Laboratory of optical microscopy and spectroscopy of biomolecules
The Laboratory studies biologically active molecules, membranous and membrane-active proteins and develops new methods of optical microscopy and spectroscopy to support these studies. Developed methods are used to study the action mechanisms and structural-functional interrelationships of investigated molecules at different levels of structural organization: molecular, cellular and tissue.
The methods of microscopy of single-molecules and their complexes are developed in the Laboratory using the unique scientific equipment. It includes an installation for one-photon and multiphoton 4Pi superresolution microscopy and an installation for fluorescence microscopy based on the effect of total internal reflection. Both installations have got a high sensitivity, allowing registering fluorescent signals even from single molecules.
Experimental installation for confocal laser microspectroscopy was created in the Laboratory. Also, the method of confocal microspectroscopy and reconstruction of spectral images (COMIRSI) was developed that provided possibility to identify and study molecular interactions of biologically active compounds in living cells with a three-dimensional submicron spatial resolution. The experimental installation and the COMIRSI method are widely used in the development, scientific and clinical studies of new Russian photosensitizers for photodynamic cancer therapy, as well as in the studies of membrane and membrane-active proteins.
The Laboratory is open for cooperation with the departments of the Institute and other scientific organizations.
The development of analytical systems based on hybrid ion channels for the search and study of potassium channel blockers is conducted jointly with the Group of nanobioengineering. A search and study of potassium channel blockers in scorpion venoms are carried out in collaboration with the Molecular Instruments for Neurobiology Group. The functional properties of toxins from snake venom are studied together with the Laboratory of Molecular Toxinology.
Also, the Laboratory conducts joint studies with a number of other scientific organizations. Study of photosensitizers for photodynamic anti-cancer and antimicrobial therapy and optimization of their properties is carried out in cooperation with the Moscow Technological University, the P.A. Herzen Moscow Oncology Research Institute and the State Scientific Center "NIOPIK". Studies of structural rearrangements in nucleosomes under the influence of various nuclear proteins using the methods of fluorescence microscopy of single molecules and their complexes are carried out together with the Bioengineering Department, the Laboratory for the Regulation of Transcription and Replication of the Biological Faculty of Moscow State University and colleagues from Fox Chase Cancer Center (USA). The structure optimization of conjugates of boron-containing nanoparticles and natural porphyrins is conducted jointly with the Moscow Technological University and INEOS RAS.
The laboratory collaborates with foreign scientific organizations. Biomedical nanosensors for the diagnosis and treatment of breast cancer are being developed together with the University of Tours (France) and the Rhine-Waal University of Applied Sciences (Germany).
The Laboratory employees teach the basics of optical microscopy to students of the ESC of the IBCh RAS and the Department of Bioengineering of the Biological Faculty of the Moscow State University. Each year, several bachelor's and master's works as well as scientific research of graduate students and young scientists are conducted. under the supervision of the Laboratory employees
The Laboratory of optical microscopy and spectroscopy of biomolecules was established in 2005. It is a part of the Department of Structural Biology, which arose as a result of reorganization of the Laboratory of Instrumental Methods of Analysis. This reorganization was conducted due to the initiative of professor A.S. Arsenyev, who is the current head of the Department.
A three-dimensional confocal image of HEK293 cells in which a stable expression of the EphA2 receptor of EphA2 fused to a cyan fluorescent protein (shown in green) is achieved, together with EphA2 fused to a yellow fluorescent protein (shown in red). The coincidence of the two structures localization is shown in yellow. The cell model is used to study the mechanisms of activation and dimerization of Efrin receptors using the method of resonant fluorescence energy transfer (FRET).
- Cellular bioengineering systems and methods of their applications using fluorescence microscopy have been developed. They are used to study pore blockers of voltage-gated potassium channels and to search for peptide blockers in natural venoms.
- Using fluorescent proteins and scorpion toxins, the high-affinity conjugates were designed that interact selectively with Kv1 channels and can be used for their visualization.
- New high-affinity channel blockers of Kv1.x (x = 1-3.6) were discovered in scorpion venoms and characterized by activity.
- Recombinant peptide-blockers of Kv-channels having improved selectivity were created. Molecular models of peptide-blockers complexes with Kv-channels were constructed.; Interaction interfaces and amino acid residues affecting the force and selectivity of interactions were described.
- Methods for studying single molecules and their complexes in the free diffusion regime and in the immobilized state have been developed based on Forster resonance energy transfer microscopy. Applicability of these methods for investigation of structural changes of nucleosomal DNA in complexes with different protein factors was demonstrated.
- Structures of new conjugates of boron-containing nanoparticles and natural porphyrins have been optimized that provided delivery of more than 1 billion boron atoms per cancer cell. In this way, applicability of new nanoconjugates for boron-neutron capture therapy (BNCT) was provided. Nanoconjugates are active photosensitizers and cause photoinduced death of tumor cells at nanomolar concentrations. New conjugates are perspective multifunctional agents for photodynamic therapy, BNСT and fluorescent cancer diagnostics.
- It has been established that the key structural element determining high accumulation of chlorin e6 conjugates with cobalt bis(dicarbolide) nanoparticle in cancer cells is the amino-polyalkyl-amine linker that connects a porphyrin chromophore to nanoparticle. Photocytotoxicity of conjugates is based on photoinduced lipid peroxidation, lysosomes permeabilization, and proteases activation in cytoplasm of cells.
|Alexey Feofanov, D.Sc, assistant firstname.lastname@example.org, |
|George Sharonov, Ph.D.||s. r. email@example.com, |
|Anastasia Efremenko||r. firstname.lastname@example.org|
|Anastasia Ignatova||r. email@example.com, |
|Kseniya Markvicheva, Ph.D.||j. r. firstname.lastname@example.org, |
|Anna Feofanova||t. q. - lab. as.|
|Marija Astapova, Ph.D.||s. r. email@example.com|
Mechanisms of anticancer action of curaxins (2019-12-09)
Data of spFRET analysis support the hypothesis that anticancer drug curaxin, namely, its CBL0137 derivative, can affect long-distance enhancer-promoter communication (EPC) in chromatin by disrupting nucleosome structure or affecting the structure and dynamics of the linker DNA supporting efficient EPC (Kantidze et al., Nat Commun., 2019,10(1):1441). The data indicate also that CBL0137 attracts human FACT (protein factor that FAcilitates Chromatin Transcription) to nucleosomes, mediates hFACT-induced scaled, partially reversible nucleosome unfolding (or uncoiling of the nucleosomal DNA) and traps hFACT on nucleosomes. This curaxin-dependent FACT trapping can be a reason of hFACT redistribution from the transcribed chromatin regions to other genomic loci and contribute to the anticancer action of curaxins (Chang et al. Science Advances, 2018, 4 (11), eaav2131).
The studies were performed jointly with the specialists from the Institute of Gene Biology RAS (Kantidze O.L., Luzhin A.V., Golov A.K., Velichko A.K.), Biology Faculty of Lomonosov Moscow State University (Valieva M.E., Lyubitelev A.V., Razin S.V.), Fox Chase Cancer Center, USA (Nizovtseva E.V., Studitsky V.M., Kulaeva O.I., Chang H.-W.), Roswell Park Comprehensive Cancer Center, USA (Gurova K.V., Safina A., Wang J.,), Eunice Kennedy Shriver National Institute for Child Health and Human Development, USA (Chereji R.V.), Rutgers University, USA (Morozov A.V.).
- (2019). The anti-cancer drugs curaxins target spatial genome organization. Nat Commun 10 (1), 1441
- (2018). Mechanism of FACT removal from transcribed genes by anticancer drugs curaxins. Sci Adv 4 (11), eaav2131
MeKTx11-1, Kv1.2 channel –specific peptide blocker from the M.eupeus scorpion venom: structural basis of selectivity (2018-11-30)
A.V. Feofanov (Laboratory of optical microscopy and spectroscopy of biomolecules), О.V. Nekrasova, K.S.Kudryashova (Group of nanobioengineering, Bioengineering department), A.A. Vassilevski, A.I. Kuzmenkov, A.M. Gigolaev (Laboratory of molecular instruments for neurobiology), A.O. Chugunov, V.M. Tabakmakher, R.G. Efremov (Group of in silico analysis of membrane proteins structure, Laboratory of biomolecular modeling).
A unique high-affinity and highly selective peptide blocker of Kv1.2 channel, MeKTx11-1, from the scorpion venom Mesobuthus eupeus was studied. Peptide MeKTx11-1 and its mutant forms were produced in a recombinant form, and their receptor-binding activity was studied against a panel of Kv1-channels. Molecular modeling of interaction of these peptides with Kv1.2 channel was carried out, and key structural elements of the interactions were determined. Peptide MeKTx11-1 may be used as a novel efficient molecular tool in neurobiology to identify and study the activity of Kv1.2 channel in the presence of different isoforms of Kv1-channels.
In collaboration with S.Peigneur and J.Tytgat fromUniversity of Leuven, Belgium and A.F. Fradkov from Evrogen JSC.
- (2018). K1.2 channel-specific blocker from Mesobuthus eupeus scorpion venom: Structural basis of selectivity. Neuropharmacology 143, 228–238
Mammalian three-finger proteins protect against cancer (2018-11-30)
Efremenko A.V., Sharonov G.V., Feofanov A.V. (Laboratory of optical microscopy and spectroscopy of biomolecules), Lyukmanova E.N., Bychkov M.L., Shulepko M.A., Kulbatskii D.S., Dolgikh D.A., Kirpichnikov M.P. (Group of bioengineering of neuromodulators and neuroreceptors), Shenkarev Z.O. (Group of structural biology of ion channels).
The human secreted protein SLURP-1, which is expressed in epithelial cells and controls their proliferation and migration, has been found to inhibit the growth of epithelial cancer cells. The effect of SLURP-1 on cancer cells is characterized by a positive feedback: exogenous (recombinant) SLURP-1 binds to α7 nicotinic acetylcholine receptors on the cell membrane and triggers a cascade of signals that activates secretion of endogenous SLURP-1 from intracellular depot, quickly increasing its concentration in the intercellular space and enhancing antiproliferative action. Concentrations of SLURP-1, which suppress the growth of cancer cells, do not affect the growth of normal cells.
- (2018). Human secreted proteins SLURP-1 and SLURP-2 control the growth of epithelial cancer cells via interactions with nicotinic acetylcholine receptors. Br J Pharmacol 175 (11), 1973–1986
Internalization mechanisms and intracellular distribution features of magnetic nanoparticles functionalized with folic acid (2017-11-27)
Ignatova A.A., Feofanov A.V.
Theranostics-oriented fluorescently-labeled iron oxide nanoparticles coated with polyethylene glycol and functionalized with folic acid effectively accumulate in HeLa cervix carcinoma cells having a high level of membrane folate receptors.
Penetration of nanoparticles into HeLa cells occurs primarily by clathrin-dependent endocytosis with a weak participation of caveolin-mediated endocytosis and ends with their accumulation in lysosomes.
This work was performed in collaboration with Shebanova A. (Biological Faculty, Lomonosov Moscow State University), Allard-Vannier E., Hervé-Aubert K., Kaaki K., Blondy T., Saboungi M.L., Chourpa I.(EA 6295 Nanomédicaments et Nanosondes, Université F. Rabelais de Tours, Tours, France).
- (2017). Folic acid-capped PEGylated magnetic nanoparticles enter cancer cells mostly via clathrin-dependent endocytosis. BIOCHIM BIOPHYS ACTA 1861 (6), 1578–1586
AN EFFICIENT METHOD FOR PRODUCTION OF RECOMBINANT α-КТХ PEPTIDES – THE BLOCKERS OF POTASSIUM CHANNELS (2017-11-27)
O.V.Nekrasova, K.S.Kudryashova, S.A.Yakimov, M.P.Kirpichnikov
A bioengineering method for production of peptide blockers of potassium Kv1 channels has been developed that provides:
- high yield of the target peptides (12-22 mg/l culture);
- retaining the native amino acid sequence of α-КТх peptides;
- high yield of the renatured form of the peptides with correctly formed three and four disulfide bonds;
- simple and reliable procedure of peptide isolation and purification.
The recombinant peptides of the α-KTx family obtained by this method have the activity of the natural blockers. High affinity potassium channel blockers from scorpion venom are widely used to study the structure and function of the channels and have a promising medical significance.
- (2017). Recombinant scorpion toxins: Focus on four-disulfide peptide blockers of Kv1-channels. Bioengineered 9 (1), 25–29
- (2017). Straightforward approach to produce recombinant scorpion toxins—Pore blockers of potassium channels. J Biotechnol 241, 127–135
Development of integrated transcriptomic and proteomic approach to search for blockers of potassium channels in animal venoms (2016-03-26)
Kuzmenkov A.I. , Vassilevski A.A., Grishin Eu.V.
Department of molecular neurobiology
Kudryashova K.S., Nekrasova O.V., Kirpichnikov M.P.
Laboratory of optical microscopy and spectroscopy of biomolecules
An original approach was developed to search for new ligands of potassium channels. It combines the bioengineering cellular test system and transcriptomic and proteomic analysis of animal venoms. Using this approach eight high-affinity peptide blockers of voltage-gated potassium channel Kv1.1 (including five new peptides) were found in the venom of the scorpion Mesobuthus eupeus. The proposed approach is a versatile and effective tool for directed search for blockers of potassium channels in natural venoms.
- (2015). Variability of potassium channel blockers in Mesobuthus eupeus scorpion venom with focus on Kv1.1: An integrated transcriptomic and proteomic study. J Biol Chem 290 (19), 12195–12209