Science-Educational center

Science-Educational  center

Scientific units

Head: Tatiana Ovchinnikova, D.Sc, professor
ovch@ibch.ru+7(495)336-44-44, +7(495)995-55-57#2062

Subject of the research:“Construction of new-age medicines on basis of peptide antibiotics — molecular factors of innate immunity”
Leader of the research: the director of ESC T.V. Ovchinnikova.

At the moment medicine faces with a problem of pathogens’ resistance to antibiotics. Intensive rising of resistant bacterial infections makes search of new medicines be necessary. Research at the SEC IBCh are targeted to a complex structural and functional study of new natural peptide antibiotics, mechanisms of their functioning as molecular factors of inherent immunity, to development of production technologies of their recombinant and synthetic analogs, to preclinical tests and design on their base of new-age antibiotics.

Discovery of versatile biological functions of endogenous antimicrobial peptides (AMP) shows that these natural antibiotics are perspective molecules for creation of new antibiotics and have ability to inactivate a wide spectrum of microorganisms, including bacteria, fungi, protozoa, tunicary viruses. AMP as molecular factors of inherent immunity work as mediators of phagocytic, inflammatory and stress processes. In view of AMPs’ antibacterial and immunomodulatory activity presence, a lot of foreign pharmaceutical companies (in USA, Canada, France, the Netherlands, etc.) began creation of new antibiotics on their base. Successful clinical trials were passed by peptide antibiotics for treatments of sepsis, meningitis, pneumonia, gastric ulcer, candidiasis, mucositis, gingivitis, acne, impetigo and for healing of burns and wounds complicated with repeated infection.

Research at the ESC are aimed to discovery, purification, study of structure, biological characteristics and molecular mechanisms of natural peptide antibiotics’ action, development of methods of their production and to preclinical tests. Objects of research are bacterial, fungi, vegetable and animal AMPs, including those of bacteria Bаcillus licheniformis, fungi Emericellopsis salmocynnemata and Emericellopsis minima, lentil Lens culinaris, nereides Arenicola marina, jellyfish Aurelia aurita, sturgeon Acipencer guldenstadti, tortoise Emys orbicularis, toads Bufo bufo gargarisan, etc. Particularly, from coelomic cell of nereides Arenicola marina new peptides were extracted, which have high antibacterial activity in relation to gram-positive and gram-negative bacteria and yeast fungi; they were named “arenicins”. Determined by us full primary structure of processed arenicins and genes of their predecessors lets come to the conclusion that we revealed new family peptides, which don’t belong to any known family of AMP. A new effective technology of a heterologous expression was created, strain-producers were engineered, new methods of extraction and purification of genetically engineered arenicins were developed. Few patents of Russian Federation were acquired for original structure of arenicins and their production methods. At the same time fundamental research on arenicins activities are being continued, so as detailed study of their biological activity mechanisms and preclinical tests with animal models. Discovery, structural and functional research of other groups of peptide antibiotics are being continued too. The matters being under the study are original and patentable. The final aim of project is creation of new effective medicines’ prototypes series on base natural peptide antibiotics and biotechnological platform for their production.

NamePositionContacts
Tatiana Ovchinnikova, D.Sc, professordept. headovch@ibch.ru+7(495)336-44-44, +7(495)995-55-57#2062
Lev Patrushev, D.Sc, professorl. r. f.levpatrushev0@gmail.com
Sergey Balandin, Ph.D.s. r. f.serb@ibch.ru+7(495)335-09-00
Ekaterina Finkina, Ph.D.s. r. f.finkina@mail.ru+7(495)335-42-00
Ol'ga Shamborants. r. f.osha@ibch.ru+7(495)336-46-00
Elena Stukacheva, Ph.D.s. r. f.east@ibch.ru+7(495)336-46-00
Sergej Sychyov, Ph.D.s. r. f.svs@ibch.ru+7(495)335-66-22
Andrej Tagaevs. r. f.andtag@ibch.ru+7(495)335-42-00
Ivan Bogdanov, Ph.D.r. f.contraton@mail.ru+7(495)335-42-00
Ilia Bolosovr. f.bolosoff@gmail.com+7(495)3354200
Svetlana Guryanova, Ph.D.r. f.svgur@mail.ru
Daria Melnikova, Ph.D.r. f.d_n_m_@mail.ru+7(495)3354200
Pavel Panteleev, Ph.D.r. f.alarm14@gmail.com+7(495)335-09-00
Ekaterina Aleshinasen. eng.educ@bibch.ru
Galina Boikosen. eng.asping@ibch.ru+7(495)330-24-00
Dmitriy Esipovsen. eng.desipov@gmail.com
Julija Leonovasen. eng.leonovajuf@yandex.ru+7(495)335-09-00
Tat'jana Simonovasen. eng.tsimonova@ibch.ru+7(495)335-66-22
Leona Snezhkova, Ph.D.sen. eng.cell6370@yandex.ru+7(495)335-66-22
Stanislav Sukhanovsen. eng.ssv@ibch.ru+7(495)335-66-22
Natalija Svishevasen. eng.natasvi@mail.ru+7(495)335-42-00, +7(495)741-78-20
Daria Antoshinaeng.riruka11@mail.ru
Galina Chicherinaeng.galina.tchicherina@yandex.ru+7(495)336-46-00
Viktoria Safronovaeng.saf-victorianik@yandex.ru

All publications (show selected)

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Tatiana Ovchinnikova

  • Russia, Moscow, Ul. Miklukho-Maklaya 16/10 — On the map
  • IBCh RAS, build. 32, office. 610
  • Phone: +7(495)336-44-44
  • E-mail: ovch@ibch.ru
  • Fax: +7 (495) 336-43-33

The key amino acids of lentil Lc-LTP2 for the formation of a complexes with lipids were discovered. PI(4,5)P2 is a new signal ligand of Lc-LTP2.

Lipid transport proteins (LTPs) are involved in a number of processes in plants due to their ability to bind and transport a wide range of hydrophobic ligands. The spatial structure of LTP is characterized by the presence of an internal cavity that can accommodate hydrophobic ligands. The UC has shown for the first time that lentil Lc-LTP2, as well as LTP from other plants, bind phosphatidylinositol (4,5) -bisphosphate (PI (4,5) P2) and, possibly, participate in signal transduction. It was shown for the first time that two amino acid residues conserved for plant LTPs, Arg45 and Tyr80, play an important role both in initiating interactions with various ligands and in the stabilization of protein-ligand complexes. It has also been shown that Tyr80 plays a key role in the interaction of Lc-LTP2 with the membrane.

Novel β-hairpin BRICHOS domain-related antimicrobial peptides from the marine polychaeta Capitella teleta

In collaboration with Laboratory of structural biology of ion channels

The rapid growth of antibiotic resistance, combined with the difficulties of finding new antibiotics, requires fundamentally different approaches to the creation of anti-infectious drugs. As part of the study of BRICHOS-associated antimicrobial peptides (AMPs) of animals carried out at the Science-Educational Center  Recombinant analogs of new host defense peptides from the marine polychaete Capitella teleta were obtained, and their structural and biological properties, including the molecular mechanism action against bacteria were studied. Captellacins  are new BRICHOS-associated AMPs from the polychaete C. teleta, structurally similar to tachyplesins, the host defense peptides of horseshoe crabs. The relatively low  amphiphilicity determines the low cytotoxicity of captellacins in relation to normal mammalian cells. Using high-resolution NMR spectroscopy, the spatial structure was determined , and the membrane activity of the recombinant analogue of the antimicrobial peptide capitellacin from the marine worm Capitella teleta was studied. Captellacin  in aqueous solution is presented as a monomer and forms a right-handed beta-hairpin. Its structure does not possess the amphipathicity typical of peptides homologous to it, but it retains its ability to permeate the membrane. Probably, due to the lack of a pronounced amphipathicity, captellacin does not form dimers in model

 membranes and retains its conformation in the membrane environment. The study of the spatial structure and membrane activity of antimicrobial peptides opens the way to the development of new antibiotics based on them. Based on the results of the work, a patent of the Russian Federation (RU No. 2721273) was obtained and the article was published in the journal Marine Drugs (IF 4.073): P.V. Panteleev, A.V. Tsarev, V.N. Safronova, O.V. Reznikova, I.A. Bolosov, S.V. Sychev, Z.O. Shenkarev, T.V. Ovchinnikova. Structure Elucidation and Functional Studies of a Novel β-hairpin Antimicrobial Peptide from the Marine Polychaeta Capitella teleta. Marine Drugs, 2020, 18, 620; doi: 10.3390 / md18120620.

Novel antimicrobial peptides from leucocytes of the goat Capra hircus

As part of the study of antimicrobial peptides (AMPs) from animal species, conducting at the Science-Educational Centre of the IBCh RAS, biological properties of novel host-defense cationic peptides from leucocytes of the goat Capra hircus – α-helical peptide ChMAP-28 and proline-rich peptide mini-ChBac7.5Nα – were studied. The peptides were expressed in the bacterial system, and their molecular mechanism of action was analyzed. The marked synergistic effect against extensively drug-resistant bacteria was displayed by the host-defense cathelicidins from the goat. ChMAP-28 prevents bacterial resistance to ribosome-targeting proline-rich antimicrobial peptides. The marked activity of ChMAP-28 against cancer cells was shown, molecular mechanism of anticancer action of the peptide was also proposed. Two papers were published in Q1 journals and the patent of the Russian Federation was obtained.

Novel antimicrobial peptides from ancient marine invertebrates

In collaboration with Laboratory of structural biology of ion channels

As a part of the study of antimicrobial peptides (AMPs) from animal species, conducting at the Science-Educational Centre of the IBCh RAS, novel host-defense cationic peptides from ancient marine invertebrates were found – nicomicin-1 and -2 from the small Arctic polychaeta Nicomache minor and polyphemusin III from the horseshoe crab Limulus polyphemus, and their structural and biological properties were studied. The peptides were expressed in the bacterial system, and their spatial structure was analyzed. Nicomicins are unique among polychaeta AMPs scaffolds, combining an amphipathic N-terminal α-helix and C-terminal extended part with a six-residue loop stabilized by a disulfide bridge. This structural arrangement resembles C-terminal Rana-box motif observed in the α-helical host-defense peptides isolated from frog skin. Nicomicin-1 exhibited strong in vitro antimicrobial activity against Gram-positive bacteria at submicromolar concentrations. The main mechanism of nicomicin-1 action is based on membrane damage but not on the inhibition of bacterial translation. The structural analysis of prepronicomicins reveals that the BRICHOS domain does not exclusively participate in biosynthesis of β-hairpin polychaeta AMPs, but could also be a part of precursor of α-helical AMPs, namely nicomicins. Polyphemusin III is β-hairpin AMP that caused fast permeabilization of the cytoplasmic membrane of human leukemia cells HL-60. Flow cytometry experiments for annexin V-FITC / propidium iodide double staining revealed that the caspase inhibitor, Z-VAD-FMK, did not abrogate disruption of the plasma membrane by polyphemusin III. Our data suggest that polyphemusin III disrupts the plasma membrane integrity and induces cell death that is apparently not related to apoptosis. In comparison to known polyphemusins and tachyplesins, polyphemusin III demonstrates a similar or lower antibacterial effect, but significantly higher cytotoxicity against human cancer and transformed cells in vitro.

The first recombinant viper three finger toxins - antagonists of the nicotinic acetylcholine receptors of muscle and neuronal types

In collaboration with Laboratory of ligand-receptor interactions,  Laboratory of Molecular Diagnostics,  Laboratory of molecular toxinology

One of the main components of the venom of the snakes from the Elapidae family are three finger toxins, which possess various types of biological activity, including inhibition of synaptic transmission by blocking the nicotinic acetylcholine receptors. So far, three finger toxins have not been found in the venom of the Viperidae snakes, although the mRNA encoding these toxins has been found in the venom glands of snakes from this family. Genes encoding two three-finger toxins TFT-AF and TFT-VN, nucleotide sequences of which were earlier determined by cloning cDNA from venom glands of vipers Azemiops feae and Vipera nikolskii, respectively, were expressed for the first time in E. coli cells. The biological activity of these toxins was studied by electrophysiological techniques, calcium imaging, and radioligand analysis. We have shown for the first time that viper three-finger toxins are antagonists of nicotinic acetylcholine receptors both of neuronal and muscle type.

Dimerization of β-hairpin antimicrobial peptides plays a key role in the cytotoxicity but not in the antibacterial activity

In this investigation we performed the structure-activity relationship study of arenicin-1 to design less cytotoxic analogs of the peptide. More than 50 modified arenicin-1 analogs were obtained using methods of directed mutagenesis and heterologous expression in bacteria. The comparative evaluation of the impact of different biophysical parameters such as hydrophobicity, net charge, and β-hairpin length was carried out. Taken together, the obtained data suggest that dimerization rather than high hydrophobicity is the key reason of the cytotoxicity of natural β-hairpin antimicrobial peptide. The pronounced bactericidal activity against pathogens including extensively drug resistant ones as well as a wider therapeutic window makes arenicin analogs promising broad-spectrum antibacterial agents.

The mechanism of lipid binding and transfer by plant lipid-transfer proteins

In collaboration with Laboratory of biomolecular NMR-spectroscopy,  Laboratory of structural biology of ion channels

The lentil lipid transfer protein, designated as Lc-LTP2, was isolated from the Lens culinaris seeds. The protein belongs to LTP1 subfamily and consists of 93 amino acid residues. Its spatial structure contains four α-helices (H1-H4) and a long C-terminal tail. Here, we report the ligand-binding properties of Lc-LTP2. The fluorescent TNS binding assay revealed that the Lc-LTP2 affinity for saturated and unsaturated fatty acids was enhanced with a decrease in acyl chain length. Measurements of boundary potential in planar lipid bilayers and calcein dye-leakage in vesicular systems revealed preferential interaction of Lc-LTP2 with the negatively charged membranes. Lc-LTP2 more efficiently transferred anionic dimyristoyl-phosphatidylglycerol (DMPG) than zwitterionic dimyristoyl-phosphatidylcholine (DMPC). NMR experiments confirmed the higher affinity of Lc-LTP2 for anionic lipids and the ones with smaller volumes of hydrophobic chains. The acyl chains of the bound lyso-palmitoyl-phosphatidylglycerol (LPPG), DMPG, or dihexanoyl-phosphatidylcholine molecules occupied the internal hydrophobic cavity, while their head groups protruded into aqueous environment between H1 and H3 helices. The spatial structure and backbone dynamics of the Lc-LTP2/LPPG complex were determined. The internal cavity was expanded from ~600 to ~1000 А3 upon the protein ligation. Another entrance into the internal cavity, restricted by the H2-H3 interhelical loop and C-terminal tail, appeared to be responsible for the Lc-LTP2 attachment to the membrane or micelle surface and probably played an important role in the lipid uptake determining the ligand specificity. Our results confirmed previous assumption regarding the membrane-mediated antimicrobial action of Lc-LTP2 and afforded molecular insight into its biological role in the plant.