Кашеверов Игорь Евгеньевич

Доктор химических наук


Руководитель подразделения (Лаборатория лиганд-рецепторных взаимодействий)

Тел.: +7 (495) 995-55-57

Эл. почта: iekash@mx.ibch.ru

Избранные публикации

  1. Yu J, Zhu X, Zhang L, Kudryavtsev D, Kasheverov I, Lei Y, Zhangsun D, Tsetlin V, Luo S (2018). Species specificity of rat and human α7 nicotinic acetylcholine receptors towards different classes of peptide and protein antagonists. Neuropharmacology 139 (0), 226–237
  2. Kudryavtsev DS, Spirova EN, Shelukhina IV, Son LV, Makarova YV, Utkina NK, Kasheverov IE, Tsetlin VI (2018). Makaluvamine G from the Marine Sponge Zyzzia fuliginosa Inhibits Muscle nAChR by Binding at the Orthosteric and Allosteric Sites. Mar Drugs 16 (4),
  3. Durek T, Shelukhina IV, Tae HS, Thongyoo P, Spirova EN, Kudryavtsev DS, Kasheverov IE, Faure G, Corringer PJ, Craik DJ, Adams DJ, Tsetlin VI (2017). Interaction of Synthetic Human SLURP-1 with the Nicotinic Acetylcholine Receptors. Sci Rep 7 (1), 16606
  4. Vulfius CA, Kasheverov IE, Kryukova EV, Spirova EN, Shelukhina IV, Starkov VG, Andreeva TV, Faure G, Zouridakis M, Tsetlin VI, Utkin YN (2017). Pancreatic and snake venom presynaptically active phospholipases A2inhibit nicotinic acetylcholine receptors. PLoS One 12 (10), e0186206
  5. Kasheverov IE, Chugunov AO, Kudryavtsev DS, Ivanov IA, Zhmak MN, Shelukhina IV, Spirova EN, Tabakmakher VM, Zelepuga EA, Efremov RG, Tsetlin VI (2016). High-Affinity α-Conotoxin PnIA Analogs Designed on the Basis of the Protein Surface Topography Method. Sci Rep 6 (0), 36848
  6. Lyukmanova EN, Shulepko MA, Shenkarev ZO, Kasheverov IE, Chugunov AO, Kulbatskii DS, Myshkin MY, Utkin YN, Efremov RG, Tsetlin VI, Arseniev AS, Kirpichnikov MP, Dolgikh DA (2016). Central loop of non-conventional toxin WTX from Naja kaouthia is important for interaction with nicotinic acetylcholine receptors. Toxicon 119 (0), 274–279
  7. Stepanov A, Belyy A, Kasheverov I, Rybinets A, Dronina M, Dyachenko I, Murashev A, Knorre V, Sakharov D, Ponomarenko N, Tsetlin V, Tonevitsky A, Deyev S, Belogurov A, Gabibov A (2016). Development of a recombinant immunotoxin for the immunotherapy of autoreactive lymphocytes expressing MOG-specific BCRs. Biotechnol Lett 38 (7), 1173–1180
  8. Zakharova GV, Konstantinov RR, Odinokov AV, Chibisov AK, Alfimov MV, Kasheverov IE, Utkin YN, Zhmak MN, Tsetlin VI (2016). Effect of a peptide modeling the nicotinic receptor binding site on the spectral and luminescent properties of dye complexes with cucurbit[8]uril. HIGH ENERG CHEM+ 50 (2), 121–126
  9. Lin B, Xu M, Zhu X, Wu Y, Liu X, Zhangsun D, Hu Y, Xiang SH, Kasheverov IE, Tsetlin VI, Wang X, Luo S (2016). From crystal structure of α-conotoxin GIC in complex with Ac-AChBP to molecular determinants of its high selectivity for α3β2 nAChR. Sci Rep 6 (0), 22349
  10. Lyukmanova EN, Shulepko MA, Kudryavtsev D, Bychkov ML, Kulbatskii DS, Kasheverov IE, Astapova MV, Feofanov AV, Thomsen MS, Mikkelsen JD, Shenkarev ZO, Tsetlin VI, Dolgikh DA, Kirpichnikov MP (2016). Human secreted Ly-6/uPAR related protein-1 (SLURP-1) is a selective allosteric antagonist of α7 nicotinic acetylcholine receptor. PLoS One 11 (2), e0149733
  11. Dyshlovoy SA, Otte K, Alsdorf WH, Hauschild J, Lange T, Venz S, Bauer CK, Bähring R, Amann K, Mandanchi R, Schumacher U, Schröder-Schwarz J, Makarieva TN, Guzii AG, Tabakmakher KM, Fedorov SN, Shubina LK, Kasheverov IE, Ehmke H, Steuber T, Stonik VA, Bokemeyer C, Honecker F, von Amsberg G (2016). Marine compound rhizochalinin shows high in vitro and in vivo efficacy in castration resistant prostate cancer. Oncotarget 7 (43), 69703–69717
  12. Kudryavtsev DS, Shelukhina IV, Son LV, Ojomoko LO, Kryukova EV, Lyukmanova EN, Zhmak MN, Dolgikh DA, Ivanov IA, Kasheverov IE, Starkov VG, Ramerstorfer J, Sieghart W, Tsetlin VI, Utkin YN (2015). Neurotoxins from snake venoms and α-Conotoxin ImI inhibit functionally active Ionotropic γ-aminobutyric acid (GABA) receptors. J Biol Chem 290 (37), 22747–22758
  13. Kasheverov IE, Shelukhina IV, Kudryavtsev DS, Makarieva TN, Spirova EN, Guzii AG, Stonik VA, Tsetlin VI (2015). 6-Bromohypaphorine from marine nudibranch mollusk Hermissenda crassicornis is an agonist of human α7 nicotinic acetylcholine receptor. Mar Drugs 13 (3), 1255–1266
  14. Vulfius CA, Kasheverov IE, Starkov VG, Osipov AV, Andreeva TV, Filkin SY, Gorbacheva EV, Astashev ME, Tsetlin VI, Utkin YN (2014). Inhibition of Nicotinic Acetylcholine Receptors, a Novel Facet in the Pleiotropic Activities of Snake Venom Phospholipases A2. PLoS One 9 (12), e115428
  15. Kudryavtsev D, Makarieva T, Utkina N, Santalova E, Kryukova E, Methfessel C, Tsetlin V, Stonik V, Kasheverov I (2014). Marine natural products acting on the acetylcholine-binding protein and nicotinic receptors: From computer modeling to binding studies and electrophysiology. Mar Drugs 12 (4), 1859–1875
  16. Lyukmanova EN, Shulepko MA, Buldakova SL, Kasheverov IE, Shenkarev ZO, Reshetnikov RV, Filkin SY, Kudryavtsev DS, Ojomoko LO, Kryukova EV, Dolgikh DA, Kirpichnikov MP, Bregestovski PD, Tsetlin VI (2013). Water-soluble LYNX1 residues important for interaction with muscle-type and/or neuronal nicotinic receptors. J Biol Chem 288 (22), 15888–15899
  17. Shulepko MA, Lyukmanova EN, Paramonov AS, Lobas AA, Shenkarev ZO, Kasheverov IE, Dolgikh DA, Tsetlin VI, Arseniev AS, Kirpichnikov MP (2013). Human neuromodulator SLURP-1: Bacterial expression, binding to muscle-type nicotinic acetylcholine receptor, secondary structure, and conformational heterogeneity in solution. Biochemistry (Mosc) 78 (2), 204–211
  18. Utkin YN, Weise C, Kasheverov IE, Andreeva TV, Kryukova EV, Zhmak MN, Starkov VG, Hoang NA, Bertrand D, Ramerstorfer J, Sieghart W, Thompson AJ, Lummis SCR, Tsetlin VI (2012). Azemiopsin from Azemiops feae viper venom, a novel polypeptide ligand of nicotinic acetylcholine receptor. J Biol Chem 287 (32), 27079–27086
  19. Surin AM, Kryukova EV, Strukov AS, Zhmak MN, Talka R, Tuominen R, Salminen O, Khiroug LS, Kasheverov IE, Tsetlin VI (2012). The effect of MII α-conotoxin and its N-terminal derivatives on Ca2+- and Na+-signals induced by nicotine in SH-SY5Y neuroblastoma cells. Russ. J. Bioorganic Chem. 38 (2), 184–191
  20. Osipov AV, Rucktooa P, Kasheverov IE, Filkin SY, Starkov VG, Andreeva TV, Sixma TK, Bertrand D, Utkin YN, Tsetlin VI (2012). Dimeric α-Cobratoxin X-ray structure: Localization of intermolecular disulfides and possible mode of binding to nicotinic acetylcholine receptors. J Biol Chem 287 (9), 6725–6734
  21. Kasheverov IE, Zhmak MN, Khruschov AY, Tsetlin VI (2011). Design of new α-conotoxins: From computer modeling to synthesis of potent cholinergic compounds. Mar Drugs 9 (10), 1698–1714
  22. Shulepko MA, Lyukmanova EN, Kasheverov IE, Dolgikh DA, Tsetlin VI, Kirpichnikov MP (2011). Bacterial expression of the water-soluble domain of lynx1, an endogenous neuromodulator of human nicotinic receptors. Russ. J. Bioorganic Chem. 37 (5), 543–549
  23. Vulfius CA, Gorbacheva EV, Starkov VG, Osipov AV, Kasheverov IE, Andreeva TV, Astashev ME, Tsetlin VI, Utkin YN (2011). An unusual phospholipase A2from puff adder Bitis arietans venom - a novel blocker of nicotinic acetylcholine receptors. Toxicon 57 (5), 787–793
  24. Lyukmanova EN, Shenkarev ZO, Shulepko MA, Mineev KS, DHoedt D, Kasheverov IE, Filkin SY, Krivolapova AP, Janickova H, Dolezal V, Dolgikh DA, Arseniev AS, Bertrand D, Tsetlin VI, Kirpichnikov MP (2011). NMR structure and action on nicotinic acetylcholine receptors of water-soluble domain of human LYNX1. J Biol Chem 286 (12), 10618–10627
  25. Tsetlin V, Kuzmin D, Kasheverov I (2011). Assembly of nicotinic and other Cys-loop receptors. J Neurochem 116 (5), 734–741
  26. Шулепко МА, Люкманова ЕН, Кашеверов ИЕ, Долгих ДА, Цетлин ВИ, Кирпичников МП (2011). Бактериальная продукция водорастворимого домена lynx1, - эндогенного нейромодулятора никотиновых рецепторов человека. 37 (5),
  27. Kasheverov IE, Zhmak MN, Fish A, Rucktooa P, Khruschov AY, Osipov AV, Ziganshin RH, DHoedt D, Bertrand D, Sixma TK, Smit AB, Tsetlin VI (2009). Interaction of α-conotoxin ImII and its analogs with nicotinic receptors and acetylcholine-binding proteins: Additional binding sites on Torpedo receptor. J Neurochem 111 (4), 934–944
  28. Tsetlin V, Utkin Y, Kasheverov I (2009). Polypeptide and peptide toxins, magnifying lenses for binding sites in nicotinic acetylcholine receptors. Biochem Pharmacol 78 (7), 720–731
  29. Lyukmanova EN, Shulepko MA, Tikhonov RV, Shenkarev ZO, Paramonov AS, Wulfson AN, Kasheverov IE, Ustich TL, Utkin YN, Arseniev AS, Tsetlin VI, Dolgikh DA, Kirpichnikov MP (2009). Bacterial production and refolding from inclusion bodies of a "weak" toxin, a disulfide rich protein. Biochemistry (Mosc) 74 (10), 1142–1149
  30. Kasheverov IE, Utkin YN, Tsetlin VI (2009). Naturally occurring and synthetic peptides acting on nicotinic acetylcholine receptors. Curr Pharm Des 15 (21), 2430–2452
  31. Osipov AV, Kasheverov IE, Makarova YV, Starkov VG, Vorontsova OV, Ziganshin RK, Andreeva TV, Serebryakova MV, Benoit A, Hogg RC, Bertrand D, Tsetlin VI, Utkin YN (2008). Naturally occurring disulfide-bound dimers of three-fingered toxins: A paradigm for biological activity diversification. J Biol Chem 283 (21), 14571–14580
  32. Kasheverov IE, Zhmak MN, Vulfius CA, Gorbacheva EV, Mordvintsev DY, Utkin YN, Van Elk R, Smit AB, Tsetlin VI (2006). α-Conotoxin analogs with additional positive charge show increased selectivity towards Torpedo californica and some neuronal subtypes of nicotinic acetylcholine receptors. FEBS J 273 (19), 4470–4481
  33. Mordvintsev DY, Polyak YL, Kuzmine DA, Levtsova OV, Tourleigh YV, Kasheverov IE (2006). A model for short α-neurotoxin bound to nicotinic acetylcholine receptor from Torpedo californica. J Mol Neurosci 30 (12), 71–72
  34. Tsetlin VI, Kasheverov IE, Zhmak MN, Utkin YN, Vulfius CA, Smit AB, Bertrand D (2006). α-conotoxin analogs with enhanced affinity for nicotinic receptors and acetylcholine-binding proteins. J Mol Neurosci 30 (12), 77–78
  35. Smit AB, Celie PHN, Kasheverov IE, Mordvintsev DY, Van Nierop P, Bertrand D, Tsetlin V, Sixma TK (2006). Acetylcholine-binding proteins: Functional and structural homologs of nicotinic acetylcholine receptors. J Mol Neurosci 30 (12), 9–10
  36. Shelukhina IV, Kryukova EV, Skok MV, Lykhmus EY, Zhmak MN, Mordvintsev DY, Kasheverov IE, Tsetlin VI (2006). Analysis of specificity of antibodies against synthetic fragments of different neuronal nicotinic acetylcholine receptor subunits. Biochemistry (Mosc) 71 (7), 749–758
  37. Kasheverov IE, Chiara DC, Zhmak MN, Maslennikov IV, Pashkov VS, Arseniev AS, Utkin YN, Cohen JB, Tsetlin VI (2006). α-conotoxin GI benzoylphenylalanine derivatives 1H-NMR structures and photoaffinity labeling of the Torpedo californica nicotinic acetylcholine receptor. FEBS J 273 (7), 1373–1388
  38. Kasheverov IE, Utkin YN, Tsetlin VI (2006). Natural α-conotoxins and their synthetic analogues in study of nicotinic acetylcholine receptors. Russ. J. Bioorganic Chem. 32 (2), 103–115
  39. Mordvintsev DY, Polyak YL, Levtsova OV, Tourleigh YV, Kasheverov IE, Shaitan KV, Utkin YN, Tsetlin VI (2005). A model for short α-neurotoxin bound to nicotinic acetylcholine receptor from Torpedo californica: Comparison with long-chain α-neurotoxins and α-conotoxins. Comput Biol Chem 29 (6), 398–411
  40. Celie PHN, Kasheverov IE, Mordvintsev DY, Hogg RC, Van Nierop P, Van Elk R, Van Rossum-Fikkert SE, Zhmak MN, Bertrand D, Tsetlin V, Sixma TK, Smit AB (2005). Crystal structure of nicotinic acetylcholine receptor homolog AChBP in complex with an α-conotoxin PnIA variant. Nat Struct Mol Biol 12 (7), 582–588
  41. Vulfius CA, Tumina OB, Kasheverov IE, Utkin YN, Tsetlin VI (2005). Diversity of nicotinic receptors mediating Cl-current in Lymnaea neurons distinguished with specific agonists and antagonist. Neurosci Lett 373 (3), 232–236
  42. Kasheverov IE, Zhmak MN, Maslennikov IV, Utkin YN, Tsetlin VI (2003). A comparative study on selectivity of α-conotoxins GI and ImI using their synthetic analogues and derivatives. Neurochem Res 28 (34), 599–606
  43. Kasheverov IE, Rozhkova AM, Zhmak MN, Utkin YN, Tsetlin VI (2002). Photoactivatable analogues of α-conotoxins GI and MI and their interaction with nicotinic acetylcholine receptor. Russ. J. Bioorganic Chem. 28 (2), 86–92
  44. Kasheverov IE, Rozhkova AM, Zhmak MN, Utkin YN, Tsetlin VI (2002). Photoactivatable analogues of α-conotoxins GI and MI and their interaction with nicotinic acetylcholine receptor. Bioorg Khim 28 (2), 107–108
  45. Tsetlin VI, Dergousova NI, Azeeva EA, Kryukova EV, Kudelina IA, Shibanova ED, Kasheverov IE, Methfessel C (2002). Refolding of the Escherichia coli expressed extracellular domain of α7 nicotinic acetylcholine receptor. Cys116 mutation diminishes aggregation and stabilizes the β structure. 269 (11), 2801–2809
  46. Zhmak MN, Kasheverov IE, Utkin YN, Tsetlin VI, Volpina OM, Ivanov VT (2001). An Efficient Synthetic Scheme for Natural α-Conotoxins and Their Analogues. Russ. J. Bioorganic Chem. 27 (2), 67–71
  47. Utkin YN, Kasheverov IE, Tsetlin VI (2001). α-Neurotoxins and α-conotoxins, blockers of nicotinic acetylcholine receptors. Bioorg Khim 25 (11), 805–810
  48. Zhmak MN, Kasheverov IE, Utkin YN, Tsetlin VI, Volpina OM, Ivanov VT (2001). An efficient synthetic scheme for natural α-conotoxins and their analogues. Bioorg Khim 27 (2), 87–88
  49. Zolotarev YA, Bocharov EV, Dadayan AK, Kasheverov IE, Zhmak MN, Maslennikov IV, Borisov YA, Arseniev AS, Myasoedov NF, Tsetlin VI (2001). The solid-state catalytic isotope exchange of hydrogen in α-conotoxin G1 by the tritium spillover. Bioorg Khim 26 (8), 591–592
  50. Kasheverov I, Rozhkova A, Zhmak M, Utkin Y, Ivanov V, Tsetlin VI (2001). Photoactivatable α-conotoxins reveal contacts with all subunits as well as antagonist-induced rearrangements in the Torpedo californica acetylcholine receptor. 268 (13), 3664–3673
  51. Zolotarev YA, Bocharov EV, Dadayan AK, Kasheverov IE, Zhmak MN, Maslennikov IV, Borisov YA, Arseniev AS, Myasoedov NF, Tsetlin VI (2000). The solid-state catalytic isotope exchange of hydrogen in α-conotoxin G1 by the tritium spillover. Bioorg Khim 26 (8), 587–592
  52. Zolotarev YA, Bocharov EV, Dadayan AK, Kasheverov IE, Zhmak MN, Maslennikov IV, Borisov YA, Arseniev AS, Myasoedov NF, Tsetlin VI (2000). The solid-state catalytic isotope exchange of hydrogen in α-conotoxin G1 by the tritium spillover. Russ. J. Bioorganic Chem. 26 (8), 527–531
  53. Utkin YN, Kasheverov IE, Tsetlin VI (1999). α-neurotoxins and α-conotoxins, blockers of nicotinic acetylcholine receptors. Russ. J. Bioorganic Chem. 25 (11), 711–716
  54. Kasheverov I, Zhmak M, Chivilyov E, Saez-Brionez P, Utkin Y, Hucho F, Tsetlin V (1999). Benzophenone-type photoactivatable derivatives of α-neurotoxins and α- conotoxins in studies on Torpedo nicotinic acetylcholine receptor. J Recept Signal Transduct Res 19 (14), 559–571
  55. Utkin YN, Krivoshein AV, Davydov VL, Kasheverov IE, Franke P, Maslennikov IV, Arseniev AS, Hucho F, Tsetlin VI (1998). Labeling of Torpedo californica nicotinic acetylcholine receptor subunits by cobratoxin derivatives with photoactivatable groups of different chemical nature at Lys23. 253 (1), 229–235
  56. Kasheverov I, Utkin Y, Weise C, Franke P, Hucho F, Tsetlin V (1998). Reverse-phase chromatography isolation and MALDI mass spectrometry of the acetylcholine receptor subunits. Protein Expr Purif 12 (2), 226–232
  57. Kasheverov IE, Utkin YN, Franke P, Tsetlin VI (1997). Substance P derivatives with photoactivatable labels in the N-terminal part of the molecule. Chem Biol Drug Des 50 (6), 408–414
  58. Kasheverov IE, Utkin YN, Tsetlin VI (1997). Antagonists of the Tachykinin Receptors. Bioorg Khim 23 (6), 464–468
  59. Kasheverov IE, Utkin YN, Tsetlin VI (1997). Antagonists of the tachykinin receptors. Russ. J. Bioorganic Chem. 23 (6), 419–435
  60. Utkin YN, Lazakovich EM, Kasheverov IE, Tsetlin VI (1989). α-Bungarotoxin interacts with the rat brain tachykinin receptors. FEBS Lett 255 (1), 111–115