University, biological faculty, department of bioengineering	Ph.D. in biology (biophysics).

FEBS

Goncharuk S.A., Shulga A.A., Ermolyuk Y.S., Kuzmichev P.K., Sobol V.A., Bocharov E.V., Chupin V.V., Arseniev A.S., Kirpichnikov M.P. (2009). Bacterial synthesis, purification, and solubilization of membrane protein KCNE3, a regulator of voltage-gated potassium channels. Biochemistry Mosc. 74 (12), 1344–9 [+]
An efficient method is described for production of membrane protein KCNE3 and its isotope labeled derivatives ((15)N-, (15)N-/13C-) in amounts sufficient for structural-functional investigations. The purified protein preparation within different detergent micelles was characterized using dynamic light scattering, CD spectroscopy, and NMR spectroscopy. It is shown that within DPC/LDAO micelles the protein is in monomeric form and acquires mainly alpha-helical conformation. The existence of cross-peaks for all glycines of the (15)N-HSQC NMR spectra as well as relatively small line widths (~20 Hz) confirm the high quality of the preparation and the possibility of obtaining structural-dynamic information on KCNE3 by high resolution heteronuclear NMR spectroscopy.